Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-17T16:50:14.643Z Has data issue: false hasContentIssue false
  • English
  • Français

Autologous fat injection therapy including a high concentration of adipose-derived regenerative cells in a vocal fold paralysis model: animal pilot study

Published online by Cambridge University Press:  08 September 2016

N Nishio ,
Y Fujimoto ,
K Suga ,
Y Iwata ,
K Toriyama ,
K Takanari ,
Y Kamei ,
T Yamamoto  and
M Gotoh
N Nishio*
Affiliation:
Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, Aichi, Japan
Y Fujimoto
Affiliation:
Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, Aichi, Japan
K Suga
Affiliation:
Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, Aichi, Japan
Y Iwata
Affiliation:
Department of Otorhinolaryngology, Fujita Health University, Aichi, Japan
K Toriyama
Affiliation:
Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan
K Takanari
Affiliation:
Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan
Y Kamei
Affiliation:
Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan
T Yamamoto
Affiliation:
Department of Urology, Nagoya University Graduate School of Medicine, Aichi, Japan
M Gotoh
Affiliation:
Department of Urology, Nagoya University Graduate School of Medicine, Aichi, Japan
*
Address for correspondence: Dr Naoki Nishio, Department of Otorhinolaryngology, Nagoya University Graduate School of Medicine, 65, Tsurumai-cho, Showa-ku, Nagoya 466–8550, Japan Fax: +11 81 52 744 2325 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objectives:

To verify the effectiveness and safety of the addition of adipose-derived regenerative cells to autologous fat injection therapy.

Methods:

Unilateral vocal fold paralysis models were made by cutting the right recurrent laryngeal nerve in two pigs. At day 30, 0.5 ml adipose-derived regenerative cells mixed with 1 ml autologous fat was injected into the right vocal fold of one pig, with the other receiving 0.5 ml Ringer's solution mixed with 1 ml autologous fat. At day 120, fibrescopy, laser Doppler flowmeter, computed tomography, vocal function evaluation and histological assessment were conducted.

Results:

Although histological assessment revealed atrophy of the thyroarytenoid muscle fibre in both pigs, there was remarkable hypertrophy of the thyroarytenoid muscle fibre in the area surrounding the adipose-derived regenerative cells injection site.

Conclusion:

The addition of a high concentration of adipose-derived regenerative cells to autologous fat injection therapy has the potential to improve the treatment outcome for unilateral vocal fold paralysis.

Keywords

Stem CellsInjectionsVocal Cord ParalysisAnimalHoarseness
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 10 , October 2016 , pp. 914 - 922
Copyright
Copyright © JLO (1984) Limited 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Mikaelian, DO, Lowry, LD, Sataloff, RT. Lipoinjection for unilateral vocal cord paralysis. Laryngoscope 1991;101:465–8CrossRefGoogle ScholarPubMed
2 McCulloch, TM, Andrews, BT, Hoffman, HT, Graham, SM, Karnell, MP, Minnick, C. Long-term follow-up of fat injection laryngoplasty for unilateral vocal cord paralysis. Laryngoscope 2002;112:1235–8CrossRefGoogle ScholarPubMed
3 Tamura, E, Fukuda, H, Tabata, Y. Adipose tissue formation in response to basic fibroblast growth factor. Acta Otolaryngol 2007;127:1327–31CrossRefGoogle ScholarPubMed
4 Umeno, H, Chitose, S, Murofushi, Y, Kosai, K, Sato, K, Kawahara, A et al. Efficacy of autologous fat injection laryngoplasty with an adenoviral vector expressing hepatocyte growth factor in a canine model. J Laryngol Otol 2009;123:24–9CrossRefGoogle Scholar
5 Pittenger, MF, Mackay, AM, Beck, SC, Jaiswal, RK, Douglas, R, Mosca, JD et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143–7CrossRefGoogle ScholarPubMed
6 Umezawa, A, Maruyama, T, Segawa, K, Shadduck, RK, Waheed, A, Hata, J. Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo. J Cell Physiol 1992;151:197–205 CrossRefGoogle ScholarPubMed
7 Zuk, PA, Zhu, M, Mizuno, H, Huang, J, Futrell, JW, Katz, AJ et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 2001;7:211–28CrossRefGoogle ScholarPubMed
8 Fraser, JK, Wulur, I, Alfonso, Z, Hedrick, MH. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 2006;24:150–4CrossRefGoogle ScholarPubMed
9 Nagaya, N, Kangawa, K, Itoh, T, Iwase, T, Murakami, S, Miyahara, Y et al. Transplantation of mesenchymal stem cells improves cardiac function in a rat model of dilated cardiomyopathy. Circulation 2005;112:1128–35CrossRefGoogle Scholar
10 Safford, KM, Hicok, KC, Safford, SD, Halvorsen, YD, Wilkison, WO, Gimble, JM et al. Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem Biophys Res Commun 2002;294:371–9CrossRefGoogle ScholarPubMed
11 Gotoh, M, Yamamoto, T, Kato, M, Majima, T, Toriyama, K, Kamei, Y et al. Regenerative treatment of male stress urinary incontinence by periurethral injection of autologous adipose-derived regenerative cells: 1-year outcomes in 11 patients. Int J Urol 2014;21:294–300 CrossRefGoogle ScholarPubMed
12 Yamamoto, T, Gotoh, M, Hattori, R, Toriyama, K, Kamei, Y, Iwaguro, H et al. Periurethral injection of autologous adipose-derived stem cells for the treatment of stress urinary incontinence in patients undergoing radical prostatectomy: report of two initial cases. Int J Urol 2010;17:75–82 CrossRefGoogle ScholarPubMed
13 Yamamoto, T, Gotoh, M, Kato, M, Majima, T, Toriyama, K, Kamei, Y et al. Periurethral injection of autologous adipose-derived regenerative cells for the treatment of male stress urinary incontinence: report of three initial cases. Int J Urol 2012;19:652–9CrossRefGoogle ScholarPubMed
14 Lo Cicero, V, Montelatici, E, Cantarella, G, Mazzola, R, Sambataro, G, Rebulla, P et al. Do mesenchymal stem cells play a role in vocal fold fat graft survival? Cell Prolif 2008;41:460–73CrossRefGoogle ScholarPubMed
15 Kruschewsky Lde, S, de Mello-Filho, FV, dos Santos, AC, Rosen, CA. Autologous fat graft absorption in unilateral paralyzed canine vocal folds. Laryngoscope 2007;117:96–100 CrossRefGoogle ScholarPubMed
16 Lin, K, Matsubara, Y, Masuda, Y, Togashi, K, Ohno, T, Tamura, T et al. Characterization of adipose tissue-derived cells isolated with the Celution system. Cytotherapy 2008;10:417–26CrossRefGoogle ScholarPubMed
17Praat: doing phonetics by computer. In: www.praat.org [21 July 2016]Google Scholar
18 Kanemaru, S, Nakamura, T, Omori, K, Kojima, H, Magrufov, A, Hiratsuka, Y et al. Regeneration of the vocal fold using autologous mesenchymal stem cells. Ann Otol Rhinol Laryngol 2003;112:915–20CrossRefGoogle ScholarPubMed
19 Hertegård, S, Cedervall, J, Svensson, B, Forsberg, K, Maurer, FH, Vidovska, D et al. Viscoelastic and histologic properties in scarred rabbit vocal folds after mesenchymal stem cell injection. Laryngoscope 2006;116:1248–54CrossRefGoogle ScholarPubMed
20 Ohno, S, Hirano, S, Kanemaru, S, Mizuta, M, Ishikawa, S, Tateya, I et al. Role of circulating MSCs in vocal fold wound healing. Laryngoscope 2012;122:2503–10CrossRefGoogle ScholarPubMed
21 Kanemaru, S, Nakamura, T, Yamashita, M, Magrufov, A, Kita, T, Tamaki, H et al. Destiny of autologous bone marrow-derived stromal cells implanted in the vocal fold. Ann Otol Rhinol Laryngol 2005;114:907–12CrossRefGoogle ScholarPubMed
22 Lee, BJ, Wang, SG, Lee, JC, Jung, JS, Bae, YC, Jeong, HJ et al. The prevention of vocal fold scarring using autologous adipose tissue-derived stromal cells. Cells Tissues Organs 2006;184:198–204 CrossRefGoogle ScholarPubMed
23 Chhetri, DK, Head, C, Revazova, E, Hart, S, Bhuta, S, Berke, GS. Lamina propria replacement therapy with cultured autologous fibroblasts for vocal fold scars. Otolaryngol Head Neck Surg 2004;131:864–70CrossRefGoogle ScholarPubMed
24 Cedervall, J, Ahrlund-Richter, L, Svensson, B, Forsgren, K, Maurer, FH, Vidovska, D et al. Injection of embryonic stem cells into scarred rabbit vocal folds enhances healing and improves viscoelasticity: short-term results. Laryngoscope 2007;117:2075–81CrossRefGoogle ScholarPubMed
25 Goding, GS, Richardson, MA, Trachy, RE. Laryngeal chemoreflex: anatomic and physiologic study by use of the superior laryngeal nerve in the piglet. Otolaryngol Head Neck Surg 1987;97:28–38 CrossRefGoogle ScholarPubMed
26 Woodson, G. Developing a porcine model for study of vocal fold scar. J Voice 2012;26:706–10CrossRefGoogle ScholarPubMed
27 Jiang, JJ, Raviv, JR, Hanson, DG. Comparison of the phonation-related structures among pig, dog, white-tailed deer, and human larynges. Ann Otol Rhinol Laryngol 2001;110:1120–5CrossRefGoogle ScholarPubMed
28 Garrett, CG, Coleman, JR, Reinisch, L. Comparative histology and vibration of the vocal folds: implications for experimental studies in microlaryngeal surgery. Laryngoscope 2000;110:814–24CrossRefGoogle ScholarPubMed
29 Hahn, MS, Kobler, JB, Starcher, BC, Zeitels, SM, Langer, R. Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid. Ann Otol Rhinol Laryngol 2006;115:156–64CrossRefGoogle ScholarPubMed
30 Alipour, F, Jaiswal, S. Phonatory characteristics of excised pig, sheep, and cow larynges. J Acoust Soc Am 2008;123:4572–81CrossRefGoogle ScholarPubMed
31 Alipour, F, Jaiswal, S, Vigmostad, S. Vocal fold elasticity in the pig, sheep, and cow larynges. J Voice 2011;25:130–6CrossRefGoogle ScholarPubMed