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Use of mitochondrial RNA genes for the differentiation of four Trichinella species by multiplex PCR amplification

Published online by Cambridge University Press:  01 June 2009

R. Blaga ,
BaoQuan Fu ,
D. Le Rhun ,
E. Le Naour ,
A. Heckman ,
A. Zocevic ,
MingYuan Liu  and
P. Boireau
R. Blaga*
Affiliation:
University of Agricultural Sciences and Veterinary Medicine, 400372Cluj-Napoca, Romania
BaoQuan Fu
Affiliation:
Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046Lanzhou, P.R. China
D. Le Rhun
Affiliation:
JRU BIPAR INRA AFSSA ENVA UPVM, 94703Maisons-Alfort cedex, France
E. Le Naour
Affiliation:
JRU BIPAR INRA AFSSA ENVA UPVM, 94703Maisons-Alfort cedex, France
A. Heckman
Affiliation:
JRU BIPAR INRA AFSSA ENVA UPVM, 94703Maisons-Alfort cedex, France
A. Zocevic
Affiliation:
JRU BIPAR INRA AFSSA ENVA UPVM, 94703Maisons-Alfort cedex, France
MingYuan Liu
Affiliation:
Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, 130062Changchun, P.R. China
P. Boireau
Affiliation:
JRU BIPAR INRA AFSSA ENVA UPVM, 94703Maisons-Alfort cedex, France
*
*E-mail: [email protected]
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Abstract

Until now, four species of the Trichinella genus have been identified in Europe: Trichinella spiralis, T. nativa, T. britovi and T. pseudospiralis. The aim of this work was to establish a sound polymerase chain reaction (PCR)-based method to differentiate these four species using mitochondrial rDNA as a reliable genetic marker and to evaluate the sensitivity of this method. Full-length DNA sequences coding for the small and large mitochondrial rRNA (mt-rrnS and mt-rrnL) of the four species are described. A multiplex PCR was designed and successfully tested on 24 European isolates. As few as one larva, or 100 pg of genomic DNA was detected, providing equivalent sensitivity to previously described PCR methods. The PCR-based method of mitochondrial rDNA amplification was thereby established as a sensitive and reproductive diagnostic method for the four European Trichinella species.

Type
Research Papers
Information
Journal of Helminthology , Volume 83 , Special Issue 2: Symposium and related papers from the 10th European Multicolloquium of Parasitology , June 2009 , pp. 121 - 128
Copyright
Copyright © Cambridge University Press 2009

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