Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-27T03:43:00.284Z Has data issue: false hasContentIssue false
  • English
  • Français

Histone acetylation in refractory sudden sensorineural hearing loss patients after intratympanic methylprednisolone perfusion

Published online by Cambridge University Press:  11 September 2019

L Xie ,
J Hou ,
H Qi ,
Y Dai  and
W She
L Xie
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, China Department of Otolaryngology, Children's Hospital Affiliated to Nanjing Medical University, China
J Hou
Affiliation:
Otorhinolaryngology Research Institute of Nanjing Drum Tower Hospital, China Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline, China
H Qi
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline, China
Y Dai
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, China Otorhinolaryngology Research Institute of Nanjing Drum Tower Hospital, China Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline, China
W She*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, China Otorhinolaryngology Research Institute of Nanjing Drum Tower Hospital, China Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical Discipline, China
*
Author for correspondence: Dr Wandong She, Department of Otolaryngology – Head and Neck Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing 210008, China E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective

To examine the relationship between the therapeutic effect of intratympanic methylprednisolone perfusion and histone acetylation in refractory sudden sensorineural hearing loss.

Methods

Thirty-four refractory sudden sensorineural hearing loss patients were enrolled and treated with intratympanic methylprednisolone perfusion. Pure tone average, acetylated histone H3, acetylated histone H4 and histone deacetylase 2 (HDAC2) were measured in peripheral blood mononuclear cells before and after intratympanic methylprednisolone perfusion. Sixteen healthy volunteers were recruited to obtain normal reference values.

Results

Pure tone average in sudden sensorineural hearing loss patients improved from 84.14 ± 13.54 dB to 73.56 ± 18.45 dB after intratympanic methylprednisolone perfusion. Up-regulations in HDAC2 protein level, and down-regulations in histone H3 and H4 acetylation were observed in the intratympanic methylprednisolone perfusion sensitive group (pure tone average gain of 15 dB or more), while no significant changes were observed in the intratympanic methylprednisolone perfusion insensitive group (pure tone average gain of less than 15 dB).

Conclusion

Intratympanic methylprednisolone perfusion can improve hearing in a considerable number of refractory sudden sensorineural hearing loss patients. The therapeutic effect is closely related to reduced histone acetylation.

Keywords

MethylprednisoloneHistoneHistone Deacetylase 2Hearing LossPrognosis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 133 , Issue 10 , October 2019 , pp. 895 - 902
Copyright
Copyright © JLO (1984) Limited, 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Dr W She takes responsibility for the integrity of the content of the paper

References

1Stachler, RJ, Chandrasekhar, SS, Archer, SM, Rosenfeld, RM, Schwartz, SR, Barrs, DM et al. Clinical practice guideline: sudden hearing loss. Otolaryngol Head Neck Surg 2012;146:S1–35Google Scholar
2Hultcrantz, E, Nosrati-Zarenoe, R. Corticosteroid treatment of idiopathic sudden sensorineural hearing loss: analysis of an RCT and material drawn from the Swedish national database. Eur Arch Otorhinolaryngol 2015;272:3169–75Google Scholar
3Lim, HJ, Kim, YT, Choi, SJ, Lee, JB, Park, HY, Park, KH et al. Efficacy of 3 different steroid treatments for sudden sensorineural hearing loss: a prospective, randomized trial. Otolaryngol Head Neck Surg 2013;148:121–7Google Scholar
4Filipo, R, Attanasio, G, Russo, FY, Viccaro, M, Mancini, P, Covelli, E. Intratympanic steroid therapy in moderate sudden hearing loss: a randomized, triple-blind, placebo-controlled trial. Laryngoscope 2013;123:774–8Google Scholar
5Hou, J, She, W, Du, X, Dai, Y, Xie, L, Zhou, Q. Histone deacetylase 2 in sudden sensorineural hearing loss patients in response to intratympanic methylprednisolone perfusion. Otolaryngology Head Neck Surg 2016;154:164–70Google Scholar
6Barnes, PJ, Adcock, IM. Glucocorticoid resistance in inflammatory diseases. Lancet 2009;373:1905–17Google Scholar
7Zhou, Y, Wang, GF, Yang, L, Liu, F, Kang, JQ, Wang, RL. Treatment with 1, 25(OH)2D3 induced HDAC2 expression and reduced NF-kB p65 expression in a rat model of OVA-induced asthma. Braz J Med Biol Res 2015;48:654–64Google Scholar
8Baek, SH. When signaling kinases meet histone and histone modifiers in the nucleus. Mol Cell 2011;42:274–84Google Scholar
9Yamaqoe, S, Kanno, T, Kanno, Y, Sasaki, S, Siegel, RM, Lenardo, MJ et al. Interaction of histone acetylases and deacetylases in vivo. Mol Cell Biol 2003;23:1025–33Google Scholar
10Khan, SA, Reddy, D, Gupta, S. Global histone post-translational modifications and cancer: biomarkers for diagnosis, prognosis and treatment? World J Biol Chem 2015;6:333–45Google Scholar
11Kurdistani, SK, Tavazoie, S, Grunstein, M. Mapping global histone acetylation patterns to gene expression. Cell 2004;117:721–33Google Scholar
12She, W, Dai, Y, Du, X, Yu, C, Chen, F, Wang, J et al. Hearing evaluation of intratympanic methylprednisolone perfusion for refractory sudden sensorineural hearing loss. Otolaryngol Head Neck Surg 2010;142:266–71Google Scholar
13Yang, CH, Ko, MT, Peng, JP, Hwang, CF. Zinc in the treatment of idiopathic sudden sensorineural hearing loss. Laryngoscope 2011;121:617–21Google Scholar
14Editorial Board of Chinese Journal of Otorhinolaryngology Head and Neck Surgery; Society of Otorhinolaryngology Head and Neck Surgery, Chinese Medical Association. Guideline of diagnosis and treatment of sudden deafness (2015) [in Chinese]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2015;50:443–7Google Scholar
15Du, X, Chen, K, Kuriyavar, S, Kopke, RD, Grady, BP, Bourne, DH et al. Magnetic targeted delivery of dexamethasone acetate across the round window membrane in guinea pigs. Otol Neurotol 2013;34:41–7Google Scholar
16Shih, CP, Chen, HC, Chen, HK, Chiang, MC, Sytwu, HK, Lin, YC. Ultrasound-aided microbubbles facilitate the delivery of drugs to the inner ear via the round window membrane. J Control Release 2013;167:167–74Google Scholar
17Li, ML, Lee, LC, Cheng, YP, Kuo, CH, Chou, YF, Chen, YS et al. A novel aerosol-mediated drug delivery system for inner ear therapy: intratympanic aerosol methylprednisolone can attenuate acoustic trauma. IEEE Trans Biomed Eng 2013;60:2450–60Google Scholar
18Plontke, SK, Glien, A, Rahne, T, Mader, K, Salt, AN. Controlled release dexamethasone implants in the round window niche for salvage treatment of idiopathic sudden sensorineural hearing loss. Otol Neurotol 2014;35:1168–71Google Scholar
19Bird, PA, Begg, EJ, Zhang, M, Keast, AT, Murray, DP, Balkany, TJ. Intratympanic versus intravenous delivery of methylprednisolone to cochlear perilymph. Otol Neurotol 2007;28:1124–30Google Scholar
20Bird, PA, Murray, DP, Zhang, M, Begg, EJ. Intratympanic versus intravenous delivery of dexamethasone and dexamethasone sodium phosphate to cochlear perilymph. Otol Neurotol 2011;32:933–6Google Scholar
21Chou, YE, Chen, PR, Kuo, IJ, Yu, SH, Wen, YH, Wu, HP. Comparison of intermittent intratympanic steroid injection and near-continual transtympanic steroid perfusion as salvage treatments for sudden sensorineural hearing loss. Laryngoscope 2013;123:2264–9Google Scholar
22Erdur, O, Kayhan, FT, Cirik, AA. Effectiveness of intratympanic dexamethasone for refractory sudden sensorineural hearing loss. Eur Arch Otorhinolaryngol 2014;271:1431–6Google Scholar
23Zou, J, Poe, D, Ramadan, UA, Pyykko, I. Oval window transport of Gd-dOTA from rat middle ear to vestibulum and scala vestibule visualized by in vivo magnetic resonance imaging. Ann Otol Rhinol Laryngol 2012;121:119–28Google Scholar
24Saijo, S, Kimura, RS. Distribution of HRP in the inner ear after injection into the middle ear cavity. Acta Otolaryngol 1984;97:593–610Google Scholar
25Becker, PB, Workman, JL. Nucleosome remodeling and epigenetics. Cold Spring Harb Perspect Biol 2013;5:1–19Google Scholar
26Fu, SP, He, SY, Xu, B, Hu, CJ, Lu, SF, Shen, WX et al. Acupuncture promotes angiogenesis after myocardial ischemia through H3K9 acetylation regulation at VEGF gene. PLoS One 2014;9:e94604Google Scholar
27Kassner, SS, Schottler, S, Bonaterra, GA, Stern-Strater, J, Sommer, U, Hormann, K et al. Proinflammatory and proadhesive activation of lymphocytes and macrophages in sudden sensorineural hearing loss. Audiol Neurootol 2011;16:254–62Google Scholar
28Elliott, GO, Murphy, KJ, Hayes, JJ, Thiriet, C. Replication-independent nucleosome exchange is enhanced by local and specific acetylation of histone H4. Nucleic Acids Res 2013;41:2228–38Google Scholar
29Li, L, Zhu, J, Tian, J. Effects of histone acetylation on differentiation of mesenchymal stem cells [in Chinese]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2008;25:159–63Google Scholar
30Yin, N, Lu, R, Lin, J, Zhi, S, Tian, J, Zhu, J. Islet-1 promotes the cardiac-specific differentiation of mesenchymal stem cells through the regulation of histone acetylation. Int J Mol Med 2014;33:1075–82Google Scholar
31Kidd, SK, Schneider, JS. Protection of dopaminergic cells from MPP+- mediated toxicity by histone deacetylase inhibition. Brain Res 2010;1354:172–8Google Scholar
32Souza, NH, Marcondes, PT, Albertini, R, Mesquita-Ferrari, RA, Fernandes, KP, Aimbire, F. Low-level laser therapy suppresses the oxidative stress-induced glucocorticoids resistance in U937 cells: relevance to cytokine secretion and histone deacetylase in alveolar macrophages. J Photochem Photobiol B 2014;130:327–36Google Scholar