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DNA indices of primary and recurrent squamous cell carcinomas of the tongue and tonsil using image cytometry

Published online by Cambridge University Press:  29 June 2007

S. K. Sarker ,
P. Tierney ,
K. S. Patel ,
C. Fisher  and
D. V. Coleman
S. K. Sarker*
Affiliation:
Cytopathology Unit, St Mary's Hospital Medical School, Imperial College Of Science, Technology and Medicine, University of London, London W2 1NY Academic Surgical Unit, St Mary's Hospital Medical School, Imperial College Of Science, Technology and Medicine, University of London, London W2 1NY Head and Neck Unit, St Mary's Hospital Medical School, Imperial College Of Science, Technology and Medicine, University of London, London W2 1NY
P. Tierney
Affiliation:
The Head and Neck Unit, Royal Marsden Hospital, London SW3 6JJ, UK
K. S. Patel
Affiliation:
Head and Neck Unit, St Mary's Hospital Medical School, Imperial College Of Science, Technology and Medicine, University of London, London W2 1NY The Head and Neck Unit, Royal Marsden Hospital, London SW3 6JJ, UK
C. Fisher
Affiliation:
Histopathology Unit, Royal Marsden Hospital, London SW3 6JJ, UK
D. V. Coleman
Affiliation:
Cytopathology Unit, St Mary's Hospital Medical School, Imperial College Of Science, Technology and Medicine, University of London, London W2 1NY
*
Address for correspondence: S. K. Sarker, Cytopathology and Cytogenetics Unit, Clarence Wing, St Mary's Hospital Medical School, Praed Street, London W2 1NY.
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Abstract

Ploidy status of squamous cell carcinomas of the head and neck (SCCHN) from primary and recurrent tonsillar and tongue lesions has not been compared using image cytometry. We have measured and compared the DNA indices in 41 cases. There were 29 tongue SCCHN, 20/29 were primary and 9/29 were recurrent. Mean DNA index (DI) was 1.19 (range 0.70–1.81) and 1.28 (range 0.79–1.94) respectively. There were 12 tonsillar cases, 10/12 primary and two out of 12 recurrent. Mean DI was 0.84 (range 0.57–1.09) and 1.00 (range 0.98–1.02) respectively. Mean DNA indices of both primary carcinomas were lower than the mean DNA indices of the recurrent carcinomas. This difference between the two groups may be a reflection of their tumour biology. However, since our study is small no definite conclusions can be made at this stage. We aim in the future to evaluate the prognostic role of DNA indices of patients with paired primary and recurrent SCCHN. This may be of clinical value and improve the treatment modalities available to this group.

Keywords

Image cytometryPloidiesHead and neck neoplasmsTongueTonsilCarcinoma, squamous cell
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 110 , Issue 3 , March 1996 , pp. 237 - 239
Copyright
Copyright © JLO (1984) Limited 1996

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References

Bacus, J. W., Grace, L. J. (1987) Optical microscope system for standardized cell measurements and analysis. Applied Optics 26: 3280–3293.Google Scholar
Boecking, A., Auffermann, W., Vogel, H., Schloloendorff, G., Goebbels, R. (1985) Diagnosis and grading of malignancy in squamous epithelial lesions of the larynx with DNA cytophotometry. Cancer 56: 1600–1604.Google Scholar
Deitch, A. (1966) Cytophotometry of nucleic acids. In Introduction to quantitative cytochemistry. (Weid, G. L., ed.) Academic Press, London, pp 327–354.Google Scholar
Ensley, J. F., Maciorowski, Z., Hassan, M., Pietraszkiewicz, H., Heilburn, L., Kish, J. A., Tapazoglou, E., Jacobs, J. R., Al-Sarraf, M. (1989) Cellular DNA content parameters in untreated and recurrent squamous cell cancers of the head and neck. Cytometry 10: 334–338.Google Scholar
Fu, Y. S., Hall, T. L. (1985) DNA ploidy measurements in tissue sections. Analytical Quantitative Cytology and Histology 7: 90.Google ScholarPubMed
Goldsmith, M. M., Cresson, D. H., Arnold, L. A., Postma, D. S., Askin, F. B., Pillsbury, H. C. (1987) DNA flow cytometry as a prognostic indicator in head and neck cancer. Otolaryngology-Head and Neck Surgery 96: 307–318.CrossRefGoogle ScholarPubMed
Guo, Y. C, Desanto, L., Oestinsky, G. V. (1989) Prognostic implications of nuclear DNA content in head and neck cancer. Otolaryngology-Head and Neck Surgery 100: 95–98.CrossRefGoogle ScholarPubMed
Holm, L. (1982) Cellular DNA amounts of squamous cell carcinomas of the head and neck region in relation to prognosis. Laryngoscope 92: 1064–1069.Google Scholar
Kearsley, J. H., Bryson, G., Battistutta, D., Collins, R. J. (1991) Prognostic importance of cellular DNA content in head and neck squamous cell cancers. A comparison of retrospective and prospective series. International Journal of Cancer 47: 31–37.Google Scholar
Kokal, W. A., Gardiner, R. L., Sheibani, K., Zak, I., Beatty, D., Riihimaki, D. U., Wagman, L. D., Terz, J. J. (1988) Tumour DNA content as a prognostic indicator in squamous cell carcinoma of the head and neck region. American Journal of Surgery 156: 276–280.Google Scholar
Mayall, B. H. (1988) Current capabilities and clinical applications of image cytometry. Cytometry 3 (suppl): 78–84.CrossRefGoogle ScholarPubMed
Raslan, W. F., Barnes, L., Krause, J. R., Contis, L., Killeen, R., Kapachia, S. B. (1994) Baseloid squamous cell carcinoma of the head and neck: a clinopathologic and flow cytometric study of 10 new cases with a review of the English literature. American Journal of Otolaryngology 15(3): 204–211.Google Scholar
Sandwritter, W., Carl, M, Ritter, W. (1966) Cytomorphometric measurements of the DNA content of human malignant tumors by means of the Feulgen reaction. Acta Cytologica (Baltimore) 10: 26–30.Google Scholar
Sarker, S. K., Coleman, D. V. (1995b) Measurement of DNA indices using image cytometry of breast cancer. Analytical Quantitative Cytology and Histology (submitted).Google Scholar
Sarker, S. K., Patel, K. S., Tolley, N. S., Coleman, D. V. (1996) DNA ploidy using image cytometry of squamous cell carcinomas of the head and neck. Journal of Laryngology and Otology (in press).CrossRefGoogle Scholar
Westerbeek, H. A., Mooi, W. J., Hilgers, F. J. M., Baris, G., Begg, A. C, Begg, A. C, Balm, A. J. M. (1993) Ploidy status and the response of Tl glottic carcinoma to radiotherapy. Clinical Otolaryngology 18: 98–101.Google Scholar