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Early morphological and chemical changes induced by cisplatin in the guinea pig organ of Corti

Published online by Cambridge University Press:  29 June 2007

Spiro D. Comis ,
Peter H. Rhys-Evans ,
Michel P. Osborne ,
James O. Pickles ,
David J. R. Jeffries  and
Henry A. C. Pearse
Spiro D. Comis
Affiliation:
Department of Physiology, University of Birmingham
Peter H. Rhys-Evans*
Affiliation:
Department of Otolaryngology, Queen Elizabeth Hospital, Birmingham.
Michel P. Osborne
Affiliation:
Department of Physiology, University of Birmingham
James O. Pickles
Affiliation:
Department of Physiology, University of Birmingham
David J. R. Jeffries
Affiliation:
Department of Otolaryngology, Manor Hospital, Walsall
Henry A. C. Pearse
Affiliation:
Department of Physiology, University of Birmingham
*
Mr. P. H. Rhys-Evans, ENT/Head and Neck Unit, The Royal Marsden Hospital, Fulham Road, London SW3.
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Abstract

Guinea pigs were treated with a single dose of Cisplatin (5 mg IP). After 2–4 days the cochleas were prepared for morphological analysis by scanning electron microscopy and chemical analysis by X-ray dispersive microanalysis. Following Cisplatin, the bundles of stereocilia on the hair cells were found to be rough, disarrayed, fused, and finally absorbed. Significant increases were found in the levels of calcium, sulphur, and phosporus in the abnormal hair cells. It is suggested that the high calcium levels might be due to the inhibition of enzymes which normally keep cytoplasmic calcium low, and that some of the changes in the stereocilia might be secondary to this.

Type
Research Article
Information
The Journal of Laryngology & Otology , Volume 100 , Issue 12 , December 1986 , pp. 1375 - 1383
Copyright
Copyright © JLO (1984) Limited 1986

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