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Neuroendocrine carcinoma of the lip (Merkel Cell tumour) examined by electron microscopy and immunohistochemistry

Published online by Cambridge University Press:  29 June 2007

M. Boysen ,
P. Wetteland ,
T. Hovig  and
P. Brandtzaeg
M. Boysen*
Affiliation:
Departments of Otolaryngology, Institute of Pathology, University of Oslo, The National Hospital, (Rikshospitalet), N-0027, Oslo 1, Norway.
P. Wetteland
Affiliation:
Departments of Pathology, Institute of Pathology, University of Oslo, The National Hospital, (Rikshospitalet), N-0027, Oslo 1, Norway.
T. Hovig
Affiliation:
Laboratory for Electron MicroscopyInstitute of Pathology, University of Oslo, The National Hospital, (Rikshospitalet), N-0027, Oslo 1, Norway.
P. Brandtzaeg
Affiliation:
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo, The National Hospital, (Rikshospitalet), N-0027, Oslo 1, Norway.
*
M. Boysen, M.D., Department of Otolaryngology, Rikshospitalet, N-0027 Oslo 1, Norway.
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Abstract

A definite diagnosis of neuroendocrine carcinoma of the skin is seldom made on initial histological examination; the tumour is usually reported as a poorly differentiated or anaplastic carcinoma. By applying electron microscopy and immunohistochemistry, a correct diagnosis can be made. The ultrastructural examination shows dense-core membrane-bound granules, intermediate perinuclear filaments and desmosome-like junctions. Immunohistochemistry reveals positive staining for neuron-specific enolase and keratin, the latter in a characteristic paranuclear distribution. Confronted with an unusual clinical picture or indefinite histological diagnosis, tissue specimens should be secured for the above mentioned ancillary procedures.

Type
Clinical Records
Information
The Journal of Laryngology & Otology , Volume 103 , Issue 5 , May 1989 , pp. 519 - 523
Copyright
Copyright © JLO (1984) Limited 1989

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References

Battifora, H. and Silva, E. G. (1986) The use of antikeratin antibodies in the immunohistochemical distinction between neuroendocrine (Merkel cell) carcinoma of the skin, lymphoma and oat cell carcinoma. Cancer, 58: 1040–1046.3.0.CO;2-L>CrossRefGoogle ScholarPubMed
Brandtzaeg, P. (1974) Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with cold eth-anol-fixation technique. Immunology, 26: 1101–1113.Google ScholarPubMed
Brandtzaeg, P. and Rognum, T. O. (1983) Evaluation of tissue preparation methods and paired immunofluorescence staining for immunocytochemistry of lymphomas. Histochepxical Journal, 15: 655–689.CrossRefGoogle ScholarPubMed
Brandtzaeg, P., Dale, I. and Fagerhol, M. K. (1987) Distribution of a formalin-resistant myelomonocytic antigen (LI) in human tissues. 1. Comparison with other leukocyte markers by paired immunofluorescence and immunoenzyme staining. American Journal of Clinical Pathology, 87: 681–699.CrossRefGoogle Scholar
De Wolf-Peeters, C., Marien, K.., Mebis, J. and Desmet, V. (1980) A cutaneous APUDoma or Merkel cell tumour? A morphologically recognizable tumour with a biological and histological malignant aspect in contrast with its clinical behaviour. Cancer, 46: 1810–1816.3.0.CO;2-7>CrossRefGoogle Scholar
Drijkoningen, M., de Wolf-Peeters, C, van Limbergen, E. and Desmet, V. (1986) Merkel cell tumor of the skin: An immunohistochemical study. Human Pathology, 17: 301–310.CrossRefGoogle ScholarPubMed
Ferlito, A. (1986) Diagnosis and treatment of small cell carcinoma of the larynx: A critical review. Annals of Otology, Rhinology and Laryngology, 95: 590–600.CrossRefGoogle ScholarPubMed
Gomez, L. G., DiMaio, S., Silva, E. G. and Mackay, B. (1983) Association between neuroendocrine (Merkel cell) carcinoma and squamous carcinoma of the skin. American Journal of Surgical Pathology, 7: 171–177.CrossRefGoogle ScholarPubMed
Gould, V. E., Moll, R., Moll, I., Lee, I. and Franke, W. W. (1985) Biology of disease. Neuroendocrine (Merkel) cells of the skin: Hyperplasias, dysplasias and neoplasms. Laboratory Investigation, 52: 334–353.Google Scholar
Hall, P. A., D'Ardenne, A. J., Butler, M. G., Stearn, P. M., Subbuswamy, S. G., Blackshaw, A. J. and Levison, D. A. (1986) Cytokeratin and laminin immunostaining in the diagnosis of cutaneous neuroendocrine (Merkel cell) tumours. Histopathology, 10: 1179–1190.CrossRefGoogle ScholarPubMed
Huidtfeldt, H. S. and Brandtzaeg, P. (1985) Various keratin antibodies produce immunohistochemical staining of human myocardium and myometrium. Histochemistry, 83: 381–389.CrossRefGoogle Scholar
Ibrahim, N. B. N., Briggs, J. C. and Corbishley, C. M. (1984) Extrapulmonary oat cell carcinoma. Cancer, 54: 1645–1661.3.0.CO;2-Q>CrossRefGoogle ScholarPubMed
Iwasaki, H., Mitsui, T., Kikuchi, M., Imai, T. and Kukushima, K. (1981) Neuroendocrine carcinoma (trabecular carcinoma) of the skin with ectopic ACTH production. Cancer, 48: 753–756.3.0.CO;2-9>CrossRefGoogle ScholarPubMed
Johannessen, J. V. and Gould, V. E. (1980) Neuroendocrine skin carcinoma associated with calcitonin production: A Merkel cell carcinoma? Human Pathology, Supplement 5: 586–589.Google Scholar
Kameya, T., Shimosato, Y., Adachi, I., Abe, K., Ebihara, S. and Oni, I. (1980) Neuroendocrine carcinoma of the paranasal sinus. A morphological and endocrinological study. Cancer, 45: 330–339.3.0.CO;2-S>CrossRefGoogle ScholarPubMed
Kraemer, B. B. G., Mackay, B. and Batsakis, J. G. (1983) Small cell carcinoma of the parotid gland. A clinicopathologic study of three cases. Cancer, 52: 2115–2121.3.0.CO;2-Z>CrossRefGoogle ScholarPubMed
Kuhajda, F. P., Olson, J. L. and Mann, R. B. (1986) Merkel cell (small cell) carcinoma of the skin: immunohistochemical and ultrastructural demonstration of distinctive perinuclear cytokeratin aggregates and a possible association with B cell neoplasms. Histochemical Journal, 18: 239–244.CrossRefGoogle Scholar
Leong, A. S-Y., Phillips, G. E., Pieterse, A. S. and Milios, J. (1986) Criteria for the diagnoses of primary endocrine carcinoma of the skin (Merkel cell carcinoma). A histological, immunohistochemical and ultrastructural study of 13 cases. Pathology, 18: 393–399.CrossRefGoogle ScholarPubMed
Leong, A. S-Y. and Milios, J. (1986) Cytokeratin distribution in Merkel cell tumor of the skin. Human Pathology, 18: 308–309.CrossRefGoogle Scholar
Moya, C. E., Guarda, L. A., Dyer, G. A., Silva, E. G. and Shah, S. (1982) Neuroendocrine carcinoma of the skin in a young adult. American Journal of Clinical Pathology, 78: 793–785.CrossRefGoogle ScholarPubMed
Pearse, A. G. E. (1974) The APUD cell concept and its implications in pathology. Pathological Annates, 9: 27–29.Google Scholar
Reid, H. A. S., Richardson, W. W. and Corrin, B. (1980) Oat cell carcinoma of the esophagus. Cancer, 45: 2342–2347.3.0.CO;2-E>CrossRefGoogle ScholarPubMed
Sibley, R. K., Dehner, L. P. and Rosai, J. (1985) Primary neuroendocrine (Merkel cell?) carcinoma of the skin. I. A clinicopathologic and ultrastructural study of 43 cases. American Journal of Surgical Pathology, 9: 95–108.CrossRefGoogle Scholar
Tang, C-K. and Toker, C. (1978) Trabecular carcinoma of the skin. An ultrastructural study. Cancer, 42: 2311–2321.3.0.CO;2-L>CrossRefGoogle ScholarPubMed
Toker, C. (1978) Trabecular carcinoma of the skin. Archives of Dermatology, 105: 107–110.CrossRefGoogle Scholar
Warner, T. F. C. S., Uno, H., Hafez, G. R., Burgess, J., Bolles, C, Lloyd, R. V., Oka, M. (1983) Merkel cells and Merkel cell tumours. Ultrastructure, immunocytochemistry and review of the literature. Cancer, 52: 238–245.3.0.CO;2-W>CrossRefGoogle ScholarPubMed
Wick, M. R., Goellner, J. R., Scheithauer, B. W., Thomas, J. R., Sanchez, N. P. and Schroeter, A. L. (1983) Primary neuroendocrine carcinomas of the skin (Merkel cell tumors). American Journal of Clinical Pathology, 79: 6–13.CrossRefGoogle ScholarPubMed