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Antioxidant effect of pomegranate extract in reducing acute inflammation due to myringotomy

Published online by Cambridge University Press:  25 February 2011

V Kahya ,
A Meric ,
M Yazici ,
M Yuksel ,
A Midi  and
O Gedikli
V Kahya
Affiliation:
Otorhinolaryngology Department, Bezmialem Vakif University Medical Faculty, Istanbul, Turkey
A Meric*
Affiliation:
Otorhinolaryngology Department, Bezmialem Vakif University Medical Faculty, Istanbul, Turkey
M Yazici
Affiliation:
Otorhinolaryngology Department, Bakirkoy Training and Research Hospital, Istanbul, Turkey
M Yuksel
Affiliation:
Biochemistry Department, Marmara University, Vocational School of Health Related Professions, Istanbul, Turkey
A Midi
Affiliation:
Pathology Department, Maltepe University Medical Faculty, Istanbul, Turkey
O Gedikli
Affiliation:
School of Health Related Professions, Kirklareli University, Kirklareli, Turkey
*
Address for correspondence: Dr Aysenur Meric, Atakoy 9, Kisim A3A Blok D:36, Bakirkoy, Istanbul, Turkey Fax: +90 212 6217580 E-mail: [email protected]
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Abstract

Objective:

To assess the effect of pomegranate extract on acute inflammation due to myringotomy.

Design:

Prospective, randomised study.

Subjects:

Thirty Sprague–Dawley rats were divided into three groups. Group one constituted controls. Group two underwent myringotomy. Group three underwent myringotomy and also received 100 µl/day pomegranate extract, via gavage, one day before and two days after surgery. Following sacrifice 48 hours after myringotomy, the animals' right ears were used to determine the concentration of reactive oxygen species, using the chemiluminescence method; left ears were used for histological study.

Results:

Reactive oxygen species levels were significantly decreased in group three compared with group two (p < 0.01). The density of inflammatory cells in group three was significantly less than that in group two (p < 0.01). Lamina propria thickness and vessel density were also significantly decreased in group three compared with group two (p < 0.01).

Conclusion:

Our results indicate that oral pomegranate extract decreases reactive oxygen species concentration and acute inflammation in the tympanic membrane after myringotomy.

Keywords

MyringotomyInflammationPathologyAntioxidantsPunicaceae
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 125 , Issue 4 , April 2011 , pp. 370 - 375
Copyright
Copyright © JLO (1984) Limited 2011

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References

1Mattsson, C, Magnuson, K, Hellström, S. Myringotomy: a prerequisite for the development of myringosclerosis. Laryngoscope 1998;108:102–6CrossRefGoogle ScholarPubMed
2Riley, DN, Herberger, S, McBride, G. Myringotomy and ventilation tube insertion: a ten year follow up. J Laryngol Otol 1997;111:257–61CrossRefGoogle ScholarPubMed
3Mattsson, C, Magnuson, K, Hellström, S. Myringosclerosis caused by increased oxygen concentration in traumatized tympanic membranes. Experimental study. Ann Otol Rhinol Laryngol 1995;104:625–32CrossRefGoogle ScholarPubMed
4Mattsson, C, Johansson, C, Hellström, S. Myringosclerosis develops within 9 hours of myringotomy. ORL J Otorhinolarygol Relat Spec 1999;61:31–6CrossRefGoogle Scholar
5Mattsson, C, Marklund, SL, Hellström, S. Application of oxygen free radical scavengers to diminish the occurrence of myringosclerosis. Ann Otol Rhinol Laryngol 1997;106:513–18CrossRefGoogle ScholarPubMed
6Polat, S, Oztürk, O, Uneri, C, Yüksel, M, Haklar, G, Bozkurt, S. Determination of reactive oxygen species in myringotomized tympanic membranes: effect of vitamin E treatment. Laryngoscope 2004;114:720–5CrossRefGoogle ScholarPubMed
7Spratley, JE, Hellström, SO, Mattsson, CK, Pais-Clemente, . Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. Ann Otol Rhinol Laryngol 2001;110:585–91CrossRefGoogle ScholarPubMed
8Akbaş, Y, Pata, YS, Görür, K, Polat, G, Polat, A, Ozcan, C. The effect of L-carnitine on the prevention of experimentally induced myringosclerosis in rats. Hear Res 2003;184:107–12CrossRefGoogle ScholarPubMed
9Ozcan, C, Gorur, K, Cinel, L, Talas, DU, Unal, M, Cinel, . The inhibitory effect of topical N- acetylcysteine application on myringosclerosis in perforated rat tympanic membrane. Int J Pediatr Otorhinolaryngol 2002;63:179–84CrossRefGoogle ScholarPubMed
10Esmaillzadeh, A, Tahbaz, F, Gaieni, I. Cholesterol-lowering effect of concentrated pomegranate juice consumption in type II diabetic patients with hyperlipidemia. Int J Vitam Nutr Res 2006;76:147–51CrossRefGoogle ScholarPubMed
11de Nigris, F, Williams-Ignarro, S, Sica, V, Lerman, LO, D'Armiento, FP, Byrns, RE et al. Effects of a pomegranate fruit extract rich in punicalagin on oxidation-sensitive genes and eNOS activity at sites of perturbed shear stress and atherogenesis. Cardiovasc Res 2007;73:414–23CrossRefGoogle Scholar
12Tugcu, V, Kemahli, E, Ozbek, E, Arinci, YV, Uhri, M, Erturkuner, P et al. Protective effect of a potent antioxidant, pomegranate juice, in the kidney of rats with nephrolithiasis induced by ethylene glycol. J Endourol 2008;22:2723–31CrossRefGoogle ScholarPubMed
13Aviram, M, Dornfeld, L. Pomegranate juice consumption inhibits serum angiotensin converting enzyme activity and reduces systolic blood pressure. Atherosclerosis 2001;158:195–8CrossRefGoogle ScholarPubMed
14Kaplan, M, Hayek, T, Raz, A, Coleman, R, Dornfeld, L, Vaya, J et al. Pomegranate juice supplementation to atherosclerotic mice reduces macrophage lipid peroxidation, cellular cholesterol accumulation and development of atherosclerosis. J Nutr 2001;131:2082–9CrossRefGoogle ScholarPubMed
15Sade, J, Luntz, M. Dynamic measurements of gas composition in the middle ear. II: steady state value. Acta Otolaryngol 1993;113:353–7CrossRefGoogle Scholar
16Parks, RR, Huang, C-C, Haddad, J. Evidence of oxygen radical injury in experimental otitis media. Laryngoscope 1994;104:1389–92CrossRefGoogle ScholarPubMed
17Mattsson, C, Hellström, S. Inhibition of the development of myringosclerosis by local administration of fenspiride, an anti-inflammatory drug. Eur Arch Otorhinolaryngol 1997;254:425–9CrossRefGoogle ScholarPubMed
18Aviram, M, Dornfeld, L, Kaplan, M, Coleman, R, Gaitini, D, Nitecki, S et al. Pomegranate juice flavonoids inhibit low-density lipoprotein oxidation and cardiovascular diseases: studies in atherosclerotic mice and in humans. Drugs Exp Clin Res 2002;28:49–62Google ScholarPubMed
19Tsuda, T, Horio, F, Osawa, T. Cyanidin 3-O-beta-D-glucoside suppresses nitric oxide production during a zymosan treatment in rats. J Nutr Sci Vitaminol (Tokyo) 2002;48:305–10CrossRefGoogle ScholarPubMed
20Görür, K, Ozcan, C, Polat, A, Unal, M, Tamer, L, Cinel, I et al. The anti-oxidant and anti-apoptotic activities of selenium in the prevention of myringosclerosis in rats. J Laryngol Otol 2002;116:426–9CrossRefGoogle ScholarPubMed
21Forséni, M, Bagger-Sjöbäck, D, Hultcrantz, M. A study of inflammatory mediators in the human tympanosclerotic middle ear. Arch Otolaryngol Head Neck Surg 2001;127:559–64CrossRefGoogle ScholarPubMed
22Kaptan, ZK, Emir, H, Gocmen, H, Uzunkulaoglu, H, Karakas, A, Senes, M et al. Ginkgo biloba, a free oxygen radical scavenger, affects inflammatory mediators to diminish the occurrence of experimental myringosclerosis. Acta Otolaryngol 2008;17:1–6Google Scholar
23Tahar Aissa, J, Hultcrantz, M. Acute tympanic membrane perforations and the early immunological response in rats. Acta Otolaryngol 2009;23:1–6CrossRefGoogle Scholar
24Schiff, M, Poliquin, JF, Cantanzaro, A. Tympanosclerosis: a theory of pathogenesis. Ann Otol Rhinol Laryngol Suppl 1980;89:16–28Google ScholarPubMed
25Hellström, S, Spratley, J, Eriksson, PO, Pais-Clemente, M. Tympanic membrane vessel revisited: a study in an animal model. Otol Neurotol 2003;24:494–9CrossRefGoogle ScholarPubMed