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Ribosomal DNA analysis of isolates of the liver fluke Opisthorchis pedicellata (Verma, 1927) from two siluroid fish species in India

Published online by Cambridge University Press:  22 June 2016

K. Choudhary  and
N. Agrawal
K. Choudhary
Affiliation:
Department of Zoology, University of Lucknow, Lucknow, 226007, UP, India
N. Agrawal*
Affiliation:
Department of Zoology, University of Lucknow, Lucknow, 226007, UP, India
*
*E-mail: [email protected]
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Abstract

The aim of this paper was to evaluate the genetic relationship between two isolates of Opisthorchis (O. pedicellata sp. 1, O. pedicellata sp. 2) from two siluroid fish hosts, based on three nuclear DNA (18S, internal transcribed spacer 1 (ITS1) and ITS2). Molecular analysis revealed that both isolates of 18S sequences showed low intraspecific variability (1.6%), while this was 1.9% for ITS1 and 2.6% for ITS2. The secondary structure of the ITS2 region exhibited a remarkable four-helix model, with helices 1 and 4 being relatively short, three U–U mismatches in helix 2 and the longest helix (3) having two UGGG motifs. The phylogenetic analyses, using neighbour–joining (NJ) and maximum parsimony (MP) methods of MEGA 6, demonstrate that both isolates form a sister clade with significant support (100%). Therefore, we concluded that both the isolates of O. pedicellata are of the same species but some variations may be due to the sympatric environment of variant hosts.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 3 , May 2017 , pp. 302 - 311
Copyright
Copyright © Cambridge University Press 2016 

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