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Sequence analyses of mitochondrial gene may support the existence of cryptic species within Ascaridia galli

Published online by Cambridge University Press:  01 June 2022

Y. Zhao ,
S.-F. Lu  and
J. Li Open the ORCID record for J. Li [Opens in a new window]
Y. Zhao
Affiliation:
College of Animal Science and Technology, Xinyang Agriculture and Forestry University, Xinyang 464000, Henan, China College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China Jiangsu Key Laboratory of Zoonosis, Yangzhou 225009, Jiangsu, China
S.-F. Lu
Affiliation:
College of Animal Science and Technology, Xinyang Agriculture and Forestry University, Xinyang 464000, Henan, China
J. Li*
Affiliation:
College of Animal Science and Technology, Xinyang Agriculture and Forestry University, Xinyang 464000, Henan, China
*
Author for correspondence: J. Li, E-mail: [email protected]
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Abstract

Ascaridia galli (Nematoda: Ascaridiidae) is the most common intestinal roundworm of chickens and other birds with a worldwide distribution. Although A. galli has been extensively studied, knowledge of the genetic variation of this parasite in detail is still insufficient. The present study examined genetic variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene among A. galli isolates (n = 26) from domestic chickens in Hunan Province, China. A portion of the cox1 (pcox1) gene was amplified by polymerase chain reaction separately from adult A. galli individuals and the amplicons were subjected to sequencing from both directions. The length of the sequences of pcox1 is 441 bp. Although the intra-specific sequence variation within A. galli is 0–7.7%, the inter-specific sequence differences among other members of the infraorder Ascaridomorpha were 11.4–18.9%. Phylogenetic analyses based on the maximum likelihood method using the sequences of pcox1 confirmed that all of the Ascaridia isolates were A. galli, and also resolved three distinct clades. Taken together, the findings suggest that A. galli may represent a complex of cryptic species. Our results provide an additional genetic marker for the management of A. galli in chickens and other birds.

Keywords

Ascaridia galligenetic diversitymitochondrial DNAcryptic species
Type
Short Communication
Information
Journal of Helminthology , Volume 96 , 2022 , e39
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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