Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-25T06:12:47.093Z Has data issue: false hasContentIssue false
  • English
  • Français

Serological Investigations with Loa loa Antigens

Published online by Cambridge University Press:  05 June 2009

E. O. Ogunba
E. O. Ogunba
Affiliation:
Department of Medical Microbiology, University College Hospital, University of Ibadan, Nigeria
Get access Rights & Permissions [Opens in a new window]

Extract

Antigens prepared from adult worms and microfilariae of Loa loa were used for an assessment of the sensitivity and specificity in diagnosis of double immuno-diffusion in agar gel, haemagglutination and fluorescent antibody tests.

Both antigens formed a broad band which moved slowly towards the cathode on electrophoresis, and each antigen separated into two protein fractions when passed through Sephadex G-200.

Both precipitating and non-precipitating antibodies were detected in sera from Loa loa patients. The haemagglutination test and fluorescent antibody test were sensitive in detecting Loa loa antibodies and would be of diagnostic value, but the double diffusion test in agar-gel was insensitive and would have a limited value in loiasis diagnosis.

Type
Research Article
Information
Journal of Helminthology , Volume 46 , Issue 3 , September 1972 , pp. 241 - 250
Copyright
Copyright © Cambridge University Press 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Culbertson, J. T., Rose, H. M., AND Demarest, C. R., 1944.—“Loiasis and onchocerciasis; a new antigen for their diagnosis by skin test.” Am. J. Hyg., 39, 152–155.Google Scholar
Franks, M. B. and Stoll, N. R., 1945.—“The isolation of microfilariae from blood for use as antigen.” J. Parasit., 31, 158–162.CrossRefGoogle Scholar
Jacobs, L. and Lunde, M. N., 1957.—“Haemagglutination test for toxoplasmosis.” Science, 125, 1035.CrossRefGoogle ScholarPubMed
Jayewardene, L. G. and Wijayaratnam, Y., 1968.—“The fluorescent antibody test in the serological diagnosis of the causative organisms of tropical eosinophilia and filariasis.” J. Helminth., 42, 57–64.CrossRefGoogle ScholarPubMed
Oliver-Gonzalez, J. and Bercovitz, Z. T., 1944.—“Precipitin reactions with antigen prepared from microfilariae of Wuchereria bancrofti (preliminary report)”. Am. J. Trop. Med., 24, 315–316.CrossRefGoogle Scholar
Ouchterlony, O., 1953.—“Antigen-antibody reactions in gels: types of reactions in co-ordinated systems of diffusion.” Act path, microbiol. scandinav., 32,231–240.CrossRefGoogle Scholar
Sadun, E. H., 1963.—“Immunodiagnosis of helminth infections”. Amer. J. Hyg. Monographic Series, 22, pp. 122.Google Scholar
Sawyer, T. K. and Weinstein, P. P., 1963.—“Studies on microfilariae of the dog heartworm Dirofilaria immitis: separation of parasites from whole blood. J. Parasit., 49, 39–45.CrossRefGoogle ScholarPubMed
Tanaka, H., Kobayashi, J., Matsuda, H. and Sasa, M., 1968.—“Hemagglutination test with Litomosoides carinii antigen in the diagnosis of Cotton Rat filariasis. Jap. J. Exp. Med., 38, 19–25.Google ScholarPubMed