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Morphological assessment of the luminal surface of olfactory epithelium in mice deficient in tissue plasminogen activator following bulbectomy

Published online by Cambridge University Press:  19 September 2012

N Makino ,
S Ookawara ,
S Madoiwa ,
Y Ohta ,
T Ishikawa ,
K Katoh ,
S Takigami ,
T Kanazawa ,
O Matsuo  and
M Ichikawa
...Show all authors
N Makino*
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
S Ookawara
Affiliation:
Department of Anatomy, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
S Madoiwa
Affiliation:
Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
Y Ohta
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
T Ishikawa
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
K Katoh
Affiliation:
Department of Anatomy, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
S Takigami
Affiliation:
Laboratory of Anatomy and Cellular Biology, Department of Medical Technology, Faculty of Health Sciences, Kyorin University, Hatioji, Tokyo, Japan
T Kanazawa
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
O Matsuo
Affiliation:
Department of Physiology IIy, Kinki University School of Medicine, Osakasayama City, Japan
M Ichikawa
Affiliation:
Laboratory of Anatomy and Cell Biology, Department of Neuroscience Basic Technology, Tokyo Metropolitan Institute for Neuroscienceo, Fuchu, Toky, Japan
J Mimuro
Affiliation:
Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
Y Sakata
Affiliation:
Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
K Ichimura
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
*
Address for correspondence: Dr Nobuko Makino, Department of Otolaryngology – Head and Neck Surgery, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan Fax: +81 285 44 5547 E-mail: [email protected]
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Abstract

Objective:

This study aimed to investigate the function of tissue plasminogen activator in the olfactory epithelium of mice following neural injury.

Method:

Transmission electron microscopy was used to study the changes in the morphology of the olfactory epithelium 1–7 days after surgical ablation of the olfactory bulb (bulbectomy).

Results:

Prior to bulbectomy, a uniformly fine material was observed within some regions of the olfactory epithelium of mice deficient in tissue plasminogen activator. At 2–3 days after bulbectomy, there were degenerative changes in the olfactory epithelium. At 5–7 days after bulbectomy, we noted drastic differences in olfactory epithelium morphology between mice deficient in tissue plasminogen activator and wild-type mice (comparisons were made using findings from a previous study). The microvilli seemed to be normal and olfactory vesicles and receptor neuron dendrites were largely intact in the olfactory epithelium of mice deficient in tissue plasminogen activator.

Conclusion:

The tissue plasminogen activator plasmin system may inhibit the regeneration of the olfactory epithelium in the early stages following neural injury.

Keywords

MouseMicroscopyElectronTransmissionPlasminogen ActivatorsOlfactionPathophysiologyRegeneration
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 11 , November 2012 , pp. 1114 - 1120
Copyright
Copyright © JLO (1984) Limited 2012

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References

1 Graziadei, PP, Graziadei, GA. Neurogenesis and neuron regeneration in the olfactory system of mammals. I. Morphological aspects of differentiation and structural organization of the olfactory sensory neurons. J Neurocytol 1979;8:1–18 CrossRefGoogle ScholarPubMed
2 Suzuki, Y. Fine structural aspects of apoptosis in the olfactory epithelium. J Neurocytol 2004;33:693–702 CrossRefGoogle ScholarPubMed
3 Makino, N, Ookawara, S, Katoh, K, Ohta, Y, Ichikawa, M, Ichimura, K. The morphological change of supporting cells in the olfactory epithelium after bulbectomy. Chem Senses 2009;34:171–9CrossRefGoogle ScholarPubMed
4 Nagai, N, Matsuo, O. Roles of fibrinolytic system components in the nervous system. Pathophysiology 2010;17:141–7CrossRefGoogle ScholarPubMed
5 Siao, CJ, Fernandez, SR, Tsirka, SE. Cell type-specific roles for tissue plasminogen activator released by neurons or microglia after excitotoxic injury. J Neurosci 2003;23:3234–42CrossRefGoogle ScholarPubMed
6 Nagai, N, Yamamoto, S, Tsuboi, T, Ihara, H, Urano, T, Takada, Y et al. Tissue-type plasminogen activator is involved in the process of neuronal death induced by oxygen-glucose deprivation in culture. J Cereb Blood Flow Metab 2001;21:631–4CrossRefGoogle ScholarPubMed
7 Lee, SR, Lok, J, Rosell, A, Kim, HY, Murata, Y, Atochin, D et al. Reduction of hippocampal cell death and proteolytic responses in tissue plasminogen activator knockout mice after transient global cerebral ischemia. Neuroscience 2007;150:50–7CrossRefGoogle ScholarPubMed
8 Abe, Y, Nakamura, H, Yoshino, O, Oya, T, Kimura, T. Decreased neural damage after spinal cord injury in tPA-deficient mice. J Neurotrauma 2003;20:43–57 CrossRefGoogle ScholarPubMed
9 Thewke, DP, Seeds, NW. Expression of hepatocyte growth factor/scatter factor, its receptor c-met, and tissue-type plasminogen activator during development of the murine olfactory system. J Neurosci 1996;16:6933–44CrossRefGoogle ScholarPubMed
10 Carmeliet, P, Schoonjans, L, Kieckens, L, Ream, B, Degen, J, Bronson, R et al. Physiological consequences of loss of plasminogen activator gene function in mice. Nature 1994;368:419–24CrossRefGoogle ScholarPubMed
11 Nicole, O, Docagne, F, Ali, C, Margaill, I, Carmeliet, P, MacKenzie, ET et al. The proteolytic activity of tissue-plasminogen activator enhances NMDA receptor-mediated signaling. Nat Med 2001;7:59–64 CrossRefGoogle ScholarPubMed
12 Gravanis, I, Tsirka, SE. Tissue plasminogen activator and glial function. Glia 2005;49:177–83CrossRefGoogle ScholarPubMed
13 Tsuji, K, Aoki, T, Tejima, E, Arai, K, Lee, SR, Atochin, DN et al. Tissue plasminogen activator promotes matrix metalloproteinase-9 upregulation after focal cerebral ischemia. Stroke 2005;36:1954–9CrossRefGoogle ScholarPubMed
14 Jang, W, Kim, KP, Schwob, JE. Nonintegrin laminin receptor precursor protein is expressed on olfactory stem and progenitor cells. J Comp Neurol 2007;502:367–81CrossRefGoogle ScholarPubMed
15 Costanzo, RM, Perrino, LA, Kobayashi, M. Response of matrix metalloproteinase-9 to olfactory nerve injury. Neuroreport 2006;17:1787–91CrossRefGoogle ScholarPubMed
16 Tsukatani, T, Fillmore, HL, Hamilton, HR, Holbrook, EH, Costanzo, RM. Matrix metalloproteinase expression in the olfactory epithelium. Neuroreport 2003;14:1135–40CrossRefGoogle ScholarPubMed
17 Costanzo, RM, Perrino, LA. Peak in matrix metaloproteinases-2 levels observed during recovery from olfactory nerve injury. Neuroreport 2008;19:327–31CrossRefGoogle ScholarPubMed
18 Bakos, SR, Schwob, JE, Costanzo, RM. Matrix metalloproteinase-9 and -2 expression in the olfactory bulb following methyl bromide gas exposure. Chem Senses 2010;35:655–61CrossRefGoogle ScholarPubMed
19 Llevadot, J, Giugliano, RP, Antman, EM. Bolus fibrinolytic therapy in acute myocardial infarction. JAMA 2001;286:442–9CrossRefGoogle ScholarPubMed
20 Fugate, JE, Giraldo, EA, Rabinstein, AA. Thrombolysis for cerebral ischemia. Front Neurol 2010;1:139CrossRefGoogle ScholarPubMed