Hostname: page-component-745bb68f8f-l4dxg Total loading time: 0 Render date: 2025-01-09T03:16:50.680Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on the Serological Response in Sheep to Naturally Acquired Gastro-intestinal Nematodes. I. Preparation of Antigens and Evaluation of Serological Techniques

Published online by Cambridge University Press:  18 November 2009

E. J. L. Soulsby
E. J. L. Soulsby
Affiliation:
Department of Animal Pathology, University of Cambridge
Get access Rights & Permissions [Opens in a new window]

Extract

Antigens were prepared by low temperature methods to preserve “functional” antigen. These antigens showed marked cross reactivity and were satisfactory in complement fixation tests.

The passive haemagglutination test using “functional” antigen gave titres which were higher than those obtained with the complement fixation test. High antibody titres were observed in sheep naturally infested with nematodes and a relationship between antibody titre and egg count was demonstrated.

Type
Research Papers
Information
Journal of Helminthology , Volume 30 , Issue 2-3 , October 1956 , pp. 129 - 142
Copyright
Copyright © Cambridge University Press 1956

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Borduas, A. G. and Grabar, P., 1953.—“L'hémagglutination passive dans la recherche des anticorps antiproteiques.” Ann. Inst. Pasteur., 84, 903. (W.L. 857).Google Scholar
Boyden, S. V., 1951a.—“The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera.” J. Exp. Med., 93, 107. (W.L. 11189).Google Scholar
Boyden, S. V., 1951b.—“Bacterial haemagglutination and allied subjects.” Ph.D. Thesis. Cambridge.Google Scholar
Bukantz, S. C., Rein, C. R. and Kent, J. F., 1946—“Studies in Complement Fixation. II. Preservation of sheep's blood in citrate dextrose mixtures (modified Alsevers solution) for use in the complement fixation reaction.” J. Lab. Clin. Med., 31, 394. (W.L. 11285).Google Scholar
Coombs, R. R. A. and Fiset, M. L., 1954.—“Detection of complete and incomplete antibodies to egg albumen by means of a sheep red cell-egg albumen antigen unit.” Brit. J. exp. Path., 35, 472. (W.L. 3566).Google Scholar
Dunn, A. M., 1954.—“The preparation of helminth antigen from fresh Bunostomum trigonocephalum.” J. Helminth., 28, 9. (W.L. 11224C).CrossRefGoogle ScholarPubMed
Grabar, P., Boyden, S. V., Taquet, A. and Borduas, A. G., 1952.—“Mise en évidence de deux anticorps différents dans les sérums tuberculeux par hémagglutination passive.” C. R. Acad. Sci., 234, 899. (W.L. 6628).Google Scholar
Hawkins, P. A. and Cole, C. L., 1945.—“Studies on sheep parasites. V. Immunity to gastro-intestinal nematodes.” J. Parasit., 31, 113. (W.L. 11428).Google Scholar
Heidelberger, M. and Kendall, F. E., 1935.—“A quantitative theory of the precipitin reaction. III. The reaction between crystalline egg albumen and its homologous antibody.” J. exp. Med., 62, 697. (W.L. 11189).CrossRefGoogle Scholar
Mackie, T. J., and Finkelstein, M. H., 1928.—“A study of non-specific complement fixation with particular reference to the interaction of normal serum and certain non-antigenic substances.” J. Hyg., Camb., 28, 172. (W.L. 11233).Google Scholar
Mauss, E. A., 1940.—“The in vitro effect of immune serum upon Trichinella spiralis larvae.” Amer. J. Hyg., 32, D, 80. (W.L. 600a)Google Scholar
Oliver-Gonzalez, J., 1940.—“In vitro action of immune serum on larvae and adults of Trichinella spiralis.” J. infect. Dis., 67, 292. (W.L. 11250)Google Scholar
Oliver-Gonzalez, J., 1941.—“The dual antibody basis of acquired immunity in Trichinosis.” J. infect. Dis., 69, 254.Google Scholar
Oliver-Gonzalez, J., 1943.—“Antigenic analysis of the isolated tissue and body fluids of the roundworm Ascaris lumbricoides var. suum.” J. infect. Dis., 72, 202.Google Scholar
Oliver-Gonzalez, J., 1946.—“Functional antigens in helminths.” J. infect. Dis., 78, 232.Google Scholar
Oliver-Gonzalez, J., 1946.—“Immunological relationships among polysaccharides from various infectious organisms.” J. infect. Dis., 79, 221.Google Scholar
Otto, G. F., 1940.—“A serum antibody in dog actively immunized against the hookworm, A. caninum.” Amer. J. Hyg., 31, D, 23. (W.L. 600a)Google Scholar
Roth, H., 1945.—“Serodiagnosis of Trichinosis by microscopically testing with living Trichinae larvae.” Nature, Loud., 155, 785. (W.L. 14900)Google Scholar
Sarles, M. P., 1938.—“In vitro action of immune rat serum on the nematode Nippostrongylus muris.” J. infect. Dis., 62, 337. (W.L. 11250)Google Scholar
Sarles, M. P., and Talliaferro, W. H., 1936.—“The local points of defense and the passive transfer of acquired immunity to Nippostrongylus muris.” J. infect. Dis., 59, 207.CrossRefGoogle Scholar
Stewart, D. F., 1950a.—“Studies on resistance of sheep to infestation with Haemonchus contortus and Trichostrongylus spp. and on the immunological reactions of sheep exposed to infestation. I. The preparation of antigens for the complement fixation test and the reactivity of the biochemical fractions of H. contortus.” Australian J. Agric. Res., 1, 285. (W.L. 2244a)Google Scholar
Stewart, D. F., 1950b.—“II. The antibody response to infestation with H. contortus.” Australian J. Agric. Res., 1, 301.Google Scholar
Stewart, D. F., 1950c.—“III. The antibody response to infestation with Tricho strongylus spp.” Australian J. Agric. Res., 1, 413.Google Scholar
Stewart, D. F., 1950d.—“IV. The antibody response to natural infestation and the ‘self cure’ phenomenon.” Australian J. Agric. Res., 1, 427.Google Scholar
Wadsworth, A., Maltaner, E. and Maltaner, F., 1931.—“The quantitative determination of the fixation of complement by immune serum and antigen.” J. Immunol., 21, 313. (W.L. 11238)CrossRefGoogle Scholar
Wadsworth, A., Maltaner, E. and Maltaner, F., 1938.—“Quantitative studies of the reaction of complement fixation with tuberculous immune serume and antigen.” J. Immunol., 35, 93.Google Scholar