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Toxocara canis infections in a pig model: immunological, haematological and blood biochemistry responses

Published online by Cambridge University Press:  12 April 2024

I.E. Sommerfelt ,
G. Santillán ,
G. Mira ,
M. Ribicich ,
A. Betti  and
R. De Torres
I.E. Sommerfelt*
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín 280 (1427), Buenos Aires, Argentina Cátedra de Veterinaria en Salud Pública, Instituto Nacional de Parasitología-ANLIS Dr Carlos G. Malbrán, Buenos Aires, Argentina
G. Santillán
Affiliation:
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología-ANLIS Dr Carlos G. Malbrán, Buenos Aires, Argentina
G. Mira
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín 280 (1427), Buenos Aires, Argentina
M. Ribicich
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín 280 (1427), Buenos Aires, Argentina
A. Betti
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín 280 (1427), Buenos Aires, Argentina
R. De Torres
Affiliation:
Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
*
*Fax: 54 11 45148969 E-mail: [email protected]
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Abstract

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The immunological, haematological and enzymatic responses to the inoculation in pigs of 100,000 embryonated eggs of Toxocara canis were studied. Fifteen females were inoculated and three remained as controls. Haematological values were analysed from day 7 p.i. until day 126 p.i. In the inoculated group, white blood cells were raised on day 14 p.i. and eosinophil values on days 7, 14, 21, 35 and 49 p.i. showing significant differences compared with controls (P<0.05). Absolute eosinophil counts (per ml) presented two rises, the first on days 7, 14 and 21 p.i. and the second on days 35 and 49 p.i. Blood biochemistry was maintained within normal values. Serological examination by ELISA to determine antibody levels against Toxocara canis L2/L3 excretory–secretory (ES) antigens showed values higher than the positive cut-off (1:32) from day 7 p.i. and until the end of the study on day 126 p.i., presenting two peaks: one on day 28 p.i. and the second covering days 49 to 56 p.i. Western blots of sera of inoculated animals presented, from day 7 p.i., two polypeptide bands of 55 and 70 kDa MW and, from day 56 p.i., an additional band of 120 kDa MW, all of which persisted until the end of the study. Immunological responses were sustained over time. No direct correlation was observed between the rise in eosinophils and antibody titres. To validate the conclusions, more studies are required on the polypeptide bands.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 1 , March 2006 , pp. 73 - 77
Copyright
Copyright © Cambridge University Press 2006

References

Anon. (1994) Relationship between epilepsy and tropical diseases. Epilepsia 35, 89–93.CrossRefGoogle Scholar
Boa, M.E., Kassuku, A.A., Willingham, A.L. III, Keyyu, J.D., Phiri, I.K. & Nansen, P. (2002) Distribution and density of cysticerci of Taenia solium by muscle groups and organs in naturally infected local finished pigs in Tanzania. Veterinary Parasitology 106, 155–164.CrossRefGoogle ScholarPubMed
Dorny, P., Phiri, I.K., Vercruysse, J., Gabriel, S., Willingham, A.L. III, Brandt, J., Victor, B., Speybroeck, N. & Berkvens, D. (2004) A Bayesian approach for estimating values for prevalence and diagnostic test characteristics of porcine cysticercosis. International Journal for Parasitology 34, 569–576.CrossRefGoogle ScholarPubMed
Engels, D., Urbani, C., Belotto, A., Meslin, F. & Savioli, L. (2003) The control of human (neuro) cysticercosis: which way forward? Acta Tropica 87, 177–182.CrossRefGoogle ScholarPubMed
Gonzalez, A.E., Cama, V., Gilman, R.H., Tsang, V.C.W., Pilcher, J.B., Chavera, A., Castro, M., Montenegro, T., Verastegui, M., Miranda, E. & Bazalar, H. (1990) Prevalence and comparison of serological assays, necropsy, and tongue examination for the diagnosis of porcine cysticercosis in Peru. American Journal of Tropical Medicine and Hygiene 43, 194–199.CrossRefGoogle ScholarPubMed
Gracey, J.F. (Ed.) (1986) Parasitic diseases. pp. 391–393 in Meat hygiene, 8th edn. English Language Book Society/Bailliere, UK.Google Scholar
Nguekam, J.P., Zoli, A.P., Vondou, L., Pouedet, S.M.R., Assana, E., Dorny, P., Brandt, J. & Geerts, S. (2003) Kinetics of circulating antigens in pigs experimentally infected with Taenia solium eggs. Veterinary Parasitology 111, 323–332.CrossRefGoogle ScholarPubMed
Onah, D.N. & Chiejina, S.N. (1995) Taenia solium cysticercosis and human taeniosis in the Nsuka area of Enugu State, Nigeria. Annals of Tropical Medicine and Parasitology 89, 399–407.CrossRefGoogle Scholar
Phiri, I.K., Ngowi, H., Afonso, S., Matenga, E., Boa, M., Mukaratirwa, S., Githigia, S., Saimo, M., Sikasunge, C., Maingi, N., Lubega, G.W., Kassuku, A., Michael, L., Siziya, S., Krecek, R.C., Noormahomed, E., Vilhena, M., Dorny, P. & Willingham, A.L. III (2003) The emergence of Taenia solium cysticercosis in eastern and southern Africa as a serious agricultural problem and public health risk. Acta Tropica 87, 13–23.CrossRefGoogle ScholarPubMed
Sciutto, E., Martínez, J.J., Villalobos, N.M., Hernández, M., José, M.V., Beltrán, C., Rodarte, F., Flores, I., Bobadilla, J.R., Fragoso, G., Parkhouse, M.E., Harrison, L.J.S. & De Aluja, A.S. (1998) Limitations of current diagnostic procedures for the diagnosis of Taenia solium cysticercosis in rural pigs. Veterinary Parasitology 79, 299–313.CrossRefGoogle ScholarPubMed