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Restriction fragment length polymorphisms of Anisakinae larvae

Published online by Cambridge University Press:  05 June 2009

Kazuo Sugane ,
Liu Qing  and
Tadashi Matsuura
Kazuo Sugane
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
Liu Qing
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
Tadashi Matsuura
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
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Abstract

The analysis of restriction fragment length polymorphisms (RFLPs) was applied to distinguish several kinds of Anisakinae larvae, Anisakis larvae (type I) collected from two different paratenic hosts, Anisakis larvae (type II) and Contracaecum larvae. The patterns of the two different paratenic host-derived DNA of Anisakis larva (I) were exactly the same in hybridized fragments generated by six endonucleases. The quite different patterns in RFLPs of genomic DNA were observed among the Anisakis larva (I), Anisakis larva (II) and Contracaecum larvae. The results suggest that the RFLPs analysis may be useful for distinguishing Anisakinae larvae and clarifying the relationships between Anisakis larvae and their adult worms.

Keywords

AnisakislarvaeRFLPsContracaecumchemotaxonomy
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 4 , December 1989 , pp. 269 - 274
Copyright
Copyright © Cambridge University Press 1989

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