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Ultrastructural study of Trichinella spiralis with emphasis on adult female reproductive organs

Published online by Cambridge University Press:  05 June 2009

Y. Takahashi ,
C. Goto  and
K.K. Kita
Y. Takahashi
Affiliation:
Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa, Gifu 500, Japan
C. Goto
Affiliation:
Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa, Gifu 500, Japan
K.K. Kita
Affiliation:
Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa, Gifu 500, Japan
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Abstract

The ultrastructure of the reproductive organs of adult female T. spiralis was described on the basis of extensive observation under an electron microscope. The organisms were recovered from the host intestine 7 days after oral infection. The organs consisted of a single ovary, seminal receptacle, uterus and vagina, confirming the reports described by previous authors at light microscopical level. All the organs were surrounded by a basal lamina and epithelial cells, and were bathed in haemolymph. Ova were formed in a germinal zone on the ovary wall exhibiting a half-moon shape, and spread to other sides of the ovary Mature ova exhibited a smooth cell surface, cuboidal shape, prominent cytoplasmic polysomes, a clear nucleus and a well-developed nucleolus. Embryos in early stage of development consisted of numerous small cells and were surrounded by a sheath.As they matured they shed the sheath and left a pool of sheaths in the uterus. During oogenesis and embryogenesis of adult worms in normal development, there occurred lipid droplets and the degradation of embryos; reportedly signs of worm damage.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 3 , September 1995 , pp. 247 - 252
Copyright
Copyright © Cambridge University Press 1995

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References

Appleton, J.A., Bell, R.G., Homan, W. & van Knapen, F. (1991) consensus on Trichinella spiralis antigens and antibodies. Parasitology Today 7, 190–192.CrossRefGoogle Scholar
Bell, R.G. & McGregor, D.D. (1979) Trichinella spiralis: role of different life cycle phases in induction, maintenance, and expression of rapid expulsion in rats. Experimental Parasitology 48, 51–60.CrossRefGoogle ScholarPubMed
Berntzen, A.K. (1965) Comparative growth and development of Trichinella spiralis in vitro and in vivo, with a redescription. Experimental Parasitology 16, 74–106.CrossRefGoogle ScholarPubMed
Fuhrman, J.A., Lane, W.S., Smith, R.F. & Piessens, W.F. (1992) Transmission-blocking antibodies recognize microfilarrial chitinase in brugian lymphatic filariasis. Proceedings of the National Academy of Sciences, USA 8, 1548–1552.CrossRefGoogle Scholar
Love, R.J., Ogilvie, B.M. & Mclaren, D.J. (1976) The immune mechanism which expels the intestinal stage of Trichinella spiralis from rats. Immunology 30, 7–15.Google ScholarPubMed
Maizels, R.M., Blaxter, M.L. & Selkirk, M.E. (1993) Form and function of nematode surfaces. Experimental Parasitology 77, 380–384.CrossRefGoogle Scholar
Rothwell, T.L.W. (1989) Immune expulsion of parasitic nematodes from the alimentary tracts.International Journal for Parasitology 19, 139–168.CrossRefGoogle Scholar
Silberstein, D.S. & Despommier, D.D. (1984) Antigens from Trichinella spiralis that induce a protective response in the mouse. Journal of Immunology 132, 898–904.CrossRefGoogle ScholarPubMed
Silberstein, D.S. & Despommier, D.D. (1985a) Effects on Trichinella spiralis of host response to purified antigens. Science 227, 948–950.CrossRefGoogle ScholarPubMed
Silberstein, D.S. & Despommier, D.D. (1985b) Immunization with purified antigens protects mice from lethal infection with Trichinella spiralis. Journal of Parasitology 71, 516–517.CrossRefGoogle ScholarPubMed
Takahashi, Y., Uno, T., Mizuno, N., Aisaka, A., Yagi, J. & Araki, T. (1990) Immunocytochemical evaluationof Trichinella spiralis larval antigen. Journal of Electron Microscopy 39, 155–159.Google Scholar
Takahashi, Y.Homan, W.&Lim, P.L. (1993) Ultrastructual localization of the phosphoryIcholine-associated antigen in Trichinella spiralis. Journal of Parasitology 79, 604–609.CrossRefGoogle Scholar
Takahashi, Y., Mizuno, N., Araki, T., Okuda, H. & Nakashima, T. (1994) Immunocytochemical localization of antigens in adults worms of Trichinella spiralis recognized by Fischer rats. Parasitology Research 80, 291–296.CrossRefGoogle ScholarPubMed
Takahashi, Y., Goto, C. & Kita, K.K. (1994) Ultrastructural study of Trichinella spiralis with emphasis on adult male reproductive organs. Journal of Helminthology 68, 353–358.CrossRefGoogle ScholarPubMed
Wakelin, D. & Denham, D.A. (1983) The immune response. p. 265 in Campbell, W.C. (Ed.) Trichinella spiralis and trichinosis. New York, Plenum Press.CrossRefGoogle Scholar
Wu, L.Y. (1955) Studies onTrichinella spiralis I. Male and female reproductive systems. Journal of Parasitology 41, 40–47.CrossRefGoogle Scholar