Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-05T21:04:57.722Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of an aceto-carmine procedure to examine the excysted metacercariae of Echinostoma caproni and E. trivolvis

Published online by Cambridge University Press:  05 June 2009

B. Fried  and
P. M. Manger Jr
B. Fried
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
P. M. Manger Jr
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
Get access Rights & Permissions [Opens in a new window]

Abstract

A simple aceto-carmine procedure was developed to relax, fix, stain, and clear excystcd metacercariae of Echinostoma caproni and E. trivolvis. This procedure allowed for morphologic details on whole metacercariae comparable to those seen with more elaborate staining procedures. Differences in dimensions and staining intensities between the two species of metacercariae are described.

Keywords

techniquesaceto-carminestainingexcysted metacercariaeTrematodaEchinosToma caproniEchinostoma trivolvis
Type
Research Note
Information
Journal of Helminthology , Volume 66 , Issue 3 , September 1992 , pp. 238 - 240
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Fried, B. & Emili, S. (1988) Excystation in vitro of Echinostoma liei and E. revolutum (Trematoda) metacercariae. Journal of Parasitology 74, 98–102.CrossRefGoogle Scholar
Gower, W. C. (1939) A modified stain and procedure for trematodes. Stain Technology 14, 31–32.CrossRefGoogle Scholar
Webb, S. C. (1991) Use of acetic orcein as a fast method to relax, fix, clear and stain cercariae for light microscopy. Journal of Helminthology 65, 234–238.CrossRefGoogle ScholarPubMed