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Tests on the centrifugal flotation technique and its use in estimating the prevalence of Toxocara in soil samples from urban and suburban areas of Malaysia

Published online by Cambridge University Press:  05 June 2009

A.G. Loh  and
D.A. Israf
A.G. Loh
Affiliation:
Faculty of Veterinary Medicine and Animal Science, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
D.A. Israf*
Affiliation:
Faculty of Veterinary Medicine and Animal Science, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
*
* Fax: 603 9430626 E-mail: [email protected]
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Abstract

The influence of soil texture (silt, sand and laterite) and flotation solutions (saturated NaCl, sucrose, NaNO3 and ZnSO4) upon the recovery of Toxocara ova from seeded soil samples with the centrifugal flotation technique was investigated. Soil samples of different texture were artificially seeded with Toxocara spp. ova and subjected to a centrifugal flotation technique which used various flotation solutions. The results showed significant (P<0.001) interactions between the soil types and the flotation solutions. The highest percentage of ova recovery was obtained with silty soil (34.9−100.8%) with saturated NaC1 as the flotation solution (45.3−100.8%). A combination of washing of soil samples with 0.1% Tween 80, and flotation using saturated NaCl and a 30 min coverslip recovery period was used to study the prevalence of contamination of soil samples. Forty-six soil samples were collected from up to 24 public parks/playgrounds in urban areas of Petaling Jaya and suburban areas of Serdang. The prevalence of Toxocara species in the urban and suburban areas was 54.5% and 45.8% respectively.

Type
Research Papers
Information
Journal of Helminthology , Volume 72 , Issue 1 , March 1998 , pp. 39 - 42
Copyright
Copyright © Cambridge University Press 1998

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