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Spermatological characteristics of Elstia stossichianum (Digenea, Mesometridae) from the intestine of the cow bream (Sarpa salpa) off Dakar, Senegal

Published online by Cambridge University Press:  16 October 2012

A.J.S. Bakhoum ,
P.I. Ndiaye ,
C.T. Bâ  and
J. Miquel
A.J.S. Bakhoum
Affiliation:
Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, s/n, E-08028Barcelona, Spain Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, E-08028Barcelona, Spain
P.I. Ndiaye
Affiliation:
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal
C.T. Bâ
Affiliation:
Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, Faculty of Sciences and Techniques, Cheikh Anta Diop University of Dakar, Senegal
J. Miquel*
Affiliation:
Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII, s/n, E-08028Barcelona, Spain Institut de Recerca de la Biodiversitat, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, E-08028Barcelona, Spain
*
*Fax: +34 93 402 45 04 E-mail: [email protected]
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Abstract

The current study was designed to increase the spermatological ultrastructural database on Digenea, thus contributing to the future establishment of phylogenetic relationships within this group based on ultrastructural characteristics of both spermiogenesis and spermatozoa. Spermiogenesis in Elstia stossichianum begins with the formation of a differentiation zone containing two centrioles, two striated rootlets, a nucleus, several mitochondria and an intercentriolar body constituted by seven electron-dense layers. Each centriole develops into a free flagellum growing orthogonally to the median cytoplasmic process. Proximo-distal fusion of the flagella with the median cytoplasmic process occurs after flagellar rotation. Both nucleus and mitochondria migrate before the complete proximo-distal fusion of both flagella with the median cytoplasmic process. Finally, the constriction of the ring of arched membranes gives rise to the young spermatozoon. The mature spermatozoon of E. stossichianum exhibits two axonemes of different length of the 9+‘1’ trepaxonematan pattern, a nucleus, a mitochondrion, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, a lateral expansion, spine-like bodies, cytoplasmic ornamented buttons and granules of glycogen. The formation of cytoplasmic ornamented buttons during the final stages of spermiogenesis is reported here for the first time.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 4 , December 2013 , pp. 422 - 431
Copyright
Copyright © Cambridge University Press 2012 

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