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Genetic polymorphisms affecting antioxidant enzymes are present in tympanosclerosis patients

Published online by Cambridge University Press:  18 August 2016

A Akyigit ,
Ş Yalcin ,
E Ö Etem ,
İ Kaygusuz ,
T Karlidag ,
E Keles ,
Z B Celik  and
A Tektemur
A Akyigit*
Affiliation:
ENT Clinic, Elazığ Education and Research Hospital
Ş Yalcin
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
E Ö Etem
Affiliation:
Department of Medical Biology, Firat University Faculty of Medicine, Elazığ, Turkey
İ Kaygusuz
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
T Karlidag
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
E Keles
Affiliation:
Department of ENT, Firat University Faculty of Medicine, Elazığ, Turkey
Z B Celik
Affiliation:
Department of Medical Biology, Firat University Faculty of Medicine, Elazığ, Turkey
A Tektemur
Affiliation:
Department of Medical Biology, Firat University Faculty of Medicine, Elazığ, Turkey
*
Address for correspondence: Dr A Akyigit, Elazığ Education and Research Hospital, 23100 Elaziğ, Turkey E-mail: [email protected]
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Abstract

Background:

This study investigated genetic polymorphisms affecting the inducible nitric oxide synthase, superoxide dismutase and catalase enzymes in chronic otitis media patients with and without tympanosclerosis, and the role of genetic susceptibility in the disease aetiology.

Methods:

A total of 162 patients who underwent surgery for chronic otitis media were divided into two study groups: a tympanosclerosis group and a chronic otitis media group. A third, the control, group comprised 188 healthy volunteers. Venous blood samples were evaluated using reverse transcriptase polymerase chain reaction.

Results:

There was a significant difference in GG genotype distribution of the −277A>G polymorphism in the NOS2 gene between the tympanosclerosis and control groups (p < 0.05). However, there were no significant between-group differences in the CC genotype distribution of the p.Ala16Val (C>T) polymorphism in the SOD2 gene (p > 0.05). There were significant differences in the TT genotype distribution of the −21A>T polymorphism in the CAT gene between the tympanosclerosis and control groups, and between the chronic otitis media and control groups (p < 0.05).

Conclusion:

These results suggest that genetic predisposition may play a role in the aetiopathogenesis of tympanosclerosis.

Keywords

Tympanosclerosis; Nitric Oxide Synthase Type II; Superoxide Dismutase; Catalase; PolymorphismGenetic
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 10 , October 2016 , pp. 928 - 933
Copyright
Copyright © JLO (1984) Limited 2016 

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References

1 Plester, D. Tympanosclerosis. J Otolaryngol Soc Aust 1972;5:325–6Google Scholar
2 Bhaya, MH, Schachern, PA, Morizono, T, Paparella, MM. Pathogenesis of tympanosclerosis. Otolaryngol Head Neck Surg 1993;109:413–20Google Scholar
3 Sheehy, JL, House, WF. Tympanosclerosis. Arch Otolaryngol 1962;76:151–7Google Scholar
4 Tos, M, Bak-Pedersen, K. Middle-ear mucosa in tympanosclerosis. J Laryngol Otol 1974;88:119–26Google Scholar
5 Beckman, JS, Beckman, TW, Chen, J. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci USA 1990;87:1620–4Google Scholar
6 Austin, DF. Reconstructive techniques for tympanosclerosis. Ann Otol Rhinol Laryngol 1988;97:670–4Google Scholar
7 Asiri, S, Hasham, A, al Anazy, F, Zakzouk, S, Banjar, A. Tympanosclerosis: review of literature and incidence among patients with middle-ear infection. J Laryngol Otol 1999;113:1076–80Google Scholar
8 Cankaya, H, Kösem, M, Kiroglu, F, Kiris, M, Yuca, K, Oztürk, G et al. Effects of topical mitomycin and trimetazidine on myringosclerosis. J Otolaryngol Head Neck Surg 2008;37:882–7Google ScholarPubMed
9 Karlıdağ, T, Ilhan, N, Kaygusuz, I, Keles, E, Yalcin, S. Comparison of free radicals and antioxidant enzymes in chronic otitis media with and without tympanosclerosis. Laryngoscope 2004;114:85–9Google Scholar
10 Görür, K, Vayisoglu, Y, Ozcan, C, Güner, N, Yaroglu, H, Tamer, L. The glutathione-S-transferase and N-acetyltranferase gene polymorphism in tympanosclerosis. Int Adv Otol 2012;8:38–44 Google Scholar
11 Lechner, M, Lirk, P, Rieder, J. Inducible nitric oxide synthase (iNOS) in tumour biology: the two sides of the same coin. Semin Cancer Biol 2005;15:277–89Google Scholar
12 Andersen, HR, Nielsen, JB, Nielsen, F, Grandjean, P. Antioxidative enzyme activities in human erythrocytes. Clin Chem 1997;43:562–8Google Scholar
13 Flekac, M, Lee, SJ, Hilgertova, J, Lacinova, Z, Jarolimkova, M. Gene polymorphisms of superoxide dismutases and catalase in diabetes mellitus. BMC Med Genet 2008;21:9–30 Google Scholar
14 Holla, LI, Stejskalova, A, Znojil, V, Vasku, A. Analysis of the inducible nitric oxide synthase gene polymorphisms in Czech patients with atopic diseases. Clin Exp Allergy 2006;36:1592–601CrossRefGoogle ScholarPubMed
15 Tos, M. Stangerup SE. Hearing loss in tympanosclerosis caused by grommets. Arch Otolaryngol Head Neck Surg 1989;115:931–5Google Scholar
16 Baysal, E, Aksoy, N, Kara, F, Taysi, S, Taskin, A, Bilinc, H et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol 2013: 270:1203–8Google Scholar
17 Yilmaz, T, Kocan, EG, Besler, HT, Yilmaz, G, Gursel, B. The role of oxidants and antioxidants in otitis media with effusion in children. Otolaryngol Head Neck Surg 2004;131:797–803 Google Scholar
18 Garcia Callejo, FJ, Estors Ferrero, J, Morant Ventura, A, Segarra Cortes, P, Velert Vila, MM. Lipoperoxidation in otorrhoea of the middle ear as a marker of infection: clinical application. Acta Otorrinolaringol Esp 2000;51:478–81Google Scholar
19 Forseni, M, Hansson, GK, Bagger-Sjöback, D, Hultcrantz, M. An immunhistochemical study of inducible nitric oxide synthase in the rat middle ear, with reference to tympanosclerosis. Acta Otolaryngol 1999;119:577–82Google Scholar
20 Atmaca, S, Erol, Y, Çoban, AY, Yildiz, L, Çengel, S, Koyuncu, M. Does exogenous nitric oxide influence tympanosclerosis? An experimental study. Int J Pediatr Otorhinolaryngol 2008;72:1845–8Google Scholar
21 Burgner, D, Xu, W, Rockett, K. Inducible nitric oxide synthase polymorphism and fatal cerebral malaria. Lancet 1998;352:1193–4Google Scholar
22 Burgner, D, Usen, S, Rockett, K. Nucleotide and haplotypic diversity of the INOS promoter region and its relationship to cerebral malaria. Hum Genet 2003;112:379–86Google Scholar
23 Forseni, M, Bagger-Sjöback, D, Hultcrantz, M. A study of inflammatory mediators in the human tympanosclerotic middle ear. Arch Otolaryngol Head Neck Surg 2001;127:559–64Google Scholar
24 Pirodda, A, Ferri, GG, Bruzzi, C, Marini, M, Faggioli, G. Possible relationship between tympanosclerosis and atherosclerosis. Acta Otolaryngol 2004;124:574–6Google Scholar
25 Quan, F, Korneluk, RG, Tropak, MB, Gravel, RA. Isolation and characterization of the human catalase gene. Nucleic Acids Res 1986;14;5321–35Google Scholar
26 Forsberg, L, de Faire, U, Morgenstern, R. Oxidative stress, human genetic variation, and disease. Arch Biochem Biophys 2001;389:84–93 Google Scholar
27 Brose, RD, Avramopoulos, D, Smith, KD. SOD2 as a potential modifier of X-linked adrenoleukodystrophy clinical phenotypes. J Neurol 2012;259:1440–7Google Scholar