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S-100 protein localization in minor salivary gland tumours: an aid to diagnosis

Published online by Cambridge University Press:  29 June 2007

J. B. Campbell ,
J Crocker  and
P. M. Shenoi
J. B. Campbell*
Affiliation:
Department of Otolaryngology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
J Crocker
Affiliation:
Department of Pathology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
P. M. Shenoi
Affiliation:
Department of Otolaryngology, East Birmingham Hospital, Bordesley Green East, Birmingham B9 5ST.
*
Department of Otolaryngology, The Queen Elizabeth Hospital, Queen Elizabeth Medical Centre, Edgbaston, Birmingham B15 2TH.
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Abstract

The clinician is almost entirely dependent on the histopathologist to accurately diagnose minor salivary gland tumours, but in some cases the histological interpretation of the specimen is very difficult. Recently it has been demonstrated using immunohistochemical techniques that S-100 protein is present in some salivary gland tissues and its localization has been used as an aid in the differentiation of major salivary gland tumours. To assess its value in the diagnosis of minor salivary gland tumours it was localized in sections from 15 such tumours using both a standard peroxidase-antiperoxidase (PAP) and a newly developed immunogold-silver staining sequence (IGSS) technique. Strong staining for S-100 protein was seen in the nuclei and cytoplasm of the cellular areas and also in the cells in the chondroid and myxoid areas of pleomorphic adenomas. Generally the staining was more intense and widespread with the IGSS method. No staining was observed in any of the other tumour types. We conclude that S-100 protein localization is a valuable aid in the differentiation of minor salivary gland tumours. Furthermore, the IGSS method enables more sensitive ‘reading’ of the staining reaction.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 102 , Issue 10 , October 1988 , pp. 905 - 908
Copyright
Copyright © JLO (1984) Limited 1988

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