Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-24T23:42:07.188Z Has data issue: false hasContentIssue false
  • English
  • Français

Serodiagnostic potential of chemically synthesized glycosphingolipid antigens in an enzyme-linked immunosorbent assay for alveolar echinococcosis

Published online by Cambridge University Press:  12 April 2024

K. Yamano ,
N. Hada ,
T. Yamamura ,
T. Takeda ,
H. Honma  and
Y. Sawada
K. Yamano*
Affiliation:
Department of Biological Science, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060-0819, Japan
N. Hada
Affiliation:
Kyoritsu University of Pharmacy, Tokyo, Japan
T. Yamamura
Affiliation:
Kyoritsu University of Pharmacy, Tokyo, Japan
T. Takeda
Affiliation:
Kyoritsu University of Pharmacy, Tokyo, Japan
H. Honma
Affiliation:
Department of Biological Science, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060-0819, Japan
Y. Sawada
Affiliation:
Department of Biological Science, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060-0819, Japan
*
*Corresponding author: Fax: 81 11 736 9476, Email: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In the serodiagnosis of alveolar echinococcosis, the detection of specific reactions against not only protein but also carbohydrate antigen is useful and both antigens supplement each other. Though recombinant protein antigens have recently advanced, the preparation of carbohydrate antigen still depends on extraction from crude antigens. In the latter case, it is not conventional to obtain carbohydrate antigen as a single component for examination and research. Therefore, chemically synthesized carbohydrate antigens were prepared for serodiagnosis by the enzyme-linked immunosorbent assay (ELISA). Four antigens with the structure of glycosphingolipids from Echinococcus multilocularis were examined and one antigen, Galβ1-6(Fucα1-3)Galβ1-6Galβ1-ceramide, was found to show significant serodiagnostic potential in differentiating alveolar from cystic echinococcosis.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 4 , December 2006 , pp. 387 - 391
Copyright
Copyright © Cambridge University Press 2006

References

Felleisen, R. & Gottstein, B. (1994) Comparative analysis of full-length antigen II/3 from Echinococcus multilocularis and E. granulosus. Parasitology 109, 223–232.CrossRefGoogle ScholarPubMed
Frosch, P.M., Frosch, M., Pfister, T., Schaad, V. & Bitter-Suermann, D. (1991) Cloning and characterization of an immunodominant major surface antigen of Echinococcus multilocularis. Molecular and Biochemical Parasitology 48, 121–130.CrossRefGoogle ScholarPubMed
Furuya, K., Sato, H. & Kumagai, M. (1987) Application of Western blotting method for immunoserologic diagnosis of hydatidosis. Journal of Clinical and Experimental Medicine (IGAKU NO AYUMI) 141, 41–42 (in Japanese).Google Scholar
Furuya, K., Sasaki, S., Honma, H., Kumagai, M., Sato, N., Takahashi, M. & Uchino, J. (1989) Serologic investigations of human alveolar hydatid disease by Western blotting and indirect histo-immunoperoxidase techniques. Japanese Journal of Parasitology 38, 184–193.Google Scholar
Furuya, K., Nishizuka, M., Honma, H., Kumagai, M., Sato, N., Takahashi, M. & Uchino, J. (1990) Prevalence of human alveolar echinococcosis in Hokkaido as evaluated by Western blotting. Japanese Journal of Medical Science and Biology 43, 43–49.CrossRefGoogle ScholarPubMed
Furuya, K., Kawanaka, M., Yamano, K., Sato, N. & Honma, H. (2004) Laboratory evaluation of commercial immunoblot assay kit for serodiagnosis of Echinococcus infections using sera from patients with alveolar hydatidosis in Hokkaido. Journal of the Japanese Association for Infectious Diseases 78, 320–326.Google ScholarPubMed
Hada, N., Hayashi, E. & Takeda, T. (1999) Synthetic studies on glycosphingolipids from the parasite Echinococcus multilocularis. Carbohydrate Research 316, 58–70.CrossRefGoogle ScholarPubMed
Helbig, M., Frosch, P., Kern, P. & Frosch, M. (1993) Serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens. Journal of Clinical Microbiology 31, 3211–3215.CrossRefGoogle ScholarPubMed
Hubert, K., Cordero, E., Frosch, M. & Solomon, F. (1999) Activities of the EM10 protein from Echinococcus multilocularis in cultured mammalian cells demonstrate functional relationships to ERM family members. Cell Motility and the Cytoskeleton 42, 178–188.3.0.CO;2-3>CrossRefGoogle ScholarPubMed
Hülsmeier, A.J., Gehrig, P.M., Geyer, R., Sack, R., Gottstein, B., Deplazes, P. & Köler, P. (2002) Major Echinococcus multilocularis antigen is a mucin-type glycoprotein. Journal of Biological Chemistry 277, 5742–5748.CrossRefGoogle ScholarPubMed
Ito, A., Xiao, N., Liance, M., Sato, M.O., Sako, Y., Mamuti, W., Ishikawa, Y., Nakao, M., Yamasaki, H., Nakaya, K., Bardonnet, K., Bresson-Hadni, S. & Vuitton, D.A. (2002) Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test. Journal of Clinical Microbiology 40, 4161–4165.CrossRefGoogle Scholar
Kannagi, R., Nudelman, E., Levery, S.B. & Hakomori, S. (1982) A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to developmentally-regulated antigen, SSEA-1. Journal of Biological Chemistry 257, 14865–14874.CrossRefGoogle Scholar
Kimura, H., Furuya, K., Kawase, S., Sato, C., Yamano, K., Takahashi, K., Uraguchi, K., Ito, T., Yagi, K. & Sato, N. (1999) Recent epidemiologic trends in alveolar echinococcosis prevalence in humans and animals in Hokkaido. Journal of the Japanese Association for Infectious Diseases 52, 117–120.CrossRefGoogle ScholarPubMed
Kouguchi, H., Suzuki, T., Yamano, K., Honma, H. & Sawada, Y. (2005) Characterization of various recombinant antigens from Echinococcus multilocularis for use in the immunodiagnosis. Protein Journal 24, 57–64.CrossRefGoogle ScholarPubMed
Marcus, D.M., Kundu, S.K. & Suzuki, A. (1981) The P blood group system: recent progress in immunochemistry and genetics. Seminars in Hematology 18, 63–71.Google Scholar
Nagano, M., Sato, C. & Furuya, K. (1995) Human alveolar echinococcosis seroprevalence assessed by Western blotting in Hokkaido. Japanese Journal of Medical Science and Biology 48, 157–161.CrossRefGoogle ScholarPubMed
Persat, F., Vincent, C., Mojon, M. & Petavy, A.F. (1991) Detection of antibodies against glycolipids of Echinococcus multilocularis metacestodes in sera of patients with alveolar hydatid disease. Parasite Immunology 13, 379–389.CrossRefGoogle ScholarPubMed
Persat, F., Bouhours, J.F., Mojon, M. & Petavy, A.F. (1992) Glycosphingolipids with Galβ1-6Gal sequences in metacestodes of the parasite Echinococcus multilocularis. Journal of Biological Chemistry 267, 8764–8769.CrossRefGoogle Scholar
Sato, C. & Furuya, K. (1994) Isolation and characterization of a diagnostic polysaccharide antigen from larval Echinococcus multilocularis. Japanese Journal of Medical Science and Biology 47, 65–71.CrossRefGoogle ScholarPubMed
Sato, H., Mitamura, H., Arai, J. & Kumagai, M. (1983a) Serological diagnosis of human hydatid diseases by enzyme-linked immunosorbent assay (Part 1) enzyme-linked immunosorbent assay by multilocular Echinococcus antigen. Report of the Hokkaido Institute of Public Health 33, 8–15 (in Japanese).Google Scholar
Sato, H., Mitamura, H., Arai, J. & Kumagai, M. (1983b) Serological diagnosis of human hydatid diseases by enzyme-linked immunosorbent assay (Part 2) application and evaluation on the health examination. Report of the Hokkaido Institute of Public Health 33, 16–20 (in Japanese).Google Scholar
Vogel, M., Gottstein, B., Muller, N. & Seeback, T. (1988) Production of a recombinant antigen of Echinococcus multilocularis with high immunodiagnostic sensitivity and specificity. Molecular and Biochemical Parasitology 31, 117–125.CrossRefGoogle ScholarPubMed
Watkins, W.M. (1980) Biochemistry and genetics of the ABO, Lewis and P blood group systems. Advances in Human Genetics 10, 1–136.Google ScholarPubMed
Yamamura, T., Hada, N., Kaburaki, A., Yamano, K. & Takeda, T. (2004) Synthetic studies on glycosphingolipids from Protostomia phyla: total syntheses of glycosphingolipids from the parasite, Echinococcus multilocularis. Carbohydrate Research 339, 2479–2759.CrossRefGoogle ScholarPubMed
Yamano, K., Yagi, K., Furuya, K., Sawada, Y. & Honma, H. (2005) Active alveolar hydatidosis with sero-negativity for antibody to the 18 kDa antigen. Journal of the Japanese Association for Infectious Diseases 58, 122–124.CrossRefGoogle Scholar