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Spermiogenesis in two paramphistomes from Nile fish in Egypt: an ultrastructural study

Published online by Cambridge University Press:  01 September 2007

A.A. Ashour ,
K. Garo  and
I.S. Gamil
A.A. Ashour
Affiliation:
Faculty of Science, Ain Shams University, Abbassia, 11566 Cairo, Egypt
K. Garo*
Affiliation:
Faculty of Science, Cairo University, Giza, Egypt
I.S. Gamil
Affiliation:
Faculty of Science, Helwan University, Egypt
*
*E-mail:[email protected]
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Abstract

The process of spermiogenesis in two paramphistomes, Sandonia sudanensis and Basidiodiscus ectorchis from the Nile fish Synodontis schall in Egypt was studied by transmission electron microscopy. Spermiogenesis is characterized by the outgrowth of the zone of differentiation, presenting two basal bodies separated by a microtubule organizing centre, each basal body developing into a flagellum. Proximodistal fusion of these flagella with a median cytoplasmic extension gives rise to the spermatozoon. The mature spermatozoon possesses two axonemes of the 9+‘1’ pattern typical of parasitic helminths. There are few ultrastructural studies on spermiogenesis in paramphistomes, which are considered the most primitive digenetic trematodes. The present study provides new and more detailed information on this process, including the presence of a lateral flange and external ornamentation of the cell membrane. The value of sperm ultrastructure as a taxonomic tool in phylogeny is also discussed.

Type
Research Papers
Information
Journal of Helminthology , Volume 81 , Issue 3 , September 2007 , pp. 219 - 226
Copyright
Copyright © Cambridge University Press 2007

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References

Ashour, A.A., Hassan, N.H.A., Ibrahim, A.M. & El-Kabbany, A.I. (1995) The karyotypes of Calicophoron microbothrium and Carmyerius gregarius from Egyptian ruminants. Journal of Helminthology 69, 107–111.CrossRefGoogle ScholarPubMed
Ashour, A.A., Stietieh, F.M. & Khalil, A.A. (2001) Studies on the liver fluke Fasciola in Egypt: spermatogenesis. Egyptian Journal of Medical Laboratory Science 10, 79–92.Google Scholar
Ashour, A.A., Soliman, M.I., Mezied, N.G. & Mohammed, A.H. (2003) Ultrastructural development of spermatozoa in Macrogyrodactylus congolensis (Prudhoe, 1957) Yamaguti, 1963 (Monogena: Monopisthocotylea, Gyrodactylidae). Egyptian Journal of Zoology 40, 99–110.Google Scholar
Ashour, A.A., Tohamy, A.A. & Gamil, I.S. (2006) Karyotype analysis of two paramphistomes infecting Nile fish. Cytologia 71, 35–39.CrossRefGoogle Scholar
Baptista-Farias, M.F.D., Kohn, A. & Cohen, S.C. (2001) Ultrastructure of spermatogenesis and sperm development in Saccocoelioides godoyi, Kohn & Frides, 1986 (Digenea, Haploporidae). Memórias do Instituto Oswaldo Cruz 96, 61–70.CrossRefGoogle ScholarPubMed
Gracenea, M., Ferrer, J.R. & Gonzalez-Moreno, O. (1997) Ultrastructural study of spermatogenesis and the spermatozoon in Postorchigenes gymnescicus (Trematoda: Lecithodendridae). Journal of Morphology 234, 223–232.3.0.CO;2-A>CrossRefGoogle Scholar
Grossman, A.I. & Cain, G.D. (1981) Karyotypes and chromosome morphologies of Megalodiscus temperatus and Philophthalmus gralli. Journal of Helminthology 55, 71–78.CrossRefGoogle Scholar
Gupta, B.C., Parshad, V.R. & Guraya, S.S. (1986) Morphological and histochemical studies on the development of testis and spermatogenesis in the ruminal trematode of sheep, Paramphistomum cervi (Digenea: Paramphistomatidae). Folia Parasitologica 33, 131–144.Google ScholarPubMed
Iomini, C. & Justine, J.L. (1997) Spermiogenesis and spermatozoon of Echinostoma caproni (Platyhelminthes, Digenea): transmission and scanning electron microscopy, and tubulin immuno-cytochemistry. Tissue and Cell 29, 107–118.CrossRefGoogle Scholar
Justine, J.L., Lambert, A. & Mattei, X. (1985) Spermatozoon ultrastructure and phylogenetic relationships in the monogeneans (Platyhelminthes). International Journal for Parasitology 15, 601–608.CrossRefGoogle ScholarPubMed
Justine, J.L., Jamieson, B.G.M. & Southgate, V.R. (1993) Homogeneity of sperm structure in six species of schistosome (Digenea, Platyhelminthes). Annales de Parasitologie Humaine et Comparée 68, 185–187.CrossRefGoogle Scholar
Kiger, L., Rashid, A.K., Griffon, N., Haque, M., Moens, L., Gibson, Q.H., Poyart, C. & Marden, M.C. (1998) Trematode hemoglobins show exceptionally high oxygen affinity. Biophysical Journal 75, 990–998.CrossRefGoogle ScholarPubMed
Levron, C., Ternengo, S. & Marchand, B. (2004) Ultrastructure of spermiogenesis and the spermatozoon of Monorchis parvus Looss, 1902 (Digenea, Monorchiidae), a parasite of Diplodus annularis (Pisces, Teleostei). Parasitology Research 93, 102–110.CrossRefGoogle ScholarPubMed
Li, M.M. & Wang, X.Y. (1997) Spermatogenesis and ultrastructure of the metaphase chromosomes in Ceylonocotyle scoliocoelium (Digenea: Paramphistomidae). Acta Zoologica Sinica 43, 1–9.Google Scholar
Ndiaye, P., Miquel, J., Ba, C., Feliu, C. & Marchand, B. (2002) Spermiogenesis and sperm ultrastructure of Scaphiostomum palaearctium Mas-Coma, Esteban & Valero, 1986 (Trematoda, Digenea, Brachylaimidae). Acta Parasitologica 47, 259–271.Google Scholar
Ndiaye, P., Miquel, J., Fons, R. & Marchand, B. (2003) Spermiogenesis and sperm ultrastructure of the liver fluke Fasciola hepatica L., 1758 (Digenea, Fasciolidae): transmission and scanning electron microscopy, and tubulin immuno-cytochemistry. Acta Parasitologica 48, 182–194.Google Scholar
Ndiaye, P., Miquel, J., Ba, C. & Marchand, B. (2004) Spermiogenesis and ultrastructure of the spermatozoon of the liver fluke Fasciola gigantica Cobbold, 1856 (Digenea: Fasciolidae), a parasite of cattle in Senegal. Journal of Parasitology 90, 30–40.CrossRefGoogle ScholarPubMed
Nollen, P.M. & Pyne, J.L. (1979) Observations on spermatogenesis and insemination behaviour of Megalodiscus temperatus adults in frogs. Journal of Parasitology 65, 35–37.CrossRefGoogle ScholarPubMed
Pamplona-Basilio, M.C., Baptista-Farias, M.F.D. & Cohn, A. (2001) Spermatogenesis and spermiogenesis in Didymocystis wedli Ariola, 1902 (Didymozoidae, Digenea). Memorias do Instituto Oswaldo Cruz 96, 1153–1159.CrossRefGoogle ScholarPubMed
Rohde, K. (1980) Some aspects of the ultrastructure of Gotocotyla secunda and Hexostoma thynni. Angewandte Parasitologie 21, 32–48.Google Scholar
Rohde, K., Watson, N. & Cannon, L.R.G. (1988) Comparative ultrastructural studies of the epidermis, sperm, and nerve fibres of Cleistogamia longicirrus and Seritia stichopi (Rhabdocoela, Umagillidae). Zoologica Scripta 17, 337–345.CrossRefGoogle Scholar
Taelab, A.A. (2000) Studies on some paramphistomes from Egypt. PhD thesis, Zagazig University, Egypt.Google Scholar
Watson, N.A. & Rohde, K. (1995) Re-examination of spermiogenesis of Multicotyle purvisi (Platyhelminthes, Aspidogastrea). International Journal for Parasitology 25, 579–586.CrossRefGoogle ScholarPubMed
White, M.J.D. (1973) Animal cytology and evolution. 454 pp. 2nd edn. Cambridge, Cambridge, University Press.Google Scholar