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Scanning electron microscopical studies on the tegument of adult worms of Schistosoma mansoni originating from ultraviolet-irradiated and non-irradiated cercariae

Published online by Cambridge University Press:  11 April 2024

S.H. Mohamed*
Affiliation:
Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt
*
*Fax: 284 2123
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Abstract

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The surface topography and ultrastructural changes of Schistosoma mansoni worms developed from ultraviolet-irradiated cercariae were compared with those from non-irradiated cercariae. The tegument of worms developed from irradiated cercariae showed a variety of changes including the occurrence of oedemata and most of the tubercles being torn at the tip and having lost their spines on the dorsal surface of some male worms. In females, parts of the tegument were devoid of spines and surface lesions were present. The range and extent of these changes differed not only among individual worms but also between different regions of individual worms resulting in generalized deformities in those worms originating from irradiated cercariae.

Type
Review Article
Copyright
Copyright © Cambridge University Press 1999

References

Ariyo, A.A. & Oyerinde, J.P.O. (1990) Effect of ultraviolet radiation on survival, infectivity and maturation of Schistosoma mansoni cercariae. International Journal for Parasitology 20, 893897.CrossRefGoogle ScholarPubMed
Awad, A.H. & Probert, A.J. (1991) The effect of praziquantel on the ultrastructure of Schistosoma margrebowiei . Journal of Helminthology 5, 7988.CrossRefGoogle Scholar
Becker, B., Mehlhorn, H., Andrews, P., Thomas, H. & Eckert, J. (1980) Light and electron microscopic studies on the effect of praziquantel on Schistosoma mansoni, Dicrocoelium dendriticum and Fasciola hepatica (Trematoda) in vitro . Zeitschrift für Parasitenkunde 63, 113128.CrossRefGoogle ScholarPubMed
Claus, W.D. (1958) Radiation biology and medicine. Reading, Massachusetts, USA, Addison-Wesley Publishing Company, Inc.Google Scholar
Dean, D.A. (1983) Schistosoma and related genera: aquired resistance in mice. Experimental Parasitology 55, 1104.CrossRefGoogle Scholar
Dean, D.A., Murrell, K.D., Shoutai, X. & Mangold, B.M. (1983) Immunization of mice with ultraviolet-irradiated Schistosoma mansoni cercariae: a re-evaluation. American Journal of Tropical Medicine and Hygiene 32, 790793.CrossRefGoogle ScholarPubMed
Ellis, C. & Wells, A.A. (1941) The chemical action of the ultraviolet rays. New York, Reinhold Publishing Co.Google Scholar
Fallon, P.G., Fookes, R.E. & Warton, G.A. (1996) Temporal differences in praziquantel and oxamniquine induced tegumental damage to adult Schistosoma mansoni: implications for drug antibody synergy. Parasitology 112, 4758.CrossRefGoogle ScholarPubMed
Ghandour, A.M. & Webbe, G. (1975) The effect of ultra-violet radiation on cercariae of Schistosoma mansoni and Schistosoma haematobium. Journal of Helminthology 49, 153159.CrossRefGoogle ScholarPubMed
Harrop, R. & Wilson, R.A. (1993) Irradiation of Schistosoma mansoni cercariae impairs neuromuscular function in developing schistosomula. Journal of Parasitology 79, 286289.CrossRefGoogle ScholarPubMed
Irie, Y., Utsunomiya, H., Tanaka, M., Ohmae, H., Nara, T. & Yasuraoka, K. (1989) Schistosoma japonicum and S. mansoni ultrastructural damage in the tegument and reproductive organs after treatment with levo- and dextro-praziquantel. American Journal of Tropical Medicine and Hygiene 41, 204211.CrossRefGoogle ScholarPubMed
Latarject, R. & Gray, L.H. (1954) Definition of the terms “protection” and “restoration”. Acta Radiologica 41, 61.CrossRefGoogle Scholar
Lichtenberg, F.V. & Sadun, E.H. (1963) Parasite migration and host reaction in mice exposed to irradiated cercariae of Schistosoma mansoni . Experimental Parasitology 13, 256265.CrossRefGoogle Scholar
Miller, F.H., Tulloch, G.S. & Kuntz, R.E. (1972) Scanning electron microscopy of integumental surface of Schistosoma mansoni . Journal of Parasitology 58, 693698.CrossRefGoogle ScholarPubMed
Moloney, N.A., Bickle, Q.D. & Webbe, G. (1985) The induction of specific immunity against Schistosoma japonicum by exposure of mice to ultraviolet-attenuated cercariae. Parasitology 90, 313323.CrossRefGoogle ScholarPubMed
Moloney, N.A., Webbe, G. & Hinchcliffe, P. (1987) The induction of species-specific immunity against Schistosoma japonicum by exposure of rats to ultraviolet-attenuated cercariae. Parasitology 94, 4954.CrossRefGoogle Scholar
Moore, A.G. (1977) Ultrastructural changes due to treatment in vivo of Schistosoma mansoni with hycanthone. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 115.Google Scholar
Mostafa, O.M. (1995) Parasitological and biochemical studies on albino mice infected by Schistosoma mansoni cercariae irradiated by ultraviolet rays. MSc thesis, Ain Shams University.Google Scholar
Murrell, K.D., Dean, D.A. & Stanfford, E.E. (1975) Resistance to infection with Schistosoma mansoni after immunization with worm extracts or live cercariae: role of cytotoxic antibody in mice and guinea pigs. American Journal of Tropical Medicine and Hygiene 24, 955962.CrossRefGoogle ScholarPubMed
Olivier, L. & Stirewalt, M.A. (1952) An efficient method for exposure of mice to cercariae of Schistosoma mansoni . Journal of Parasitology 38, 1923.CrossRefGoogle ScholarPubMed
Perlowagora-Szumlewicz, A. (1964) Studies on acquired resistance to Schistosoma mansoni in mice exposed to X-irradiated cercariae. Bulletin of the World Health Organization 30, 401412.Google ScholarPubMed
Sabry, S.A. (1983) Effect of some antischistosomal drugs on the Egyptian strain of Schistosoma haematobium. PhD thesis, Ain Shams University.Google Scholar
Shalaby, I.M. (1994) Ultrastructural changes on the tegumental surface of Schistosoma mansoni (Egyptian strain) after in vitro treatment with praziquantel. Journal of the Egyptian German Society of Zoology 14, 397411.Google Scholar
Shalaby, I.M., Banaja, A.A. & Ghandour, A.M. (1991) Scanning electron microscopy of the tegumental surface of in vivo treated Schistosoma mansoni (Saudi Arabian geographical strain) with oxamniquine and praziquantel. Journal of the Egyptian Society of Parasitology 21, 797810.Google ScholarPubMed
Shi, Y.E., Jiang, C.F., Han, J.J., Li, Y.L. & Ruppel, A. (1993) Immunization of pigs against infection with Schistosoma japonicum using ultraviolet attenuated cercariae. Parasitology 106, 459462.CrossRefGoogle ScholarPubMed
Silk, M.H., Spence, I.M. & Gear, J.H.S. (1969) Ultrastructural studies of the blood fluke Schistosoma mansoni. I. The integument. South African Journal of Medical Science 34, 110.Google ScholarPubMed
Silk, M.H., Spence, I.M. & Buch, B. (1970) Observations of Schistosoma mansoni in the scanning electron microscope. South African Journal of Medical Science 35, 2329.Google ScholarPubMed
Smithers, S.R. & Terry, R.J. (1965) Acquired resistance to experimental infection of Schistosoma mansoni in the albino rat. Parasitology 55, 711717.CrossRefGoogle ScholarPubMed
Standen, O.D. & Fuller, K.A. (1959) Ultra-violet irradiation of the cercariae of Schistosoma mansoni: inhibition of development to adult stage. Transactions of the Royal Society of Tropical Medicine and Hygiene 53, 372379.CrossRefGoogle ScholarPubMed
Urbach, F. (1969) The biologic effects of ultraviolet radiation (with emphasis on the skin). London, Pergamon Press Ltd.Google Scholar
Wales, A. & Kusel, J.R. (1992) Biochemistry of irradiated parasite vaccines: suggested models for their mode of action. Parasitology Today 8, 358363.CrossRefGoogle ScholarPubMed