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In vitro induction and differentiation of newborn guinea pig hippocampus neural stem cells into cells resembling inner hair cells, using artificial perilymph

Published online by Cambridge University Press:  07 June 2011

Y Wang  and
M-M Dong
Y Wang
Affiliation:
Department of Otolaryngology, First Affiliated Hospital of Zhengzhou University, China
M-M Dong*
Affiliation:
Department of Otolaryngology, First Affiliated Hospital of Zhengzhou University, China
*
Address for correspondence: Dr Ming-Min Dong, 1 Constructive East Road, Zhengzhou City, China 450052 Fax: +86 024 23380681 E-mail: [email protected]
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Abstract

Objective:

To investigate whether artificial perilymph can induce neural stem cells, derived from the hippocampus of newborn guinea pigs, to differentiate into inner ear hair cells, in vitro.

Methods:

Primary neural stem cells derived from the hippocampus of newborn guinea pigs were incubated in medium containing either 10 per cent fetal bovine serum or 5, 10 or 15 per cent artificial perilymph, for three weeks. Differentiated cells were identified using immunofluorescence, Western blot and scanning electron microscopy.

Results:

Both fetal bovine serum and artificial perilymph induced the neural stem cells to differentiate into cells with hair-cell-specific antibodies.

Conclusion:

Neural stem cells can survive in both fetal bovine serum and artificial perilymph, and within these media can differentiate into cells with hair-cell-specific antibodies. This provides an experimental basis for transplantation of neural stem cells into the inner ear.

Keywords

Ear, InnerStem CellsHair Cells, AuditoryCell DifferentiationPerilymphGuinea Pig
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 125 , Issue 8 , August 2011 , pp. 771 - 775
Copyright
Copyright © JLO (1984) Limited 2011

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References

1Clarke, DL, Johansson, CB, Wilbertz, J, Veress, B, Nilsson, E, Karlstrom, H et al. Generalized potential of adult neural stem cells. Science 2000;288:1660–3CrossRefGoogle ScholarPubMed
2Wang, Yun-fang, Pei, Xue-tao. Adult stem cells and their application in regenerative medicine. Chin Sci Bull 2003;48:750–4Google Scholar
3Bian, Lin, Hui, Guo-zhen, Lu, Hua, Jiang, Yun-zhao, Miao, Zong-ning, Wang, Ling et al. The experiment on the normal adult cerebrospinal fluid induction of fetal brain derived neural stem cells. Journal of Jiangsu Clinical Medicine 2003;7:202–4Google Scholar
4Kojima, K, Tamura, S, Nishida, TA, Ito, J. Generation of inner ear hair cell immunophenotypes from neurospheres obtained from fetal rat central nervous system in vitro. Acta Otolaryngol Suppl 2004;551:26–30CrossRefGoogle Scholar
5Su, Zhen-lun, Jiang, Si-chang, Gu, Rui, Yang, Wei-ping. Calcium channels in bullfrog saccular hair cells [in Chinese]. Zhonghua Er Bi Yan Hou Ke Za Zhi 1992;27:133–35Google Scholar
6Xi, Kai, Dong, Ming-min. Identification and culturing of neural stem cells from guinea pig hippocampi tissue. Journal of Zhengzhou University (Science Medical) 2005;40:471–3Google Scholar
7Shen, Xue-li, Jia, Li-hong, Shi, Yu-xiu. Study on nerve stem cell orientation differentiation to cholinergic neuron. China Journal of Modern Medicine 2005;15:520–3Google Scholar
8Westelund, U, Morten, C, Varghese, M, Berg-Johnsen, J, Ohisson, M, Langmoen, IA et al. Stem cells from the adult human brain develop into functional neurons in culture. Exp Cell Res 2003;289:378–83CrossRefGoogle Scholar
9Ito, J. Regeneration of the auditory pathway [in Japanese]. Nippon Rinsho 2003;61:469–74Google ScholarPubMed
10Fujino, K, Kim, TS, Nishida, AT, Nakagawa, T, Omori, K, Naito, Y et al. Transplantation of neural stem cells into explants of rat inner ear. Acta Otolaryngol Suppl 2004;551:31–3CrossRefGoogle Scholar
11Punnett, A, Bliss, B, Dupuis, LL, Abdolell, M, Doyle, J, Sung, L. Ototoxicity following pediatric hematopoietic stem cell transplantation: a prospective cohort study. Pediatr Blood Cancer 2004;42:598–603CrossRefGoogle ScholarPubMed