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Surface carbohydrates of Eudiplozoon nipponicum pre- and post-fusion

Published online by Cambridge University Press:  12 April 2024

I. Schabussova ,
B. Koubková ,
M. Gelnar ,
M. Schabuss  and
P. Horák
I. Schabussova*
Affiliation:
Department of Marine Biology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria
B. Koubková
Affiliation:
Department of Zoology and Ecology, Masaryk University, Kotlárská 2, 61137, Brno, Czech Republic
M. Gelnar
Affiliation:
Department of Zoology and Ecology, Masaryk University, Kotlárská 2, 61137, Brno, Czech Republic
M. Schabuss
Affiliation:
Department of Limnology, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria
P. Horák
Affiliation:
Department of Parasitology, Charles University, Vinicná 7, 128 44, Prague, Czech Republic
*
*Author for correspondence Fax: +431 42779542 Email: [email protected]
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Abstract

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The development of the monogenean Diplozoon (Nordmann, 1832) (Diplozoidae) necessitates fusion of two larval stages (diporpae) into one double organism. How diporpae find, distinguish and contact each other is unclear, nor is the nature of the stimuli responsible for the dedifferentiation of cells and the formation of new tissues at the site of somatic fusion. Previous studies have implied a role for carbohydrates and glycoproteins in the interactions between helminth parasites and their hosts. Hypothetically, glycoconjugates may also be involved in the establishment of parasite–parasite associations. Changes in the surface saccharide residues during the development of Eudiplozoon nipponicum, a gill ectoparasite of carp (Cyprinus carpio) are described. Flat-fixed specimens and sections of diporpae, juveniles (just-fused) and adult worms were examined following exposure to a panel of 12 FITC-conjugated lectins. All developmental stages exhibited a specific surface binding pattern with ten lectins, indicating that Man/Glc, GlcNAc, Gal and GalNAc are probably present on their surfaces. No reaction was observed with Fuc-specific lectins (UEA-I and LTA). There is evidence that parasite development is accompanied by both qualitative and quantitative changes in the saccharide pattern distribution. The diporpa sucker reacted with nine lectins, excluding BS-II. A very strong binding of PNA, LCA and ConA (Gal and Man/Glc-specific lectins) was observed with the papilla glands of juvenile worms. The role of glandular secretions in this unique fusion process is discussed.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 1 , March 2004 , pp. 63 - 68
Copyright
Copyright © Cambridge University Press 2004

References

Borgonie, B., Van Driessche, E., Link, C.D., De Waele, D. & Coomans, A. (1994) Tissue treatment for whole mount internal lectin staining in the nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus . Histochemistry 101, 379–384.CrossRefGoogle ScholarPubMed
Brooks, S.A., Leathem, A.J.C. & Schumacher, U. (1997) Lectin histochemistry: a concise practical handbook. Oxford, Bios Scientific Publishers.CrossRefGoogle Scholar
Buchmann, K. & Lindenstrom, T. (2002) Interactions between monogenean parasites and their fish hosts. International Journal for Parasitology 32, 309–319.CrossRefGoogle ScholarPubMed
Danguy, A. & Gabius, H.J. (1993) Lectins and neoglycoproteins – attractive molecules to study glycocconjugates and accessible sugar-binding sites of lower vertebrates histochemically. pp. 241–252 in Gabius, H.J. & Gabius, S. (Eds) Lectins and glycobiology. Berlin, Heidelberg, New York, Springer-Verlag.CrossRefGoogle Scholar
Ergens, R. & Lom, J. (1970) Causative agents of fish diseases. Prague, Publishing House Academia (in Czech).Google Scholar
Gelnar, M., Svobodová, Z. Vykusová, B. (1989) Eupdiplozoon nipponicum (Goto 1891)–a new parasite of carp in Czechoslovak ponds. Bulletin of Czech Fisheries 1, 5–12 (in Czech).Google Scholar
Gobert, G.N., Jones, M.K. & Stenzel, D.J. (1998) Ultrastructural analysis of the adult Schistosoma japonicum by lectin cytochemistry. International Journal for Parasitology 28, 1445–1452.CrossRefGoogle ScholarPubMed
Hokke, C.K. & Deelder, A.M. (2001) Schistosome glycoconjugates in host–parasite interplay. Glycoconjugate Journal 18, 537–587.CrossRefGoogle ScholarPubMed
Horák, P. (1995) Developmentally regulated expression of surface carbohydrate residues on larval stages of the avian schistosome Trichobilharzia szidati . Folia Parasitologica 42, 255–265.Google ScholarPubMed
Horejsi, V. (1977) Affinity methods in lectin study. PhD thesis, Charles University, Prague, Czech Republic (in Czech).Google Scholar
Joachim, A., Ruttkowski, B. & Daugschies, A. (1999) Changing surface antigen and carbohydrate patterns during the development of Oesophagostomum dentatum . Parasitology 119, 491–501.CrossRefGoogle ScholarPubMed
Khotenovsky, I.A. (1985) Fauna of the USSR. Monogenea. The subclass Octomacrinea Khotenovsky. 260 pp. Leningrad, Publishing House, Nauka (in Russian).Google Scholar
Köster, B. & Strand, M. (1994) Schistosoma mansoni : immunolocalization of two different fucose-containing carbohydrate epitopes. Parasitology 108, 433–446.CrossRefGoogle ScholarPubMed
Leducq, R. & Gabrion, C. (1992) Developmental changes of Echinococcus multilocularis metacestodes revealed by tegumental ultrastructure and lectin-binding sites. Parasitology 104, 129–141.CrossRefGoogle ScholarPubMed
Lis, H. & Sharon, N. (1986) Lectins as molecules and as tools. Annual Review of Biochemistry 55, 35–67.CrossRefGoogle ScholarPubMed
Pearse, A.G.E. (1960) Histochemistry, pure and applied. 759 pp. 2nd edn. London, J & A Churchill.Google Scholar
Nordmann, A. (1832) Mikrographische Beiträge zur Naturgeschichte der wirbellosen Tiere. Berlin, G. Reimer.Google Scholar
Romeis, B. (1989) Mikroskopische Technik. 697 pp. München, Wien, Baltimore, Aufl. Urban & Schwarzenberg.Google Scholar
Simpson, A.J.G., Rumjanek, F.D., Payeres, G. & Evans, W.H. (1981) Glycosyl transferase activities are associated with the surface membrane in adult Schistosoma mansoni . Molecular and Biochemical Parasitology 4, 107–115.CrossRefGoogle ScholarPubMed
Simpson, A.J.G., Correa-Oliveira, R., Smithers, S.R. & Sher, A. (1983) The exposed carbohydrates of schistosomula of Schistosoma mansoni and their modification during maturation in vivo. Molecular and Biochemical Parasitology 8, 191–205.CrossRefGoogle ScholarPubMed
Smyth, J.D. & Halton, D.W. (1983) The physiology of trematodes. 446 pp. 2nd edn. Cambridge, Cambridge University Press.Google Scholar
Sterba, G. (1957) Zur Morphologie und Biologie der Gattung Diplozoon . Zoologischer Anzeiger 158, 181–196.Google Scholar
Uchikawa, R. & Loker, E.S. (1991) Lectin-binding properties of the surface of in vitro-transformed Schistosoma mansoni and Echinostoma paraensei sporocysts. Journal of Parasitology 77, 724–748.CrossRefGoogle ScholarPubMed
Zanetta, J.P., Badache, A., Maschke, S., Marschal, P. & Kuchler, S. (1994) Carbohydrates and soluble lectins and the regulation of cell adhesion and proliferation. Histology and Histopathology 9, 385–412.Google ScholarPubMed
Zeller, E. (1872) Untersuchungen über die Entwicklung des Diplozoon paradoxum . Zeitschrift für Wissenschaftliche Zoologie 22, 168–180.Google Scholar