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Variations in the Metabolism of the Daughter Sporocysts of Microphallus pygmaeus in a Chemically Defined Medium

Published online by Cambridge University Press:  05 June 2009

R. J. Richards ,
D. Pascoe  and
B. L. James
R. J. Richards
Affiliation:
Department of Zoology, University College of Swansea, Wales, U.K.
D. Pascoe
Affiliation:
Department of Zoology, University College of Swansea, Wales, U.K.
B. L. James
Affiliation:
Department of Zoology, University College of Swansea, Wales, U.K.
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Extract

A comparison is made of the variations in the mean size (length), reduced weight, oxygen uptake, metabolic rate and the number of contained fully formed metacercariae undergoing autolysis, in mature daughter sporocysts of Microphallus pygmaeus, in sea water, artificial sea water and in a chemically defined nutrient medium (modified medium 199). The work indicates that the sporocysts begin to degenerate almost immediately in the non-nutrient media but have a higher metabolic activity and remain healthy for up to 36 days in the nutrient medium.

Type
Research Article
Information
Journal of Helminthology , Volume 46 , Issue 1 , March 1972 , pp. 107 - 116
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Barnes, , 1905.—Biological Laboratory Data 2nd. Ed. (Hale, L. J. ed.) Methuen & Co. Ltd. London.Google Scholar
Von Brand, T., 1966.—The Biochemistry of Parasites. Academic Press N.Y. and Lond. 429 pp.Google Scholar
Imohiosen, E., 1969.—“The biology of larval Digenea from littoral molluscs: The ultrastructure and enzyme histochemistry of germinal sacs.” Ph.D. Thesis. University College, Swansea.Google Scholar
James, B. L. 1905.—“The effects of parasitism by larval Digenea on the digestive gland of the intertidal prosobranch, Littorina saxatilis (Olivi) subsp. tenebrosa (Montagu).” Parasitology, 55, 93–115.Google Scholar
James, B. L. and Bowers, E. A., 1907a.—“Histochemical observations on the occurrence of carbohydrates, lipids and enzymes in the daughter sporocysts of Cercaria bucephalopsis hiamena (Lacaze-Duthiers, 1854). (Digenea: Buccphalidae).” Parasitology, 57, 79–80.Google Scholar
James, B. L. and Bowers, E. A., 1967a.—“The effects of parasitism by the daughter sporocysts of Cercaria bucephalopsis hiamena (Lacaze-Duthiers, 1854) on the digestive tubules of the cockle, Cardittm edule L.” Parasitology, 57, 67–77.CrossRefGoogle Scholar
Jensen, D. V. and Stirewalt, M. A., 1963.—“Development of cercariae of Schistosoma mansoni under dialysis membranes in Rose multipurpose chambers.” J. Parasit., 49, 61.Google Scholar
Jensen, D. V., AND Walters, M., 1905.—“Growth of Schistosoma mansoni cercariae under dialysis membranes in Rose multipurpose chambers.” Expl. Parasit., 17, 15–23.Google Scholar
McDaniel, J. S. and Dixon, K. E., 1907.—“Utilisation of exogenous glucose by the rcdiae of Parorchis acanthus (Digenea: Philophthalmidae) and Cryptocotyle lingua (Digenea: Heterophyidae).” Biol. bull. mar. biol. Lab. Woods Hole 133, 591–599.Google Scholar
Morgan, J. G., Morton, H. J. and Parker, R. C., 1950.—“Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc. Soc. exp. Biol. Med., 73, 1–8.Google Scholar
Muftic, M., 1909.—“Metamorphosis of miracidia into cercariae of Schistosoma mansoni in vitro.” Parasitology, 59, 305–371.Google Scholar
Pascoe, D., 1909.—“The oxygen uptake, reduced weight and number of con- tained cercariae in the germinal sacs of 5 species of Digenea from inter- tidal molluscs.” Ph.D. Thesis, University College, Swansea.Google Scholar
Pascoe, D., 1970.—“Dehydrogenases in the daughter sporocysts of Microphallus pygmaeus (Levinsen, 1881) (Trcmatoda: Microphallidae).” Z. ParasitenKde, 35, 7–15.Google Scholar
Pascoe, D. and Richards, R. J., 1970.—“Variations in the respiratory quotient of the daughter sporocysts of Cercaria dichotoma Lebour, 1911 during starvation.” Acta parasit. pot., 18, 107–114.Google Scholar
Pascoe, D., and Richards, R. J. and James, B. L., 1908.—“Oxygen uptake, metabolic rate, reduced weight, length and number of cercariae in starving sporocysts of Cercaria dichotoma.” Expl Parasit., 23, 171–182.Google Scholar
Pascoe, D., and Richards, R. J., and James, B. L., 1970.—“The survival of the daughter sporo- cysts of Microphallus pygmaeus (Levinsen, 1881) Trcmatoda: Micro- phallidae) in a chemically defined medium.” The Veliger, 13, 157–102.Google Scholar
Richards, R. J., 1969a.—“Variations in the oxygen uptake, reduced weight, metabolic rate and length of the daughter sporocysts of Microphallus pygmaeas in a chemically defined medium.” Ph.D. Thesis, University College, Swansea, Chap. 4.Google Scholar
Richards, R. J., 1969b.—“Qualitative and quantitative estimations of the free amino acids in the parasitised and healthy digestive gland and gonad of Littorina saxatilis tenebrosa (Mont.) and in the daughter sporocysts of Microphallus pygmaeus (Levinsen, 1881) and Microphallus similis (Jfigerskiold, 1900) (Trematoda: Microphallidae).” Comp. Biochem. Physiol., 35, 655–665.Google Scholar
Richards, R. J., 1970a.—“The effect of starvation in vitro, on the free amino acids and sugars in the daughter sporocysts of Microphallus pygmaeus (Levinsen, 1881) (Trematoda: Microphallidae).” Z. ParasitenKde, 35, 31–39.Google Scholar
Richards, R. J., 1970b.—“The leakage and transamination of amino acids in vitro, by the germinal sacs of Digenea from marine molluscs.” J. Helminth., 44, 231–241.CrossRefGoogle Scholar
Richards, R. J., 1970c.—“Variations in the oxygen uptake, reduced weight and metabolic rate of starving sporocysts of Microphallus pygmaeus (Levinsen, 1881) (Trematoda: Microphallidae).” J. Helminth., 44, 75–88.Google Scholar
Richards, R. J., Pascoe, D. and James, B. L., 1970.—“Variations in the metabolism of the daughter sporocysts of Cercaria dichotoma Lebour, 1911, containing metacercariae, during starvation in sea water and in L-glutamine and glucose solutions.” Acta parasit. pot., 18, 347–357.Google Scholar
Smyth, J. D., 1969.—“Parasites as biological models.” Parasitology, 59, 73–92.CrossRefGoogle ScholarPubMed
Watts, S. D. M., 1970a.—“The amino acid requirements of the rediae of Crypto- cotyle lingua and Himasthla Uptosoma and of the sporocysts of Cercaria emasculans Pelseneer, 1900.” Parasitology, 61, 491–497.CrossRefGoogle Scholar
Watts, S. D. M., 1970b.—“Transamination in homogenates of rediae of Cryptocotyle lingua and of sporocysts of Cercaria emasculans Pelseneer, 1900.” Parasitology, 61, 499–504.Google Scholar
Zeuthen, E., 1948.—“A Cartesian diver balance weighing reduced weights (R.W.) with an accuracy of ± 0·01 μgm.” C. r. Trav. Lab. Carlsberg Ser. chim., 26, 243–266.Google Scholar
Zeuthen, E., 1950.—“The Cartesian diver respirometer.” Biol. Bull. mar. biol. Lab., Woods Hole. 98, 139–143.CrossRefGoogle ScholarPubMed
Zeuthen, E., 1953.—“Oxygen uptake as related to body size in organisms.” Q. Rev. Biol., 28, 1–12.Google Scholar