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Susceptibility to praziquantel of male and female cercariae of praziquantel-resistant and susceptible isolates of Schistosoma mansoni

Published online by Cambridge University Press:  18 September 2009

Y.-S. Liang ,
W. Wang ,
J.-R. Dai ,
H.-J. Li ,
Y.-H. Tao ,
J.-F. Zhang ,
W. Li ,
Y.-C. Zhu ,
G.C. Coles  and
M.J. Doenhoff
Y.-S. Liang
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
W. Wang
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
J.-R. Dai*
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
H.-J. Li
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
Y.-H. Tao
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
J.-F. Zhang
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
W. Li
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
Y.-C. Zhu
Affiliation:
Jiangsu Institute of Parasitic Diseases, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Meiyuan, 117 Yangxiang, Wuxi214064, Jiangsu Province, China
G.C. Coles
Affiliation:
Department of Clinical Veterinary Science, University of Bristol, Langford, BristolBS40 5DU, UK
M.J. Doenhoff
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK
*
*Fax: +86 510 85510263 E-mail: [email protected]
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Abstract

Praziquantel (PZQ) is now widely used for the treatment of human schistosomiasis. However, in recent years, there has been a growing concern about the resistance of Schistosoma to PZQ. The mechanisms of PZQ action against Schistosoma and resistance of Schistosoma to PZQ are poorly understood. Here, we report differential susceptibilities to PZQ between male and female cercariae in the PZQ-susceptible and PZQ-resistant isolates of Schistosoma mansoni, using tail loss as a measurement of PZQ action. The miracidia were collected by hatching eggs collected from faeces of infected mice. Single-sex cercaria lines were made by infecting a single Biomphalaria glabrata snail with a single miracidium. The sex of each single-sex cercaria line was identified by a direct W1-specific polymerase chain reaction (PCR) technique. Single-sex cercariae of two isolates were exposed to four different concentrations of PZQ, respectively. The tail shedding of cercariae was observed under a dissecting microscope for five time points up to 100 min after adding PZQ. The results showed that male cercariae have higher tail-shedding rates than that of female cercariae when PZQ-susceptible isolates of S. mansoni are exposed to the same concentration of PZQ. But this phenomenon was not observed in the PZQ-resistant isolates. This sexual differential resistance phenomenon of S. mansoni suggests that resistance to PZQ is induced by decreasing the PZQ susceptibility of male worms. The experiment described here may also be useful for developing tests to detect PZQ resistance in the field.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 2 , June 2010 , pp. 202 - 207
Copyright
Copyright © Cambridge University Press 2009

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