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Techniques for locating isotopically labelled schistosomula of Schistosoma mansoni in host tissues for ultrastructural investigations

Published online by Cambridge University Press:  18 November 2009

Jean E. Crabtree  and
R. A. Wilson
Jean E. Crabtree*
Affiliation:
Department of Biology, University of York, York YO1 5DD, UK.
R. A. Wilson
Affiliation:
Department of Biology, University of York, York YO1 5DD, UK.
*
*Department of Medicine, St. James' University Hospital, Leeds, LS9 7TF.
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Abstract

The use of 75Selenomethionine labelled cercariae of Schistosoma mansoni for ultrastructural localization of parasites in host tissues has been evaluated. Both gamma counting of tissue, and autoradiography of resin-embedded tissue were successful. The autoradiographic technique was more sensitive and schistosomula were readily located in pulmonary tissue up to 24 days post-infection.

Keywords

SchistosomaMansoniAutoradiographyElectronMicroscopy.
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 1 , March 1986 , pp. 75 - 78
Copyright
Copyright © Cambridge University Press 1986

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References

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