Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-05T19:53:35.629Z Has data issue: false hasContentIssue false
  • English
  • Français

Cilia from a cystic fibrosis patient react to the ciliotoxic Pseudomonas aeruginosa II lectin in a similar manner to normal control cilia – a case report

Published online by Cambridge University Press:  29 June 2007

Elizabeth C. Adam ,
Dietlind U. Schumacher  and
Udo Schumacher
Elizabeth C. Adam*
Affiliation:
Department of Human Morphology, University of Southampton, Royal South Hants Hospital, Southampton, UK.
Dietlind U. Schumacher
Affiliation:
ENT Department, Royal South Hants Hospital, Southampton, UK.
Udo Schumacher
Affiliation:
Department of Human Morphology, University of Southampton, Royal South Hants Hospital, Southampton, UK.
*
Address for correspondence: E. C. Adam, Human Morphology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX. Fax: +44 (0)1703 594433 email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The ciliary beat frequency measurements taken from a nasal polyp from a cystic fibrosis patient were similar to that of the control nasal polyps. The addition of a ciliotoxic lectin produced by Pseudomonas aeruginosa stopped the beating of the cilia as in the controls. This reaction could be blocked by the pre-incubation of the lectin with its inhibitor fucose. As in the control, the addition of fucose after the cilia had slowed resulted in a return to normal ciliary beating within 24 hours. This shows that the delta F508 CF mutation observed in this patient does not affect ciliary beating and suggests that treatment with fucose in the early stages of a Pseudomonas aeruginosa infection could be advantageous for cystic fibrosis patients.

Keywords

CiliaPseudomonas aeruginosaLectins, Cystic fibrosis
Type
Clinical Records
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 8 , August 1997 , pp. 760 - 762
Copyright
Copyright © JLO (1984) Limited 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adam, E. C., Mitchell, B. S., Schumacher, D. U., Grant, G., Schumacher, U. (1997) Pseudomonas aeruginosa II lectin stops human ciliary beating: therapeutic implications of fucose. American Journal of Respiratory and Critical Care Medicine 155: 2102–2104.Google Scholar
De Bentzmann, S., Roger, P., Puchelle, E. (1996) Pseudomonas aeruginosa adherence to remodelling respiratory epithelium. European Respiratory Journal 9: 2145–2150.Google Scholar
Fick, R. B., Baltimore, R. S., Squier, S. V., Reynolds, S. Y. (1985) IgG proteolytic activity of Pseudomonas aeruginosa in cystic fibrosis. Journal of Infectious Diseases 151: 589–598.CrossRefGoogle ScholarPubMed
Hingley, S. T., Hastie, A. T., Kueppers, F., Higgins, M. L., Weinbaum, G., Shryock, T. (1986) Effect of ciliostatic factors from Pseudomonas aeruginosa on rabbit respiratory cilia. Infection and Immunity 51: 254–262.Google Scholar
Ko, Y. H., Delannoy, M., Pederson, P. L. (1997) Cystic fibrosis, lung infections, and a human tracheal antimicrobial peptide (hTAP). FEBS Letters 405: 200–208.CrossRefGoogle Scholar
Warner, J. O. (1992) Immunology of cystic fibrosis. British Medical Bulletin 48: 893–911.CrossRefGoogle ScholarPubMed