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Differentiation of adipose-derived stem cells into ear auricle cartilage in rabbits

Published online by Cambridge University Press:  12 June 2012

H Bahrani ,
M Razmkhah ,
M J Ashraf ,
N Tanideh ,
N Chenari ,
B Khademi  and
A Ghaderi
H Bahrani
Affiliation:
Department of ENT, School of Medicine, Shiraz University of Medical Sciences, Iran
M Razmkhah
Affiliation:
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Iran
M J Ashraf
Affiliation:
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Iran
N Tanideh
Affiliation:
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Iran
N Chenari
Affiliation:
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Iran
B Khademi
Affiliation:
Department of ENT, School of Medicine, Shiraz University of Medical Sciences, Iran
A Ghaderi*
Affiliation:
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Iran Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Iran
*
Address for correspondence: Dr Abbas Ghaderi, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, PO Box 71345-1798, Shiraz, Iran Fax: 0098 711 230 4952 E-mail: [email protected]
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Abstract

Background:

Adipose-derived stem cells have been reported as a novel candidate for the repair of cartilage injuries in vivo.

Methods:

In order to assess their differentiation ability, adipose-derived stem cells isolated from rabbit fat tissue were injected into the midportion of a surgically created rabbit ear auricle cartilage defect. After several months, the auricles were resected, histopathologically assessed and compared with a control group.

Results:

Histopathological examination of auricles removed three, four and five months after injection showed islands of new cartilage formation at the site of the surgically induced defect. Six months after injection, we observed well-formed, mature cartilaginous plates that completely filled the defect in the native cartilage. In the control group, there was no significant growth of new cartilage.

Conclusion:

The results of this study suggest the great potential of adipose-derived stem cells to repair damaged cartilage tissue in vivo.

Keywords

Mesenchymal Stem CellsAdipose TissueCartilageRegenerative Medicine
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 8 , August 2012 , pp. 770 - 774
Copyright
Copyright © JLO (1984) Limited 2012

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