Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-23T06:46:50.644Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of intratympanic mitomycin C on the development of cholesteatoma and otitis media in rats

Published online by Cambridge University Press:  14 February 2013

A A Melo ,
S S Caldas Neto ,
F S Leão  and
A J C Campos
A A Melo*
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
S S Caldas Neto
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
F S Leão
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
A J C Campos
Affiliation:
Department of Otolaryngology, Federal University of Pernambuco School of Medicine, Recife, Brazil
*
Address for correspondence: Dr Antonio Antunes Melo, Avenida José Augusto Moreira 685, Casa Caiada, CEP 53130-410, Olinda-PE, Brazil Fax: +55 8134922695 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

To determine whether the administration of mitomycin C prevents propylene glycol exposure from inducing middle-ear cholesteatoma and otitis media, in a rat model.

Methods:

Twenty-four Wistar rats underwent intratympanic injections on days 1, 8 and 15, via the tympanic membrane pars tensa, in both the right and left ears. The right ear injection solution contained 50 per cent propylene glycol, gentamicin and saline (0.9 per cent), while the left ear solution contained 50 per cent propylene glycol, gentamicin and mitomycin C. Animals were sacrificed and examined.

Results:

There were statistically significant differences between the control and experimental groups for tympanic bulla mucosal thickness (p = 0.004) but not for tympanic membrane thickness (p = 0.371), otomicroscopic findings (p = 0.262), or the presence of exudate (p = 0.125), fibrosis (p = 1.000) or cholesteatoma (p = 0.687).

Conclusion:

Intratympanic mitomycin C was ineffective in preventing middle-ear cholesteatoma and otitis media in this rat model.

Keywords

Mitomycin CCholesteatomaOtitis MediaPropylene GlycolRatsWistar
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 4 , April 2013 , pp. 359 - 363
Copyright
Copyright © JLO (1984) Limited 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Presented (and awarded a thesis prize) at the 39th Brazilian Congress of Otolaryngology and Head and Neck Surgery, 31 March to 4 April 2008, Belo Horizonte, Brazil

References

1Rüedi, L. Cholesteatoma formation in the middle ear in animal experiments. Acta Otolaryngol 1959;50:233–40CrossRefGoogle ScholarPubMed
2Hueb, MM, Goycoolea, MY, Muchow, D, Duvall, AJ, Paparella, MM, Sheridan, C. In search of missing links in otology. III. Development of a new animal model for cholesteatoma. Laryngoscope 1993;103:774–84CrossRefGoogle ScholarPubMed
3Piltcher, OB, Swarts, JD, Magnuson, K, Alper, CM, Doyle, WJ, Hebda, PA. A rat model of otitis media with effusion caused by Eustachian tube obstruction with and without Streptococcus pneumoniae infection: methods and disease course. Otolaryngol Head Neck Surg 2002;126:490–8CrossRefGoogle ScholarPubMed
4Costa, VP, Spaeth, GL, Eiferman, RA, Orengo-Nania, S. Wound healing modulation in glaucoma filtration surgery. Ophthalmic Surg 1993;24:152–70Google ScholarPubMed
5Shields, CL, Naseripour, M, Shields, JA. Topical mitomycin C for extensive, recurrent conjunctival-corneal squamous cell carcinoma. Am J Ophthalmol 2002;133:601–6CrossRefGoogle ScholarPubMed
6Daniell, M, Maini, R, Tole, D. Use of mitomycin C in the treatment of corneal conjunctival intraepithelial neoplasia. Clin Experiment Ophthalmol 2002;30:94–8CrossRefGoogle ScholarPubMed
7Larsson, C, Von Unge, M, Bagger-Sjöbäck, D. Tympanic membrane changes in experimental cholesteatoma in the gerbil. Am J Otol 1999;20:309–16Google ScholarPubMed
8Masaki, M, Wright, CG, Lee, DH, Meyerhoff, WL. Effects of otic drops on chinchilla tympanic membrane. Arch Otolaryngol Head Neck Surg 1988;114:1007–11CrossRefGoogle ScholarPubMed
9Masaki, M, Wright, CG, Lee, DH, Meyerhoff, WL. Experimental cholesteatoma: epidermal ingrowth through tympanic membrane following middle ear application of propylene glycol. Acta Otolaryngol 1989;108:113–21CrossRefGoogle ScholarPubMed
10Jassir, D, Odabasi, O, Gomez-Marin, O, Buchman, CA. Dose-response relationship of topically applied mitomycin C for the prevention of laser myringotomy closure. Otolaryngol Head Neck Surg 2003;129:471–4Google ScholarPubMed
11Santos, PF, Leal, MC, Peixoto, C, Caldas Neto, S, Rosas, STP. Otomicroscopic and histological findings of induced myringosclerosis in rats: a critical study of an experimental model. Braz J Otorhinolaryngol 2005;71:668–74CrossRefGoogle ScholarPubMed
12Antunes, ML, Fukuda, Y, Penido, NO, Ferreira, R. Effect of trans-retinoic acid in the inhibition of cholesteatoma in guinea pigs. Braz J Otorhinolaryngol 2008;74:53–60CrossRefGoogle ScholarPubMed
13Sennaroglu, L, Ozkul, A, Gedikoglu, G, Turan, E. Effect of intratympanic steroid application on the development of experimental cholesteatoma. Laryngoscope 1998;108:543–7CrossRefGoogle ScholarPubMed
14Wright, CG, Bird, LL, Meyerhoff, WL. Effect of 5-fluorouracil in cholesteatoma development in an animal model. Am J Otolaryngol 1991;12:133–8CrossRefGoogle ScholarPubMed
15Pownell, PH, Wright, CG, Robinson, KS, Meyerhoff, WL. The effect of cyclophosphamide on development of experimental cholesteatoma. Arch Otolaryngol Head Neck Surg 1994;120:1114–16CrossRefGoogle ScholarPubMed
16Wright, CG, Bird, LL, Meyerhoff, WL. Tympanic membrane microstructure in experimental cholesteatoma. Acta Otolaryngol (Stockh) 1991;111:101–11CrossRefGoogle ScholarPubMed
17Massuda, ET, Oliveira, JA. A new experimental model of acquired cholesteatoma. Laryngoscope 2005;115:481–5CrossRefGoogle ScholarPubMed
18Huang, CC, Shi, GS, Yi, ZZ. Experimental induction of middle ear cholesteatoma in rats. Am J Otolaryngol 1988;9:165–72CrossRefGoogle ScholarPubMed
19Jove, MA, Vassalli, L, Raslan, W, Applebaum, EL. The effect of isotretinoin on propylene glycol-induced cholesteatoma in chinchilla middle ears. Am J Otolaryngol 1990;11:5–9CrossRefGoogle ScholarPubMed
20White, SJ, Wright, CG, Robinson, KS, Meyerhoff, WL. Effect of topical hyaluronic acid on experimental cholesteatoma. Am J Otolaryngol 1995;16:312–18CrossRefGoogle ScholarPubMed
21Palva, T, Johnson, LG. Findings in a pair of temporal bones from a patient with secretory otitis media and chronic middle ear infection. Acta Otolaryngol 1984;98:208–20CrossRefGoogle Scholar