Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-23T06:23:30.862Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of local irradiation with 630 and 860 nm low-level lasers on tympanic membrane perforation repair in guinea pigs

Published online by Cambridge University Press:  21 February 2013

Sh Maleki ,
S K Kamrava ,
D Sharifi ,
M Jalessi ,
A Asghari ,
S Ghalehbaghi  and
P Yazdanifard
Sh Maleki
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
S K Kamrava*
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
D Sharifi
Affiliation:
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
M Jalessi
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
A Asghari
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
S Ghalehbaghi
Affiliation:
Family Planning and Population Control Department, Islamic Azad University, South Tehran Branch, Tehran, Iran
P Yazdanifard
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
*
Address for correspondence: Dr Seyed Kamran Kamrava, Associate Professor of ENT–Head and Neck Surgery, Otolaryngology-Head and Neck Surgery Research Center, Rasoul Akram Hospital, Tehran University of Medical Sciences, Niayesh St, Sattarkhan Av, Tehran, Iran Fax: +98 21 66552828 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

In this study, we evaluated the effect of low-level lasers on the healing of tympanic membrane perforation, one of the most common otological pathologies.

Methods and materials:

Twenty-four guinea pigs were randomly assigned to either the experimental or control group. One day after the induction of a 2 mm diameter, centred myringotomy in all animals, the tympanic membranes in the experimental group were irradiated with 630 and 860 nm lasers for 10 days. Two weeks later, histological changes in the membranes were evaluated.

Results:

Tympanic membrane thickening and inflammatory cell infiltration in the tympanic membranes and surrounding tissues were significantly less in the experimental group (p < 0.001). The distance from the external auditory canal wall to the malleus tip did not differ significantly between the two groups (p = 0.42).

Conclusion:

The results show that the combined application of 630 and 860 nm lasers had a significant effect on the healing of tympanic membrane perforation, and on the prevention of thick fibrotic or atelectatic neomembrane formation.

Keywords

EarMiddleOtitis MediaTraumaTympanic MembraneWound HealingLasersGuinea Pigs
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 3 , March 2013 , pp. 260 - 264
Copyright
Copyright © JLO (1984) Limited 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Santa Maria, PL, Atlas, MD, Ghassemifar, R.Chronic tympanic membrane perforation: a better animal model is needed. Wound Repair Regen 2007;15:450–8CrossRefGoogle ScholarPubMed
2Golz, A, Goldenberg, D, Netzer, A, Fradis, M, Westerman, ST, Westerman, LM et al. Paper patching for chronic tympanic membrane perforations. Otolaryngol Head Neck Surg 2003;128:565–70CrossRefGoogle ScholarPubMed
3Chen, XW, Yang, H, Gao, RZ, Yu, R, Gao, ZQ.Perichondrium/cartilage composite graft for repairing large tympanic membrane perforations and hearing improvement. Chin Med J (Engl) 2010;123:301–4Google ScholarPubMed
4Wang, WQ, Wang, ZM, Chi, FL.Spontaneous healing of various tympanic membrane perforations in the rat. Acta Otolaryngol 2004;124:1141–4CrossRefGoogle ScholarPubMed
5Kim, JH, Bae, JH, Lim, KT, Choung, PH, Park, JS, Choi, SJ et al. Development of water-insoluble chitosan patch scaffold to repair traumatic tympanic membrane perforations. J Biomed Mater Res A 2009;90:446–55CrossRefGoogle ScholarPubMed
6Mudry, A.History of myringoplasty and tympanoplasty type I. Otolaryngol Head Neck Surg 2008;139:613–14CrossRefGoogle ScholarPubMed
7Levin, B, Redmond, SL, Rajkhowa, R, Eikelboom, RH, Marano, RJ, Atlas, MD.Preliminary results of the application of a silk fibroin scaffold to otology. Otolaryngol Head Neck Surg 2010;142(suppl 1):S33–5CrossRefGoogle ScholarPubMed
8Sheehy, JL, Anderson, RG.Myringoplasty: a review of 472 cases. Ann Otol 1980;89:331–4Google ScholarPubMed
9Wang, Y, Kim, HJ, Vunjak-Novakovic, G, Kaplan, DL.Stem cell-based tissue engineering with silk biomaterials. Biomaterials 2006;27:6064–82CrossRefGoogle ScholarPubMed
10Kim, J, Kim, CH, Park, CH, Seo, JN, Kweon, H, Kang, SW et al. Comparison of methods for the repair of acute tympanic membrane perforations: silk patch vs. paper patch. Wound Repair Regen 2010;18:132–8CrossRefGoogle ScholarPubMed
11Ribari, O, Jori, J, Kiss, JG, Vona, I, Tomity, I.Closure of tympanic perforations with low-energy He-Ne-laser irradiation [in German]. Acta Chir Acad Sci Hung 1980;21:229–38Google ScholarPubMed
12Chen, AC, Arany, PR, Huang, YY, Tomkinson, EM, Sharma, SK, Kharkwal, GB et al. Low-level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts. PLoS One 2011;6:e22453Google ScholarPubMed
13Tuner, J, Hode, L.The Laser Therapy Handbook. Grangesberg, Sweden: Prima Books, 2004Google Scholar
14Pfalz, R.Suitability of various lasers for interventions from the tympanic membrane to the foot plate (Er:YAG, argon, CO2 s.p.–, Ho:YAG laser) [in German]. Laryngorhinootologie 1995;74:21–5CrossRefGoogle Scholar
15Bykov, VL.Use of low-frequency laser irradiation for the stimulation of healing of a neotympanic transplant after tympanoplasty [in Russian]. Vestn Otorinolaringol 1984;1:25–9Google Scholar
16Posten, W, Wrone, DA, Dover, JS, Arndt, KA, Silapunt, S, Alam, M.Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 2005;31:334–40CrossRefGoogle ScholarPubMed
17Baxter, GD.Therapeutic Lasers: Theory and Practice. Philadelphia: Churchill Livingstone, 1995CrossRefGoogle Scholar
18Kamrava, SK, Farhadi, M, Rezvan, F, Sharifi, D, Ashrafihellan, J, Shoaee, S et al. The histological and clinical effects of 630 nanometer and 860 nanometer low-level laser on rabbits' ear punch holes. Lasers Med Sci 2009;24:949–54CrossRefGoogle ScholarPubMed
19Stenfors, LE, Bloom, GD, Hellstrom, S.The tympanic membrane. Acta Otolaryngol 1984;414(suppl):28–30CrossRefGoogle ScholarPubMed
20Spandow, O, Hellstrom, S, Dahlstrom, M.Structural characterization of persistent tympanic membrane perforations in man. Laryngoscope 1996;106:346–52CrossRefGoogle ScholarPubMed
21Truy, E, Disant, F, Morgon, A.Chronic tympanic membrane perforation: an animal model. Am J Otol 1995;16:222–5Google ScholarPubMed
22Orji, FT, Agu, CC.Determinants of spontaneous healing in traumatic perforations of the tympanic membrane. Clin Otolaryngol 2008;33:420–6CrossRefGoogle ScholarPubMed
23Kristensen, S.Spontaneous healing of traumatic tympanic membrane perforations in man: a century of experience. J Laryngol Otol 1992;106:1037–50CrossRefGoogle Scholar
24Reeve, DR.The mitotic response of the stratified squamous epithelium at the edge of large perforations of the tympanic membrane in guinea pigs. J Anat 1977;124:731–40Google ScholarPubMed
25Medrado, AR, Pugliese, LS, Reis, SR, Andrade, ZA.Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Lasers Surg Med 2003;32:239–44CrossRefGoogle ScholarPubMed
26Lubart, R, Wollman, Y, Friedmann, H, Rochkind, S, Laulicht, I.Effects of visible and near-infrared lasers on cell cultures. J Photochem Photobiol 1992;12:305–10CrossRefGoogle ScholarPubMed
27Evans, DH, Abrahamse, H.Efficacy of three different laser wavelengths for in vitro wound healing. Photodermatol Photoimmunol Photomed 2008;24:199–210CrossRefGoogle ScholarPubMed
28Loevschall, H, Arenholt-Bindslev, D.Effect of low level diode laser irradiation on human oral mucosa fibroblasts in vitro. Lasers Surg Med 1994;14:347–54CrossRefGoogle ScholarPubMed
29Almeida-Lopes, L, Rigau, J, Zângaro, RA, Guidugli-Neto, J, Jaeger, MM.Comparison of the low level laser therapy effects on cultured human gingival fibroblast proliferation using different irradiance and same fluence. Lasers Surg Med 2001;29:179–84CrossRefGoogle ScholarPubMed
30Pereira, AN, Eduardo Cde, P, Matson, E, Marques, MM.Effect of low power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Laser Surg Med 2002;31:263–67CrossRefGoogle ScholarPubMed
31Azevedo, LH, de Paula Eduardo, F, Moreira, MS, de Paula Eduardo, C, Marques, MM.Influence of different power densities of LILT on cultured human fibroblast growth: a pilot study. Lasers Med Sci 2006;21:86–9CrossRefGoogle ScholarPubMed
32Colver, GB, Priestley, GC.Failure of helium-neon laser to affect components of wound healing in vitro. Br J Dermatol 1989;121:179–86CrossRefGoogle ScholarPubMed
33Hawkins, D, Abrahamse, H.The role of fluence in cell viability, proliferation and membrane integrity of wounded human skin fibroblasts following helium-neon laser irradiation. Lasers Surg Med 2006;38:74–83CrossRefGoogle ScholarPubMed
34Karu, TI.Molecular mechanisms of therapeutic effect of low intensity laser irradiation. Laser Life Sci 1988;2:53–74Google Scholar