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Relative expression of antigen B coding gene of bubaline isolates of Echinococcus granulosus in fertile and sterile cysts

Published online by Cambridge University Press:  28 September 2009

D. Pan ,
A.K. Bera ,
S. Bandyopadhyay ,
S.K. Das ,
S. Bandyopadhyay ,
S. Bhattacharyya ,
B. Manna ,
S. De  and
D. Bhattacharya
D. Pan
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
A.K. Bera
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
S. Bandyopadhyay
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
S.K. Das
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
S. Bandyopadhyay
Affiliation:
NRC on Yak, Dirang790 101, Arunachal Pradesh, India
S. Bhattacharyya
Affiliation:
Department of Genetics, Bidhan Chandra Krishi Viswavidyalaya, Nadia741 252, West Bengal, India
B. Manna
Affiliation:
Department of Zoology, Ballygunge Science College, University of Calcutta, Kolkata700 019, West Bengal, India
S. De
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
D. Bhattacharya*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata700 037, West Bengal, India
*
*E-mail: [email protected]
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Abstract

This article communicates the relative quantification of five isoforms of antigen B (AgB) of Echinococcus granulosus. Relative expression of the AgB was quantified in active and inactive cysts. Cysts with germinal membrane, clear cyst fluid and protoscoleces showed uniform expression of the five isoforms and were utilized as control. Relative expression of AgB1 was the highest in cysts, where calcification has initiated. AgB2 and AgB4 were expressed more in fertile cysts irrespective of the condition of germinal membrane. The lowest expression of AgB3 was seen in calcified cysts. The relative expression of AgB5 could not be correlated with respect to the condition of the cyst because AgB5 is typically expressed by the adult stage of the parasite.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 3 , September 2010 , pp. 241 - 244
Copyright
Copyright © Cambridge University Press 2010

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