Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-20T00:39:14.309Z Has data issue: false hasContentIssue false
  • English
  • Français

Trichinellosis survey in the wild boar from the Toledo mountains in south-western Spain (2007–2008): molecular characterization of Trichinella isolates by ISSR-PCR

Published online by Cambridge University Press:  01 June 2009

R.N. García-Sánchez ,
J.J. Nogal-Ruiz ,
R. Manzano-Lorenzo ,
J.M. Arroyo Díaz ,
G. Pérez López ,
F.J. Sánchez Ruano ,
A. Ramiro Casas ,
C. Crespo Bascón ,
F. Bolás-Fernández  and
A.R. Martínez-Fernández
R.N. García-Sánchez
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
J.J. Nogal-Ruiz
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
R. Manzano-Lorenzo
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
J.M. Arroyo Díaz
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
G. Pérez López
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
F.J. Sánchez Ruano
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
A. Ramiro Casas
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
C. Crespo Bascón
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
F. Bolás-Fernández*
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
A.R. Martínez-Fernández
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
*
*Fax: +34 91 394 18 15 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In Spain, trichinellosis represents a public health problem, with an average of five outbreaks per year, wild boar meat being the main source of infection. A trichinellosis survey (2007–2008 hunting campaign) was carried out on wild boars in the Toledo Mountains (south-western Spain, EU) in the context of a surveillance programme on wildlife diseases. A total of 2216 wild boars from different locations of the region were examined. The examination was carried out by veterinarians in the local abattoir (Matadero Municipal de Toledo). The positive samples were sent to the Department of Parasitology (Facultad de Farmacia, UCM) for experimental isolation and specific identification by inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR). Using this technique we identified 17 isolates as Trichinella spiralis with an electrophoretic profile indistinguishable from the T. spiralis reference strain (ISS48). We confirmed that ISSR-PCR is a robust technique for the molecular identification of Trichinella isolates. According to our results, the prevalence of T. spiralis in wild boars from the Toledo Mountains (>800 m above sea level) during the hunting season was approximately 0.77%. The prevalence of T. spiralis (100% of our observations) is a good example of the persistence of this species in sylvatic conditions (coming from the domestic cycle), if a good wild host is abundant. Our observations confirm the major prevalence of T. spiralis over T. britovi in this region, as well as the risk to human health represented by the consumption of uninspected wild boar meat.

Type
Research Papers
Information
Journal of Helminthology , Volume 83 , Special Issue 2: Symposium and related papers from the 10th European Multicolloquium of Parasitology , June 2009 , pp. 117 - 120
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cabaj, W. (2006) Wild and domestic animals as permanent Trichinella reservoirs in Poland. Wiadomosci Parazytologiczne 52, 175–179.Google ScholarPubMed
Casulli, A., La Rosa, G., Amati, M. & Pozio, E. (2001) High prevalence of Trichinella nativa infection in wolf (Canis lupus) populations of Tvier and Smoliensk regions of European Russia. Parasite 8 (2 Suppl.), S88–S89.CrossRefGoogle ScholarPubMed
EFSA (European Food Safety Authority) (2006a) Trends and sources of zoonoses, zoonotic agents and antimicrobial resistance in the European Union in 2004. Available at:http://www.efsa.europa.eu/en/science/monitoring_zoonoses/reports/1277.html (accessed 19 December 2007).Google Scholar
EFSA (European Food Safety Authority) (2006b) The community summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in the European Union in 2005. Available at:http://www.efsa.europa.eu/en/science/monitoring_zoonoses/reports/zoonoses_report_2005.html (accessed 19 December 2007).Google Scholar
Fonseca-Salamanca, F., Nogal-Ruiz, J.J., Benito, C., Camacho, M.V. & Martínez-Fernández, A.R. (2006) Molecular characterization of Trichinella genotypes by inter-simple sequence repeat polymerase chain reaction (ISSR-PCR). Journal of Parasitology 92, 606–610.CrossRefGoogle ScholarPubMed
Fonseca-Salamanca, F., Nogal-Ruiz, J.J., García-Sánchez, R.N., Bolas-Fernandez, F., Jiménez, S., Álamo, R., Gárate, T. & Martínez-Fernández, A.R. (2009) Prevalence of Trichinella spp. in North Spain wild fauna and new variety of Trichinella britovi identification. Veterinary Parasitology 159, 222–224.CrossRefGoogle ScholarPubMed
Hars, J., Rossi, S., Boue, F., Garin-Bastuji, B., Le Potier, M.F., Boireau, P., Hattenberger, A.M., Louguet, Y. & Toma, B. (2007) Le risque sanitaire lié au sanglier sauvage. Bulletin des Groupements Techniques Vétérinaires 40, 37–41.Google Scholar
Järvis, T., Kapel, Ch., Moks, E., Talvik, E. & Mägi, E. (2007) Helminths of wild boar in the isolated population close to the northern border of its habitat area. Veterinary Parasitology 150, 366–369.CrossRefGoogle Scholar
Pérez-Martín, J.E., Serrano, F.J., Reina, D., Mora, J.A. & Navarrete, I. (2000) Sylvatic trichinellosis in southwestern Spain. Journal of Wildlife Diseases 36, 531–534.CrossRefGoogle ScholarPubMed
Perteguer, M.J., Rodríguez, E., García-Sánchez, R.N., Nogal-Ruiz, J.J., Bolas-Fernández, F., Martínez-Fernández, A.R. & Gárate, T. (2009) Identification of Spanish Trichinella isolates by ISSR-PCR: Intra-specific variability of Trichinella britovi. Veterinary Parasitology 159, 206–209.CrossRefGoogle ScholarPubMed
Pozio, E. (1998) Trichinellosis in the European Union: epidemiology, ecology and economic impact. Parasitology Today 14, 35–38.CrossRefGoogle ScholarPubMed
Pozio, E. & Murrell, K.D. (2006) Systematics and epidemiology of Trichinella. Advances in Parasitology 63, 367–439.CrossRefGoogle ScholarPubMed
Pozio, E., Rinaldi, L., Marucci, G., Musella, V., Galati, F., Cringoli, G., Boireau, P. & La Rosa, G. (2009) Hosts and habitats of Trichinella spiralis and Trichinella britovi in Europe. International Journal for Parasitology 39, 71–79.CrossRefGoogle ScholarPubMed
Rafter, P., Marucci, G., Brangan, P. & Pozio, E. (2005) Rediscovery of Trichinella spiralis in red foxes (Vulpes vulpes) in Ireland after thirty years of oblivion. Journal of Infection 50, 61–65.CrossRefGoogle Scholar
Rodriguez de las Parras, E., Rodriguez-Ferrer, M., Nieto-Martínez, J., Ubeira, F.M. & Garate-Ormaechea, T. (2004) Trichinellosis outbreaks in Spain (1999–2001). Enfermedades Infecciosas y Microbiología Clínica 22, 70–76.Google Scholar
Rosenthal, B.M., La Rosa, G., Zarlenga, D., Dunams, D., Chunyu, Y., Mingyuan, L. & Pozio, E. (2008) Human dispersal of Trichinella spiralis in domesticated pigs. Infection Genetics and Evolution 8, 799–805.CrossRefGoogle ScholarPubMed
Zietkiewicz, E., Rafalski, A. & Labuda, D. (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176–183.CrossRefGoogle ScholarPubMed
Zimmer, I.A., Hunter, S.J., Morgan, C.P., Hunt, K.R., Smith, G.C., Howell, M. & Taylor, M.A. (2008) Detection and surveillance for animal trichinellosis in GB. Veterinary Parasitology 151, 233–241.CrossRefGoogle ScholarPubMed