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Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom

Published online by Cambridge University Press:  19 March 2013

J. Canevari ,
L. Ceballos ,
R. Sanabria ,
J. Romero ,
F. Olaechea ,
P. Ortiz ,
M. Cabrera ,
V. Gayo ,
I. Fairweather  and
C. Lanusse
...Show all authors
J. Canevari
Affiliation:
Laboratorio de Farmacología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, UNCPBA, Campus Universitario, 7000Tandil, Argentina
L. Ceballos
Affiliation:
Laboratorio de Farmacología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, UNCPBA, Campus Universitario, 7000Tandil, Argentina
R. Sanabria
Affiliation:
Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE), Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata, 7130Chascomús, Argentina
J. Romero
Affiliation:
Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE), Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata, 7130Chascomús, Argentina
F. Olaechea
Affiliation:
Grupo de Salud Animal, INTA Bariloche, Rio Negro, CC 277, 8400Bariloche, Argentina
P. Ortiz
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional de Cajamarca, Cajamarca, Perú
M. Cabrera
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional de Cajamarca, Cajamarca, Perú
V. Gayo
Affiliation:
Instituto DILAVE, ‘Miguel C. Rubino’, Montevideo, Uruguay
I. Fairweather
Affiliation:
Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biological Centre, The Queen's University of Belfast, 97 Lisburn Road, BelfastBT97BL, United Kingdom
C. Lanusse
Affiliation:
Laboratorio de Farmacología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, UNCPBA, Campus Universitario, 7000Tandil, Argentina
L. Alvarez*
Affiliation:
Laboratorio de Farmacología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, UNCPBA, Campus Universitario, 7000Tandil, Argentina
*
*Fax: 54–249-4439850 E-mail: [email protected]
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Abstract

The main goal of the current work was to develop and validate an in vitro fluke egg hatch test, as a method for the detection of albendazole (ABZ) resistance in the liver fluke, Fasciola hepatica. Fluke eggs (200/ml, n= 5) from six different isolates were used in the current experimental work. They were obtained from different geographical locations and named Cullompton (UK), CEDIVE (Chascomus, Argentina), INTA-Bariloche (Bariloche, Argentina), Rubino (Uruguay), Cajamarca (Perú) and Río Chico (Catamarca, Argentina). The fluke eggs were incubated (25°C) for a 12-h period in the presence of either ABZ or its sulphoxide metabolite (ABZ.SO) (5, 0.5 or 0.05 nmol/ml). Untreated eggs were incubated as a control. Incubated eggs (with or without drug present) were kept in darkness at 25°C for 15 days. Afterwards, the trematode eggs were exposed to daylight over a 2-h period. Hatched and unhatched eggs were evaluated using an optical microscope, and the ovicidal activity was assessed for each fluke isolate. A very low ovicidal activity ( ≤ 13.4%) was observed in the ABZ-resistant CEDIVE isolate for both ABZ and ABZ.SO. Conversely, in the INTA-Bariloche and Río Chico isolates, which are suspected to be susceptible to ABZ, ovicidal activities ≥ 70.3% were observed after incubation with ABZ at the lowest concentration tested (0.05 nmol/ml). This finding correlates with that previously described for the ABZ-susceptible Cullompton. Finally, the Cajamarca and Rubino isolates behaved as ABZ resistant, since no ovicidal activity was observed after eggs were incubated with ABZ at 0.5 nmol/ml. Considering the specific results obtained for each isolate under assessment, the egg hatch test described here may be a suitable method for detection of ABZ resistance in F. hepatica.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 3 , September 2014 , pp. 286 - 292
Copyright
Copyright © Cambridge University Press 2013 

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