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Trichrome staining of Gyrodactylus sclerites and soft tissues following fixation in ammonium picrate-glycerin, including an improved rendition of the haptoral bars of G. turnbulli

Published online by Cambridge University Press:  05 June 2009

G.R. Richards  and
J.C. Chubb
G.R. Richards
Affiliation:
Department of Environmental and Evolutionary Biology, University of Liverpool, Derby Building, PO Box 147, Liverpool, L69 3 BX, UK
J.C. Chubb
Affiliation:
Department of Environmental and Evolutionary Biology, University of Liverpool, Derby Building, PO Box 147, Liverpool, L69 3 BX, UK
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Abstract

A simple technique using modified Mallory stain in the transferral of Gyrodactylus specimens from ammonium picrate-glycerin to a permanent mountant is described. Hamuli, their connecting bars and the penis sclerites are well defined by the technique as are muscles and tendons, cell nuclei, tegument and gland cells. As well as being useful in the study of general anatomy, the technique enhances the observation of the taxonomically important ventral and dorsal bars. In order to show this, improved illustrations of the dorsal and ventral bars of G. turnbulli are given along with explicit demonstrations of differences in morphology of the ventral bars of G. bullatarudis and G. rasini - two easily confused species.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 2 , June 1995 , pp. 149 - 154
Copyright
Copyright © Cambridge University Press 1995

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References

An, L, Jara, C.A. & Cone, D.K. (1991) Five species of Gyrodactylus Nordmann 1832 (Monogenea) from freshwater fishes of Peru. Canadian journal of Zoology 69,1199–1202.CrossRefGoogle Scholar
Bradbury, P. & Gordon, K.C. (1982) Connective tissue and stains, pp. 122–144 in Bancroft, J.D. & Stevens, A. (Eds) Theory and practice of histological techniques. 2nd edn.Edinburgh, London, Melbourne and New York, Churchill Livingstone.Google Scholar
Ergens, R. (1965a) Die morphogenese der chitenoiden Teile des Haptors bei Gyrodactylus decorus Malmberg, 1956 (Monogenoidea) und ihre morphologisch-metrische Variabilität. Zeitschrift für Parasitenkunde 25, 359–370.CrossRefGoogle Scholar
Ergens, R. (1965b) Die morphogenese der chitenoiden Teile des Haptors bei Gyrodactylus tincae (Malmberg, 1956) Malmberg, 1964 (Monogenoidea) und ihre morpholigisch- metrische Variabilität. Zeitschrift für Parasitenkunde 26, 173–184.CrossRefGoogle Scholar
Ergens, R. (1969) The suitability of ammonium picrateglycerin in preparing slides of lower Monogenoidea. Folia Parasitologica 16, 320.Google Scholar
Ergens, R. (1983) A survey of the results of studies on Gyrodactylus katherineri Malmberg, 1964 (Gyrodactylidae: Monogenea). Folia Parasitologica 30, 319–327.Google Scholar
Harris, P.D. (1986) Species of Gyrodactylus von Nordmann, 1832 (Monogenea Gyrodactylidae) from poeciliid fishes, with a description of G. turnbulli sp. nov. from the guppy, Poecilia reticulata Peters. Journal of Natural History 20, 183–191.CrossRefGoogle Scholar
Kayton, RJ. (1983) Histochemical and X-ray elemental analysis of the sclerites of Gyrodactylus spp. (Platyhelminthes: Monogenoidea) from the Utah chub, Gila atraria (Girard). Journal of Parasitology 69, 862–865.CrossRefGoogle Scholar
Kritsky, D.C., Leiby, P.D. & Kayton, R.J. (1978) A rapid stain technique for haptoral bars of Gyrodactylus species (Monogenea). Journal of Parasitology 64, 172–174.CrossRefGoogle ScholarPubMed
Lyons, K.M. (1966) The chemical nature and evolutionary significance of monogenean attachment sclerites. Parasitology 56, 63–100.CrossRefGoogle ScholarPubMed
Malmberg, G. (1956) Om förekomsten av Gyrodactylus på svenska fiskar. Skrifter utigivna av Södra Sveriges Fiskeriforening Êrsskrift 1956, 19–76.Google Scholar
Malmberg, G. (1970) The excretory systems and the marginal hooks as a basis for the systematics of Gyrodactylus. Arkiv för Zoologi 23, 1–273.Google Scholar
Mo, T.A. & Appleby, C. (1990) A special technique for studying haptoral sclerites of monogeneans. Systematic Parasitology 17, 103–108.CrossRefGoogle Scholar
Reuter, M. (1987) Immunocytochemical demonstration of serotonin and neuropeptides in the nervous system of Gyrodactylus salaris (Monogenea). Acta zoologica 68, 187–193.CrossRefGoogle Scholar
Shinn, A.P., Gibson, D.I. & Sommerville, C. (1993) An SEM study of the haptoral sclerites of the genus Gyrodactylus Nordmann, 1832 (Monogenea) following extraction by digestion and sonication techniques. Systematic Parasitology 25, 135–144.CrossRefGoogle Scholar
Zdárska, Z. (1976) Selective staining of opisthaptor sclerites of some monogeneans. Folia Parasitologica 23, 182.Google ScholarPubMed