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Comparison of the proteins of middle ear effusion with human mast cell proteins

Published online by Cambridge University Press:  29 June 2007

Yoseph Rakover ,
Amir Shneyour ,
Gabriel Rosen  and
Yaacov Lensky
Yoseph Rakover
Affiliation:
Department of Otorhinolaryngology, Central Emek Hospital, Afula, Israel
Amir Shneyour
Affiliation:
Department of the Clinical Immunology Laboratory, Central Emek Hospital, Afula, Israel
Gabriel Rosen
Affiliation:
Department of Otorhinolaryngology, Central Emek Hospital, Afula, Israel
Yaacov Lensky*
Affiliation:
Hebrew University, Faculty of Agriculture, Israel.
*
Y. Rakover, Department of Otorhinolaryngology, Central Emek Hospital, Afula, Israel.
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Abstract

In order to clarify the role of mast cells in the aetiology of secretory otitis media (SOM), we compared the protein components of middle ear effusion (MEE) with human mast cells using acrylamide gradient gel electrophoresis and electrofocusing methods. This first direct comparison between the proteins of MEE and human mast cells has been made possible by a method developed in our laboratory for cultivation of human mast cells in tissue culture.

On electrophoresis, we found that out of 12 bands of MEE proteins that were different from the serum, seven (58 per cent) had a similar electrophoretic migration rate (Rx) to mast cells. On electrofocusing, three of the four bands of MEE had a similar Rx to the mast cells. We have shown that proteins of mast cells and MEE had similar Rxs. Therefore, our study supports previous studies which suggests that mast cells play an important role in the aetiology of SOM.

Keywords

Otitis media with effusionMast cells
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 109 , Issue 12 , December 1995 , pp. 1146 - 1150
Copyright
Copyright © JLO (1984) Limited 1995

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