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Dual silencing of epidermal growth factor and insulin-like growth factor 1 receptors significantly limits growth of nasopharyngeal carcinoma in nude mice

Published online by Cambridge University Press:  01 May 2008

X Zhou ,
Y Yuan ,
J Song ,
W Chen ,
J Li ,
L Ye ,
X Meng  and
D Xia
X Zhou
Affiliation:
Department of Otolaryngology-Head & Neck Surgery, Zhongnan Hospital of Wuhan University, China
Y Yuan*
Affiliation:
Department of Anatomy, Wuhan University School of Medicine, China
J Song
Affiliation:
Department of Anatomy, Wuhan University School of Medicine, China Key Laboratory of Allergy and Immune-related Diseases and the Center for Medical Research, Wuhan University, China
W Chen
Affiliation:
Experimental Animal Centre, Zhongnan Hospital of Wuhan University, China
J Li
Affiliation:
Department of Otolaryngology-Head & Neck Surgery, Zhongnan Hospital of Wuhan University, China
L Ye
Affiliation:
Department of Otolaryngology-Head & Neck Surgery, Zhongnan Hospital of Wuhan University, China
X Meng
Affiliation:
Department of Forensic Medicine, Wuhan University School of Medicine, China
D Xia
Affiliation:
Department of Pathology, Wuhan University School of Medicine, China
*
Address for correspondence: Prof Yulin Yuan, Department of Anatomy, Wuhan University School of Medicine, 135 Donghu Road, Wuhan, Hubei 430071 PRChina. Fax:  +86 27 87307966 E-mail: [email protected]
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Abstract

Objective:

We examined the effects of dual silencing of epidermal growth factor and insulin-like growth factor 1 receptors on the growth of nasopharyngeal carcinoma in nude mice; we also assessed potential side effects in these animals.

Methods:

Short hairpin ribonucleic acid expression vectors targeting epidermal growth factor and insulin-like growth factor 1 receptors were constructed. Short hairpin ribonucleic acid plasmids targeting one or both receptors were transfected into human nasopharyngeal carcinoma cells in nude mice. We then assessed epidermal growth factor receptor and insulin-like growth factor 1 receptor expression and also cellular apoptosis. Peripheral blood was collected and subjected to haematological and biochemical analysis.

Results:

The findings demonstrated that transfection with dual plasmids (targeting both epidermal growth factor receptor and insulin-like growth factor 1 receptor) resulted in tumour cell growth inhibition of 84.78 per cent, and a significant increase in the number of necrotic and apoptotic cells, compared with single plasmid treatment. The short hairpin ribonucleic acid had no significant side effects on the heart, liver, kidney, spleen or blood system in this experimental model.

Conclusion:

These results indicate that, in nude mice, dual silencing of both epidermal growth factor and insulin-like growth factor 1 receptors results in more apoptosis and greater nasopharyngeal cancer cell growth inhibition, compared with silencing of either epidermal growth factor receptor alone or insulin-like growth factor 1 receptor alone. This occurred without significant side effects in the experimental animals.

Keywords

Small Hairpin RNAEGF ReceptorIGF-1 ReceptorNasopharynx NeoplasmsCarcinoma
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 123 , Issue 2 , February 2009 , pp. 208 - 222
Copyright
Copyright © JLO (1984) Limited 2008

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