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Sequence variability in three mitochondrial DNA regions of Spirometra erinaceieuropaei spargana of human and animal health significance

Published online by Cambridge University Press:  20 July 2011

W. Liu ,
G.H. Liu ,
F. Li ,
D.S. He ,
T. Wang ,
X.F. Sheng ,
D.L. Zeng ,
F.F. Yang  and
Y. Liu
W. Liu
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China Hunan Biological and Electromechanical Polytechnic College, Changsha, Hunan Province410128, PR China
G.H. Liu
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China
F. Li
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China Hunan Biological and Electromechanical Polytechnic College, Changsha, Hunan Province410128, PR China
D.S. He
Affiliation:
Hunan Biological and Electromechanical Polytechnic College, Changsha, Hunan Province410128, PR China
T. Wang
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China
X.F. Sheng
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China
D.L. Zeng
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China
F.F. Yang
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China
Y. Liu*
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China
*
*Fax: +86 731 84673618 E-mail: [email protected]
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Abstract

Sequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 3 (cox3), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4) in Spirometra erinaceieuropaei spargana from different geographical regions in China was examined. A portion of each of the cox3 (pcox3), nad1 (pnad1) and nad4 genes (pnad4) were amplified separately from individual S. erinaceieuropaei spargana by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. The sequences of pcox3, pnad1 and pnad4 were 541, 607 and 847 bp in length, respectively. The A+T contents of the sequences were 68.39–68.76% (pcox3), 63.76–64.91% (pnad1) and 67.18–67.77% (pnad4), respectively, while the intra-specific sequence variations within each of the S. erinaceieuropaei spargana were 0–1.5% for pcox3, 0–2.8% for pnad1 and 0–2.7% for pnad4. Phylogenetic analysis using neighbour joining (NJ), maximum likelihood (ML) and maximum parsimony (MP) methods, indicated that all the spargana isolates in Hunan Province represented S. erinaceieuropaei. These findings demonstrated clearly the usefulness of the three mtDNA sequences for population genetics studies of S. erinaceieuropaei spargana of human and animal health significance.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 3 , September 2012 , pp. 271 - 275
Copyright
Copyright © Cambridge University Press 2011

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References

Chilton, N.B., Gasser, R.B. & Beveridge, I. (1995) Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). International Journal for Parasitology 25, 647–651.CrossRefGoogle Scholar
Cui, J., Lin, X.M., Zhang, H.W., Xu, B.L. & Wang, Z.Q. (2011) Sparganosis, Henan Province, central China. Emerging Infectious Diseases 17, 146–147.CrossRefGoogle ScholarPubMed
Felsenstein, J. (1995) PHYLIP (Phylogeny Inference Package), version 3.57c. Department of Genetics, University of Washington, Seattle.Google Scholar
Gasser, R.B., Zhu, X. & McManus, D.P. (1999) NADH dehydrogenase subunit 1 and cytochrome c oxidase subunit I sequences compared for members of the genus Taenia (Cestoda). International Journal for Parasitology 29, 1965–1970.CrossRefGoogle ScholarPubMed
Li, M.W., Lin, R.Q., Song, H.Q., Sani, R.A., Wu, X.Y. & Zhu, X.Q. (2008) Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance. Electrophoresis 29, 2912–2917.CrossRefGoogle ScholarPubMed
Li, M.W., Lin, H.Y., Xie, W.T., Gao, M.J., Huang, Z.W., Wu, J.P., Li, C., Lin, R.Q. & Zhu, X.Q. (2009) Enzootic sparganosis in Guangdong, China. Emerging Infectious Diseases 15, 1317–1318.CrossRefGoogle Scholar
Liu, G.H., Lin, R.Q., Li, M.W., Liu, W., Liu, Y., Yuan, Z.G., Song, H.Q., Zhao, G.H., Zhang, K.X. & Zhu, X.Q. (2011) The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans. Molecular Biological Report 38, 2249–2256.CrossRefGoogle Scholar
Liu, W., Zhao, G.H., Tan, M.Y., Zeng, D.L., Wang, K.Z., Yuan, Z.G., Lin, R.Q., Zhu, X.Q. & Liu, Y. (2010) Survey of Spirometra erinaceieuropaei spargana infection in the frog Rana nigromaculata of the Hunan Province of China. Veterinary Parasitology 173, 152–156.CrossRefGoogle ScholarPubMed
Ooi, H.K., Chang, S.L., Huang, C.C., Kawakami, Y. & Uchida, A. (2000) Survey of Spirometra erinaceieuropaei in frogs in Taiwan and its experimental infection in cats. Journal of Helminthology 74, 173–176.CrossRefGoogle ScholarPubMed
Page, R.D. (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357–358.Google ScholarPubMed
Pampiglione, S., Fioravanti, M.L. & Rivasi, F. (2003) Human sparganosis in Italy. Case report and review of the European cases. APMIS: Acta Pathologica, Microbiologica et Immunologica 111, 349–354.CrossRefGoogle ScholarPubMed
Strimmer, K. & Haeseler, A.V. (1996) Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Molecular Biology and Evolution 13, 964–969.CrossRefGoogle Scholar
Sun, H.Y., Zhu, X.Q., Xie, M.Q., Wu, X.Y., Li, A.X., Lin, R.Q. & Song, H.Q. (2006) Characterization of Cryptocaryon irritans isolates from marine fishes in Mainland China by ITS ribosomal DNA sequences. Parasitology Research 99, 160–166.CrossRefGoogle ScholarPubMed
Sun, H.Y., Noe, J., Barber, J., Coyne, R.S., Cassidy-Hanley, D., Clark, T.G., Findly, R.C. & Dickerson, H.W. (2009) Endosymbiotic bacteria in the parasitic ciliate Ichthyophthirius multifiliis. Applied and Environmental Microbiology 75, 7445–7452.CrossRefGoogle ScholarPubMed
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.CrossRefGoogle ScholarPubMed
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgin, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.CrossRefGoogle Scholar
Wiwanitkit, V. (2005) A review of human sparganosis in Thailand. International Journal of Infectious Diseases 9, 312–316.CrossRefGoogle ScholarPubMed
Zarowiecki, M.Z., Huyse, T. & Littlewood, D.T. (2007) Making the most of mitochondrial genomes – markers for phylogeny, molecular ecology and barcodes in Schistosoma (Platyhelminthes: Digenea). International Journal for Parasitology 37, 1401–1418.CrossRefGoogle ScholarPubMed
Zhao, G.H., Mo, X.H., Zou, F.C., Weng, Y.B., Lin, R.Q., Xia, C.M. & Zhu, X.Q. (2009) Genetic variability among Schistosoma japonicum isolates from different endemic regions in China revealed by sequences of three mitochondrial DNA genes. Veterinary Parasitology 162, 67–74.CrossRefGoogle Scholar