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Schistosoma bovis, S. intercalatumandS. haematobium:methods forin vivoradiolabelling of cercariae with radioselenium

Published online by Cambridge University Press:  05 June 2009

Niels Ørnbjerg Christensen
Niels Ørnbjerg Christensen
Affiliation:
Danish Bilharziasis Laboratory, Jaegersborg Alle 1 D, DK-2920 Charlottenlund, Denmark
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Abstract

Cercariae of Schistosoma bovis, S. intercalatum and S. haematobium were radiolabelled by individual exposure of their respective host snails to 75Se-methionine in 1 ml water for a period of 20 hours. Maximum cercaria-bound radioactivity was obtained four to seven days after labelling of snails, but detectable levels of radioactivity incorporation were obtained with all three species for a period of 32 days. The radiolabelling procedure did not interfere with the production of cercariae or with the biological characteristics of the labelled larvae. The described research opens the way for the application of various radioisotope tracer systems in studies on host-parasite relationships between cercariae and schistosomula of terminal-spined species of schistosomes and the final host.

Type
Research Article
Information
Journal of Helminthology , Volume 55 , Issue 1 , March 1981 , pp. 9 - 12
Copyright
Copyright © Cambridge University Press 1981

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