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Absence of Helicobacter pylori in healthy laryngeal mucosa

Published online by Cambridge University Press:  01 November 2011

I Pajić-Penavić ,
D Đanić ,
S Maslovara  and
K Gall-Trošelj
I Pajić-Penavić*
Affiliation:
ENT Department, Dr Josip Benčević General Hospital, Slavonski Brod, Croatia
D Đanić
Affiliation:
ENT Department, Dr Josip Benčević General Hospital, Slavonski Brod, Croatia
S Maslovara
Affiliation:
ENT Department, Vukovar General Hospital, Zagreb, Croatia
K Gall-Trošelj
Affiliation:
Laboratory of Epigenomics, Ruđer Bošković Institute, Zagreb, Croatia
*
Address for correspondence: Dr Ivana Pajić-Penavić, Kneza Domagoja 4, 35 000 Slavonski Brod, Croatia Fax: +385 35 446 177 E-mail: [email protected]
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Abstract

Objectives:

To evaluate the presence of Helicobacter pylori in healthy laryngeal mucosa.

Design:

Prospective analysis of Helicobacter pylori colonisation in healthy laryngeal mucosa, using the 13C urea breath test and polymerase chain reaction analysis.

Subjects:

Twenty randomly chosen men (28–78 years) without laryngeal pathology or gastrointestinal disease were investigated. All subjects were scheduled for elective operative procedures, under general, endotracheal anaesthesia. Cytobrush samples were taken for Helicobacter pylori DNA detection. Nested polymerase chain reaction testing was performed on DNA solutions using two primer pairs from the urease A gene of the Helicobacter pylori genome. The 13C urea breath test was performed on two exhalation samples.

Results:

Eight (40 per cent) of the subjects were positive for urease on urea breath testing; none were positive for Helicobacter pylori DNA on polymerase chain reaction testing.

Conclusion:

Based on these results, we do not consider Helicobacter pylori to be a normal constituent of healthy laryngeal microflora.

Keywords

Helicobacter PyloriMicrobiologyBacteriologyLarynxPolymerase Chain Reaction
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 2 , February 2012 , pp. 196 - 199
Copyright
Copyright © JLO (1984) Limited 2011

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