Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-09T07:22:00.953Z Has data issue: false hasContentIssue false
  • English
  • Français

Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery

Published online by Cambridge University Press:  15 August 2012

B Levin ,
S L Redmond ,
R Rajkhowa ,
R H Eikelboom ,
M D Atlas  and
R J Marano
B Levin*
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia Sir Charles Gairdner Hospital, Perth, Western Australia, Australia
S L Redmond
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia
R Rajkhowa
Affiliation:
Centre for Material and Fibre Innovation, Deakin University, Geelong, Victoria, Australia
R H Eikelboom
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia
M D Atlas
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia Sir Charles Gairdner Hospital, Perth, Western Australia, Australia St John of God Hospital, Perth, Western Australia, Australia
R J Marano
Affiliation:
Ear Science Institute Australia, Perth, Western Australia, Australia Ear Sciences Centre, School of Surgery, The University of Western Australia, Perth, Western Australia, Australia
*
Address for correspondence: Dr Brett Levin, Ear Sciences Centre, School of Surgery, Suite 3, 1 Salvado Rd, Subiaco, WA, 6008 Fax: +61 (02) 9845 9852 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Background:

Chronic tympanic membrane perforations can cause significant morbidity. The term myringoplasty describes the operation used to close such perforations. A variety of graft materials are available for use in myringoplasty, but all have limitations and few studies report post-operative hearing outcomes. Recently, the biomedical applications of silk fibroin protein have been studied. This material's biocompatibility, biodegradability and ability to act as a scaffold to support cell growth prompted an investigation of its interaction with human tympanic membrane keratinocytes.

Methods and materials:

Silk fibroin membranes were prepared and human tympanic membrane keratinocytes cultured. Keratinocytes were seeded onto the membranes and immunostained for a number of relevant protein markers relating to cell proliferation, adhesion and specific epithelial differentiation.

Results:

The silk fibroin scaffolds successfully supported the growth and adhesion of keratinocytes, whilst also maintaining their cell lineage.

Conclusion:

The properties of silk fibroin make it an attractive option for further research, as a potential alternative graft in myringoplasty.

Keywords

Tympanic Membrane PerforationMyringoplastySilk FibroinGraftKeratinocyte
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Supplement S1 , January 2013 , pp. S13 - S20
Copyright
Copyright © JLO (1984) Limited 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Freid, VM, Makuc, DM, Rooks, RN. Ambulatory health care visits by children: principal diagnosis and place of visit. Vital Health Stat 13 1998;May:1–23Google ScholarPubMed
2Silveira Netto, LF, da Costa, SS, Sleifer, P, Braga, ME. The impact of chronic suppurative otitis media on children's and teenagers' hearing. Int J Pediatr Otorhinolaryngol 2009;73:1751–6CrossRefGoogle ScholarPubMed
3Bluestone, CD. Epidemiology and pathogenesis of chronic suppurative otitis media: implications for prevention and treatment. Int J Pediatr Otorhinolaryngol 1998;42:207–23CrossRefGoogle ScholarPubMed
4Verhoeff, M, van der Veen, EL, Rovers, MM, Sanders, EA, Schilder, AG. Chronic suppurative otitis media: a review. Int J Pediatr Otorhinolaryngol 2006;70:1–12CrossRefGoogle ScholarPubMed
5Nittrouer, S, Burton, LT. The role of early language experience in the development of speech perception and phonological processing abilities: evidence from 5-year-olds with histories of otitis media with effusion and low socioeconomic status. J Commun Disord 2005;38:29–63CrossRefGoogle ScholarPubMed
6Matsuda, Y, Kurita, T, Ueda, Y, Ito, S, Nakashima, T. Effect of tympanic membrane perforation on middle-ear sound transmission. J Laryngol Otol 2009;123(suppl):81–9CrossRefGoogle Scholar
7Kangsanarak, J, Fooanant, S, Ruckphaopunt, K, Navacharoen, N, Teotrakul, S. Extracranial and intracranial complications of suppurative otitis media. Report of 102 cases. J Laryngol Otol 1993;107:999–1004CrossRefGoogle ScholarPubMed
8Lin, YS, Lin, LC, Lee, FP, Lee, KJ. The prevalence of chronic otitis media and its complication rates in teenagers and adult patients. Otolaryngol Head Neck Surg 2009;140:165–70CrossRefGoogle ScholarPubMed
9Levin, B, Rajkhowa, R, Redmond, SL, Atlas, MD. Grafts in myringoplasty: utilizing a silk fibroin scaffold as a novel device. Expert Rev Med Devices 2009;6:653–64CrossRefGoogle ScholarPubMed
10Vartiainen, E, Nuutinen, J. Success and pitfalls in myringoplasty: follow-up study of 404 cases. Am J Otol 1993;14:301–5Google ScholarPubMed
11Yuen, AP, Ho, WK, Hui, Y, Wei, WI, Au, DK. Correlation of pure tone audiogram results and hearing benefit of tympanoplasty for chronic suppurative otitis media. Ann Otol Rhinol Laryngol 2000;109:381–4CrossRefGoogle ScholarPubMed
12Nagai, T, Tono, T. Encapsulated nerve corpuscles in the human tympanic membrane. Arch Otorhinolaryngol 1989;246:169–72CrossRefGoogle ScholarPubMed
13Teoh, SW, Flandermeyer, DT, Rosowski, JJ. Effects of pars flaccida on sound conduction in ears of Mongolian gerbil: acoustic and anatomical measurements. Hear Res 1997;106:39–65CrossRefGoogle ScholarPubMed
14Dirckx, JJ, Decraemer, WF, von Unge, M, Larsson, C. Volume displacement of the gerbil eardrum pars flaccida as a function of middle ear pressure. Hear Res 1998;118:35–46CrossRefGoogle ScholarPubMed
15Aggarwal, R, Saeed, SR, Green, KJ. Myringoplasty. J Laryngol Otol 2006;120:429–32CrossRefGoogle ScholarPubMed
16Yung, M. Cartilage tympanoplasty: literature review. J Laryngol Otol 2008;122:663–72CrossRefGoogle ScholarPubMed
17Dursun, E, Dogru, S, Gungor, A, Cincik, H, Poyrazoglu, E, Ozdemir, T et al. Comparison of paper-patch, fat, and perichondrium myringoplasty in repair of small tympanic membrane perforations. Otolaryngol Head Neck Surg 2008;138:353–6CrossRefGoogle ScholarPubMed
18Saadat, D, Ng, M, Vadapalli, S, Sinha, UK. Office myringoplasty with alloderm. Laryngoscope 2001;111:181–4CrossRefGoogle ScholarPubMed
19Schulte, DL, Driscoll, CL, McDonald, TJ, Facer, GW, Beatty, CW. Irradiated rib cartilage graft for reconstruction of the tympanic membrane: preliminary results. Am J Otol 1998;19:141–4Google ScholarPubMed
20Kaftan, H, Noack, M, Friedrich, N, Volzke, H, Hosemann, W. Prevalence of chronic tympanic membrane perforation in the adult population [in German]. HNO 2008;56:145–50CrossRefGoogle ScholarPubMed
21Wang, Y, Kim, HJ, Vunjak-Novakovic, G, Kaplan, DL. Stem cell-based tissue engineering with silk biomaterials. Biomaterials 2006;27:6064–82CrossRefGoogle ScholarPubMed
22Altman, GH, Diaz, F, Jakuba, C, Calabro, T, Horan, RL, Chen, J et al. Silk-based biomaterials. Biomaterials 2003;24:401–16CrossRefGoogle ScholarPubMed
23Wang, Y, Rudym, DD, Walsh, A, Abrahamsen, L, Kim, HJ, Kim, HS et al. In vivo degradation of three-dimensional silk fibroin scaffolds. Biomaterials 2008;29:3415–28CrossRefGoogle ScholarPubMed
24Amoils, CP, Jackler, RK, Milczuk, H, Kelly, KE, Cao, K. An animal model of chronic tympanic membrane perforation. Otolaryngol Head Neck Surg 1992;106:47–55CrossRefGoogle Scholar
25Kaftan, H, Hosemann, W, Beule, A, Junghans, D. An improved animal model for chronic perforation of the tympanic membrane [in German]. HNO 2004;52:714–19Google ScholarPubMed
26Santa Maria, PL, Atlas, MD, Ghassemifar, R. Chronic tympanic membrane perforation: a better animal model is needed. Wound Repair Regen 2007;15:450–8CrossRefGoogle ScholarPubMed
27Levin, B, Redmond, SL, Rajkhowa, R, Eikelboom, RH, Marano, RJ, Atlas, MD et al. Preliminary results of the application of a silk fibroin scaffold to otology. Otolaryngol Head Neck Surg 2010;142(suppl 1):S33–5CrossRefGoogle ScholarPubMed
28Ghassemifar, R, Redmond, S, Zainuddin, Chirila, TV. Advancing towards a tissue-engineered tympanic membrane: silk fibroin as a substratum for growing human eardrum keratinocytes. J Biomater Appl 2010;24:591–606CrossRefGoogle ScholarPubMed
29Vorwerk, U, Hey, M, Steinicke, G, Begall, K. High-speed digital videoimaging of fast eardrum motions during Valsalva maneuver. ORL J Otorhinolaryngol Relat Spec 1998;60:138–42CrossRefGoogle ScholarPubMed
30Stenfors, LE, Bloom, GD, Hellstrom, S. The tympanic membrane. Acta Otolaryngol Suppl (Stockh) 1984;414:28–30CrossRefGoogle ScholarPubMed
31Stenfeldt, K, Johansson, C, Hellstrom, S. The collagen structure of the tympanic membrane: collagen types I, II, and III in the healthy tympanic membrane, during healing of a perforation, and during infection. Arch Otolaryngol Head Neck Surg 2006;132:293–8CrossRefGoogle ScholarPubMed
32Redmond, SL, Levin, B, Heel, KA, Atlas, MD, Marano, RJ. Phenotypic and genotypic profile of human tympanic membrane derived cultured cells. J Molec Hist 2011;42:15–25CrossRefGoogle ScholarPubMed
33Oettgen, P, Alani, RM, Barcinski, MA, Brown, L, Akbarali, Y, Boltax, J et al. Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family. Mol Cell Biol 1997;17:4419–33CrossRefGoogle ScholarPubMed
34Fanning, AS, Jameson, BJ, Jesaitis, LA, Anderson, JM. The tight junction protein zona occludens protein 1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton. J Biol Chem 1998;273:29745–53CrossRefGoogle Scholar
35Furuse, M, Itoh, M, Hirase, T, Nagafuchi, A, Yonemura, S, Tsukita, S. Direct association of occludin with zona occludens protein 1 and its possible involvement in the localization of occludin at tight junctions. J Cell Biol 1994;127:1617–26CrossRefGoogle Scholar
36Cepek, KL, Shaw, SK, Parker, CM, Russell, GJ, Morrow, JS, Rimm, DL et al. Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin. Nature 1994;372:190–3CrossRefGoogle Scholar
37Adams, CL, Chen, YT, Smith, SJ, Nelson, WJ. Mechanisms of epithelial cell-cell adhesion and cell compaction revealed by high-resolution tracking of E-cadherin-green fluorescent protein. J Cell Biol 1998;142:1105–19CrossRefGoogle ScholarPubMed
38Hazan, C, Melzer, K, Panageas, KS, Li, E, Kamino, H, Kopf, A et al. Evaluation of the proliferation marker MIB-1 in the prognosis of cutaneous malignant melanoma. Cancer 2002;95:634–40CrossRefGoogle ScholarPubMed