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Successful detection, expression and purification of the alternatively spliced truncated Sm14 antigen of an Egyptian strain of Schistosoma mansoni

Published online by Cambridge University Press:  24 July 2014

R.E. Ewaisha ,
M. Bahey-El-Din ,
S.F. Mossallam ,
A.M. Khalil  and
H.M. Aboushleib
R.E. Ewaisha
Affiliation:
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
M. Bahey-El-Din*
Affiliation:
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
S.F. Mossallam
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt
A.M. Khalil
Affiliation:
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
H.M. Aboushleib
Affiliation:
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
*
*Fax: +2 034873273 E-mail: [email protected]
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Abstract

Schistosoma mansoni causes intestinal schistosomiasis, a disease that is prevalent in several regions worldwide. To date, a protective vaccine against S. mansoni is still lacking. Several promising antigens have been discovered and evaluated for vaccine protection, such as Sm14 and Sm28GST. In this short communication, we report the successful detection of an alternatively spliced truncated form of Sm14 which was highly expressed in an Egyptian strain of S. mansoni. This truncated Sm14 (TrSm14) protein was formerly reported to be practically non-existent and its complementary DNA (cDNA) was thought to be ‘a rare misprocessing of mRNA precursor’. Our finding demonstrates that there is inter-strain variation in the S. mansoni transcriptome and subsequently in the role/function of the expressed proteins. We expressed TrSm14 successfully in Escherichia coli as a fusion protein with the schistosomal antigen Sm28GST. The fusion protein was purified using metal affinity chromatography and was found to be reactive with serum from S. mansoni-infected patients. This suggests a possible diagnostic value for this protein in detection of anti-schistosomal antibodies. In addition, this fusion protein could offer a potential bivalent vaccine candidate against S. mansoni that is worthy of further investigation.

Type
Short Communications
Information
Journal of Helminthology , Volume 89 , Issue 6 , November 2015 , pp. 764 - 768
Copyright
Copyright © Cambridge University Press 2014 

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