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Are laryngeal papilloma virus-infected cells viable in the plume derived from a continuous mode carbon dioxide laser, and are they infectious? A preliminary report on one laser mode

Published online by Cambridge University Press:  29 June 2007

Somyos Kunachak ,
Pranee Slthisarn  and
Boonchu Kulapaditharom
Somyos Kunachak*
Affiliation:
Department of Otolaryngology, Faculty of Science, Mahidol University School of Medicine, Bangkok 10400, Thailand
Pranee Slthisarn
Affiliation:
Ramathibodi Hospital and the Department of Microbiology, Faculty of Science, Mahidol University School of Medicine, Bangkok 10400, Thailand
Boonchu Kulapaditharom
Affiliation:
Department of Otolaryngology, Faculty of Science, Mahidol University School of Medicine, Bangkok 10400, Thailand
*
Address for correspondence: Somyos Kunachak, M.D., Department of Otolaryngology, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok 10400, Thailand. Fax: 662-2538901
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Abstract

The purpose of this study was to determine the potential risk of transmitting viable viral infected cells as well as viral infectivity of laryngeal papilloma in the plume derived from a continuous mode carbon dioxide laser. Each of 10 juvenile recurrent laryngeal papilloma specimens was divided into two equal parts, and one part was irradiated with a carbon dioxide laser employing a continuous mode at the power setting of 10 watts with 0.5 mm spot size and a power density of 1667 W/cm2. The resultant laser plume was trapped and was cultured simultaneously with the other part of the specimen which served as the control. All irradiated specimens tested yielded negative culture results while all the control counterparts revealed viable cell growth. To detect the viral infectivity, laser plume was cultured with two separate cellsystems, one was the porcine PS clone D cell line and the other normal mucosal cells obtained from the same patient, and to control these systems both cell lines were also designed to be infected with polio virus. Both cell lines in the viral infectivity testing systems revealed no sign of viral infection. The results suggest that papilloma virus-infected cells cannot survive the continuous mode of carbon dioxide laser irradiation. We primarily conclude that, to avoid airborne transmission of plume containing laryngeal papilloma viral-infected cells and infectious viral particles, the carbon dioxide laser parameters should be in a continuous mode with the power density equal to, or more than, 1667 W/cm2.

Keywords

Laryngeal neoplasmsPapillomaLaser surgery
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 110 , Issue 11 , November 1996 , pp. 1031 - 1033
Copyright
Copyright © JLO (1984) Limited 1996

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References

Abramson, A. L., Dilorenzo, T. P., Steinberg, B. M. (1990) Papillomavirus disturbance in the plume of laser-treated laryngeal papilloma? Archives of Otolaryngology, Head and Neck Surgery 16: 604–607.CrossRefGoogle Scholar
Ediger, M. N., Matchette, L. S. (1989) In vitro production of viable bacteriophage in a laser plume. Lasers in Surgery and Medicine 9: 296–299.CrossRefGoogle Scholar
Freitag, L., Chapman, G. A., Sielczak, M., Ahmed, A., Russin, D. (1987) Laser smoke effect on the bronchial system. Lasers in Surgery and Medicine 3: 283–288.CrossRefGoogle Scholar
Kashima, H. K., Kessis, T., Mounts, P., Shah, K. (1991) Polymerase chain reaction identification of human papillomavirus DNA in CO2 laser plume from recurrent respiratory papillomatosis. Otolaryngology-Head and Neck Surgery 104: 191–195.CrossRefGoogle ScholarPubMed
Matchette, L. S., Vegella, T. J., Faaland, R. W. (1993) Viable bacteriophage in CO2 laser plume. Lasers in Surgery and Medicine 13: 18–22.CrossRefGoogle ScholarPubMed
Mihashi, S., Jako, G. J., Incze, J., Strong, M. S., Vanghn, C. W. (1975) Laser surgery in Otolaryngology. Interaction of CO2 laser and soft tissue. Annals of the New York Academy of Science 267: 263–294.CrossRefGoogle Scholar
Mounts, P., Shah, K. (1984) Respiratory papillomatosis: etiological relation to genital tract papillomaviruses. Progress in Medical Virology 29: 90–114.Google ScholarPubMed
Mullarky, M. B., Norris, C. W., Goldberg, I. D. (1985) The efficacy of the CO2 laser in the sterilization of skin surface and in the plume emissions. Laryngoscope 95: 186–187.CrossRefGoogle ScholarPubMed
Nezhat, C., Winer, W. K., Nezhat, F., Nezhat, C., Farrest, D., Reeves, W. G. (1987) Smoke from laser surgery: Is there a health hazard? Lasers in Surgery and Medicine 7: 376–382.CrossRefGoogle Scholar
Oosterhius, J. W., Verschveren, R. C. J., Eibergen, R., Oldhoff, J. (1982) The viability of cells in the waste products of CO2 laser evaporation of cloudman mouse melanomas. Cancer 49: 61–67.3.0.CO;2-N>CrossRefGoogle Scholar
Sawchuk, W. S., Weber, P. J., Lowy, D. R., Dzubow, L. M. (1989) Infectious papillomavirus in the vapour of warts treated with carbon dioxide laser or electrocoagulation: Detection and protection. Journal of the American Academy of Dermatology 21: 41–49.CrossRefGoogle ScholarPubMed
Schmidt, N. J. (1989) Cell culture procedures for diagnostic virology. In Diagnostic procedures for viral, rickettsial and chamydial infections. 6th Edition (Schmidt, N. J., Emmons, R. W., eds.) American Public Health Association Inc., Washington, pp 51–100.Google Scholar
Starr, J. C., Kilmer, S., Wheeland, R. G. (1992) Analysis of the carbon dioxide laser plume for Simian Immunodeficiency Virus. Journal of Dermatologic Surgery and Oncology 8: 297–300.CrossRefGoogle Scholar
Voorhies, R. M., Lavyne, M. H., Strait, T. A., Shapiro, W. R. (1984) Does the CO2 laser spread viable brain-tumor cells outside the surgical field? Journal of Neurosurgery 60: 819–820.CrossRefGoogle ScholarPubMed
Walker, N. P. J., Matthews, J., Newsom, S. W. B. (1986) Possible hazards from irradiation with the carbon dioxide lasers. Lasers in Surgery and Medicine 6: 84–86.CrossRefGoogle Scholar
Weisberger, E. C. (1991) Laryngology. In Lasers in Head and Neck Surgery. 1st Edition. (Weisberger, E. C., ed.), Igaku-Shoin, New York, pp 197–220.Google Scholar