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Schistosoma haematobium in the baboon (Papio anubis): assessment of protection levels against either a single mass challenge or repeated trickle challenges after vaccination with irradiated schistosomula

Published online by Cambridge University Press:  05 June 2009

G.D.F. Reid ,
R.F. Sturrock ,
R.A. Harrison  and
R.P. Tarara
G.D.F. Reid
Affiliation:
Department of Parasitology, Institute of Primate Research, National Museums of Kenya, PO Box 24481, Nairobi, Kenya
R.F. Sturrock*
Affiliation:
Department of Medical Parasitology, London School Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, England
R.A. Harrison
Affiliation:
Department of Medical Parasitology, London School Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, England
R.P. Tarara
Affiliation:
Department of Parasitology, Institute of Primate Research, National Museums of Kenya, PO Box 24481, Nairobi, Kenya
*
†Author for correspondence.
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Abstract

Baboons vaccinated intramuscularly with three times 9000 20 krad irradiated Schistosoma haematobium schistosomula at monthly intervals were exposed percutaneously to either a single mass challenge of 3000 (VMC) or ten, weekly trickle challenges of 300 (VTC) normal S. haematobium cercariae. Unvaccinated mass (MCC) or trickle (TCC) challenge controls were exposed simultaneously. Faecal and urine egg production was delayed in the vaccinated groups which also had reduced adult, particularly female, worm recoveries. Total faecal, urine and tissue eggs were lower in the vaccinated groups, as also were the size of granulomata and the gross pathology and severity of inflammatory responses in the bladder and ureters, except for an increased proportion of tissue eggs in the livers of vaccinated animals. Differences in pathology between groups were less marked in the other organs. Most indices were reduced in the trickle versus the mass challenge control groups. Some of these trends were statistically significant, mostly in the trickle vaccination group (VTC), but others were not. Compared with the appropriate unvaccinated controls, the percentage reduction for the trickle challenge (VTC) group (73%) was three times greater than that of the mass challenge (VMC) group (23%). Overall, the protective effect of vaccination was more clearly demonstrated in the trickle than in the mass challenge groups. This conclusion is based on a single experiment. Nevertheless, because trickle infections probably approximate more closely to what humans receive naturally, it is recommended that they should be used for future testing of all potential vaccines in baboons.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 2 , June 1995 , pp. 139 - 147
Copyright
Copyright © Cambridge University Press 1995

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