Hostname: page-component-848d4c4894-4rdrl Total loading time: 0 Render date: 2024-07-05T14:35:09.306Z Has data issue: false hasContentIssue false
  • English
  • Français

Ultrastructural study on spermatogenesis in Ganeo tigrinum, an intestinal trematode of Rana tigrina

Published online by Cambridge University Press:  05 June 2009

P.N. Sharma  and
N. Rai
P.N. Sharma
Affiliation:
Department of Zoology, Mohanlal Sukhadia University, Udaipur 313001, India
N. Rai
Affiliation:
Department of Zoology, Mohanlal Sukhadia University, Udaipur 313001, India
Get access Rights & Permissions [Opens in a new window]

Abstract

Spermatogenesis and the structure of the spermatozoon of the frog intestinal fluke, Ganeo tigrinum has been examined by electron microscopy. Each testis is covered by a tunica consisting of sustentacular cells and fibrous material. Internally the testis is lined by undifferentiated spermatogonial cells, with cytoplasm rich in free ribosomes. The arrangement of the diverse spermatogonic cell populations is similar to those studied in other digeneans. During spermatogenesis the elongation and spiralling of nuclear materials occurs synchronously in the late spermatid stage. The G. tigrinum spermatozoon appears to be biflagellate due to the presence of two filaments. Between the two filaments is an elongated nucleus; these elements fuse to form the monopartite shaft of the sperm. The spermatozoon of G. tigrinum is characterized by the presence of an unusually long nucleus that even surpasses the length of the mitochondrion. At almost half the length of the spermatozoon there is sharp rotation between first axial filament, mitochondrion and the nucleus. Both the axial filaments show 9+1 arrangement of axonemal units throughout the spermatozoon.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 1 , March 1995 , pp. 77 - 84
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Burton, P.R. (1960) Gametogenesis and fertilization in frog lung fluke, Haematoloechus medioplexus (Trematoda: Plagiorchiidae). Journal of Morphology 107, 93–122.CrossRefGoogle ScholarPubMed
Castilho, F. & Barandela, T. (1990) Ultrastructural study on spermatiogenesis and spermatozoon of the metacercariae of Microphallus primus (Digenea), a parasite of Carcinus maenas. Molecular Reproduction and Development 25, 140–146.CrossRefGoogle Scholar
Daddow, L.Y.M. & Jamieson, B.G.M. (1983) An ultra-structural study of spermiogenesis in Neochasmus sp. (Cryptogonimidae: Digenea: Trematoda). Australian Journal of Zoology 31, 1–14.CrossRefGoogle Scholar
Friend, D.S. & Farquhar, N.G. (1967) Functions of coated vesicles during protein absorption in the rat vas deferens. Journal of Cell Biology 35, 357–376.CrossRefGoogle ScholarPubMed
Fujino, T., Ishii, Y. & Mori, T. (1977) Ultrastructural studies on the spermatozoa and spermatogenesis in Paragonimus and Eurytrema (Trematoda: Digenea). Japanese Journal of Parasitology 26, 240–255.Google Scholar
Grant, W.C., Harkema, R. & Muse, K.E. (1976) Ultrastructure Pharnyogstomides procynois Harkema, 1942 (Diplostomatidae) 1. Observations on the male reproduction system. Journal of Parasitology 62, 39–49.CrossRefGoogle Scholar
Gresson, R.A.R. & Perry, M.M. (1961) Electron microscope studies of spermatoleosis in F. hepatica L. Experimental Cell Research 22, 1–8.CrossRefGoogle Scholar
Halton, D.W. & Hardcastle, A. (1976) Spermatogenesis in a monogenean, Diclidophora merlangi. International Journal for Parasitology 6, 43–53.CrossRefGoogle Scholar
Hendow, H.T. & James, B.L. (1988) Ultrastructure of spermatozoon and spermatogenesis in Maritrema linguilla (Digenea; Microphallidae). International Journal for Parasitology 18, 53–63.CrossRefGoogle ScholarPubMed
Justine, J.L. & Mattei, X. (1984) A typical spermiogenesis in a parasitic flatworm Didymozoon (Trematoda: Digenea: Didymozoidae). Journal of Ultrastructural Research 87, 106–111.CrossRefGoogle Scholar
Kitajima, E.W., Paraense, W.H. & Correa, L.R. (1976) The fine structure of Schistosoma mansoni sperm (Trematoda: Digenea). Journal of Physiology 62, 215–221.Google ScholarPubMed
Otubanjo, O.A. (1981) Schistosoma mansoni: The sustentacular cells of testes. Parasitology 82, 125–130.CrossRefGoogle ScholarPubMed
Rees, F.G. (1979) The ultrastructure of the spermatozoon and spermiogenesis in Cryptocotyle lingua (Digenea: Heterophyidae). International Journal for Parasitology 9, 405–419.CrossRefGoogle Scholar
Robinson, R.D. & Halton, D.W. (1982) Fine structural observations on spermatogenesis in Corrigia vitta (Trematoda: Dicrocoeliidae) Zeitschrift für Parasitenkunde 68, 53–72.CrossRefGoogle Scholar
Roth, T.F. & Porter, K.R. (1964) Yolk protein uptake in the oocyte of the mosquito Aedes aegypti L. Journal of Cell Biology 28, 313–332.CrossRefGoogle Scholar
Sato, M., Oh, M. & Sakoda, K. (1967) Electron microscopic study of spermatogenesis in lung fluke (Paragonimus miyazakii). Zeitschrift für Zellforsch und Mikrosropische Anatomie 77, 232–243.CrossRefGoogle ScholarPubMed
Shapiro, J.E., Hershenov, B.R. & Tulloch, G.S. (1961) The fine structure of Haematoloechus spermatozoon tail. Journal of Biophysics, Biochemistry and Cytology 9, 211–217.CrossRefGoogle Scholar
Sharma, A.N. & Sharma, P.N. (1980) Chemoarchitectural characteristics of germ cells during spermatogenesis in Ceylonocotyle scoliocoelium (Trematoda: Digenea). Indian Journal of Experimental Biology 18, 1282–1287.Google Scholar
Zdarska, Z., Soboleva, T.N., Sterba, J. & Valkounova, J. (1991) Ultrastructure of the male reproductive system of the trematode Brachylaimus aequans. Folia Parasitologica 38, 33–37.Google ScholarPubMed