Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-23T03:15:36.511Z Has data issue: false hasContentIssue false
  • English
  • Français

Redescription and synonymization of Oscheius citri Tabassum, Shahina, Nasira and Erum, 2016 (Rhabditida, Rhabditidae) from India and its taxonomical consequences

Published online by Cambridge University Press:  21 April 2021

A. Rana ,
A.H. Bhat Open the ORCID record for A.H. Bhat [Opens in a new window] ,
A.K. Chaubey ,
V. Půža  and
J. Abolafia
A. Rana
Affiliation:
Nematology Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut250004, UP, India
A.H. Bhat*
Affiliation:
Nematology Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut250004, UP, India Department of Zoology, Government Degree College, Billawar 184204, Kathua, Jammu and Kashmir, India
A.K. Chaubey
Affiliation:
Nematology Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut250004, UP, India
V. Půža
Affiliation:
Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05České Budějovice, Czech Republic
J. Abolafia
Affiliation:
Laboratorio de Nematología, Departamento de Biología Animal, Biol©ogía Vegetal y Ecología, Universidad de Jaén, Avenida de Ben Saprut s/n, 23071Jaén, Spain
*
Author for correspondence: A.H. Bhat, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

A population of a nematode species belonging to the genus Oscheius was isolated in western Uttar Pradesh, India. Morphological and morphometrical studies on this species showed its high similarity with six species described previously from Pakistan (Oscheius citri, O. cobbi, O. cynodonti, O. esculentus, O. punctatus and O. sacchari). The molecular analysis of the ITS1-5.8S-ITS2 rDNA sequences of the Indian population and the six species described from Pakistan showed that all the sequences are almost identical. Thus, based on morphological and molecular characteristics, all of the six above-mentioned Pakistani species and Indian strain do not differ from each other, hence can be considered synonyms. The correct name for this taxon is the first described species O. citri. Additionally, the phylogenetic analysis of the 18S rDNA and the 28S rDNA sequences showed that Oscheius citri is sister to the clade formed by O. chongmingensis and O. rugaoensis from China. The high similarity of morphological and morphometric characteristics of O. citri and other species, O. maqbooli, O. nadarajani, O. niazii, O. shamimi and O. siddiqii, suggest their conspecificity; however, lack of molecular data for these species does not allow this hypothesis to be tested.

Keywords

18S rDNA28S rDNAITS rDNAmolecular analysismorphologyOscheius citriO. cobbiO. cynodontiO. esculentusO. punctatusO. saccharitaxonomy
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e24
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally.

References

Abolafia, J (2015) A low-cost technique to manufacture a container to process meiofauna for scanning electron microscopy. Microscopy Research and Technique 78, 771–776.CrossRefGoogle ScholarPubMed
Abolafia, J and Peña-Santiago, R (2017) On the identity of Chiloplacus magnus Rashid & Heyns, 1990 and C. insularis Orselli & Vinciguerra, 2002 (Rhabditida: Cephalobidae), two confusable species. Nematology 19, 1017–1034.CrossRefGoogle Scholar
Abolafia, J and Peña-Santiago, R (2019) Morphological and molecular characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) from decaying wood in spain, with new insights into the phylogeny of the genus and a revision of its taxonomy. Journal of Nematology 51, e2019–53.CrossRefGoogle Scholar
Ali, SS, Asif, M and Shaheen, A (2011) Oscheius nadarajani sp. n. (Nematoda: Rhabditida) from Lentil (Lens culinaris) Rhizosphere in Unnao District of Uttar Pradesh, India. Trends in Biosciences 4, 98–100.Google Scholar
Altschul, SF, Gish, W, Miller, W, Myers, EW and Lipman, DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215, 403–410.CrossRefGoogle ScholarPubMed
Andrássy, I (1976) Evolution as a basis for the systematization of nematodes. Budapest, London, San Francisco, Pitman Publishing, 288 pp.Google Scholar
Andrássy, I (1983) A taxonomic review of the suborder rhabditina (Nematoda: Secernentia). Paris, ORSTOM.Google Scholar
Bharti, L, Bhat, AH, Chaubey, AK and Abolafia, J (2020) Morphological and molecular characterization of Merlinius brevidens (Allen, 1955) Siddiqi, 1970 (Rhabditida, Tylenchomorpha) on mustard crop from Uttar Pradesh, India. Journal of Natural History 54, 1477–1498.CrossRefGoogle Scholar
Bhat, AH, Istkhar, , Chaubey, AK, Půža, V and San-Blas, E (2017) First report and comparative study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its symbiont bacteria from subcontinental India. Journal of Nematology 49, 92–102.CrossRefGoogle ScholarPubMed
Bhat, AH, Chaubey, AK, Shokoohi, E and Mashela, PW (2019a) Study of Steinernema hermaphroditum (Nematoda, Rhabditida) from the West Uttar Pradesh, India. Acta Parasitologica 64, 720–737.CrossRefGoogle Scholar
Bhat, AH, Chaubey, AK and Půža, V (2019b) The first report of Xenorhabdus indica from Steinernema pakistanense: Co-phylogenetic study suggests co-speciation between X. indica and its steinernematid nematodes. Journal of Helminthology 92, 81–90.CrossRefGoogle Scholar
Bhat, AH, Rana, A, Chaubey, AK, Machado, RAR and Abolafia, J (2020) Morphological, morphometrical, and molecular characterization of Metarhabditis amsactae (Ali, Pervez, Andrabi, Sharma and Verma, 2011) Sudhaus, 2011 (Rhabditida, Rhabditidae) from India and proposal of Metarhabditis longicaudata as a junior synonym of M. amsactae. Journal of Nematology 52, e2020-116.CrossRefGoogle Scholar
Bhat, AH, Chaubey, AK, Hartmann, J, Nermuť, J and Půža, V (2021a) Notes on the morphology, bionomics, distribution and efficacy of Steinernema siamkayai (Rhabditida: Steinernematidae) from Western Uttar Pradesh, India. Nematology 23, 1–20.CrossRefGoogle Scholar
Bhat, AH, Chaubey, AK, Shokoohi, E and Machado, RAR (2021b) Molecular and phenotypic characterization of Heterorhabditis indica (Nematoda: Rhabditida) nematodes isolated during a survey of agricultural soils in Western Uttar Pradesh, India. Acta Parasitologica 66, 236–252.CrossRefGoogle Scholar
Blaxter, ML, De Ley, P, Garey, JR, et al. (1998) A molecular evolutionary framework for the phylum Nematoda. Nature 392, 71–75.CrossRefGoogle ScholarPubMed
Campos-Herrera, R, Jaffuel, G, Chiriboga, X, Blanco-Peréz, R, Fesselet, M, Půža, V, Mascher, F and Turlings, TJC (2015) Traditional and molecular detection methods reveal intense inter guild competition and other multi trophic interactions associated with native entomopathogenic nematodes in Swiss tillage soils. Plant and Soil 389, 237–255.CrossRefGoogle Scholar
Courtney, WD, Polley, D and Miller, VI (1955) TAF an improved fixative in nematode technique. Plant Disease Reporter 39, 570–571.Google Scholar
Darsouei, R, Karimi, J and Shokoohi, E (2014) Oscheius rugaoensis and Pristionchus maupasi, two new records of entomophilic nematodes from Iran. Russian Journal of Nematology 22, 141–155.Google Scholar
De Ley, P and Blaxter, M (2002) Systematic position and phylogeny. pp. 1–30 in Lee, D (Ed) The biology of nematodes. London, Taylor and Francis.Google Scholar
De Ley, P, van de Velde, MC, Mounport, D, Baujard, P and Coomans, A (1995) Ultrastructure of the stoma in Cephalobidae, Panagrolaimidae and Rhabditidae, with a proposal for a revised stoma terminology in Rhabditida (Nematoda). Nematologica 41, 153–182.CrossRefGoogle Scholar
Hall, TA (1999) Bioedit: A user–friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.CrossRefGoogle ScholarPubMed
Kumar, P, Jamal, W, Somvanshi, VS, Chauban, K and Mumtaz, S (2019) Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India. Journal of Nematology 51, 1–11.CrossRefGoogle Scholar
Liu, J, Berry, RE and Moldenke, AF (1997) Phylogenetic relationships of entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) inferred from partial 18S rRNA gene sequences. Journal of Invertebrate Pathology 69, 246–252.CrossRefGoogle ScholarPubMed
Liu, QZ, Mráček, Z, Zhang, LJ, Půža, V and Dong, LM (2012) Re-description of Oscheius chongmingensis (Zhang et al., 2008) (Nematoda: Rhabditidae) and its entomopathogenicity. Nematology 14, 139–149.CrossRefGoogle Scholar
Mráček, Z, Půža, V and Nermuť, J (2014) Steinernema poinari sp. n. (Nematoda: Steinernematidae) a new entomopathogenic nematode from Czech Republic. Zootaxa 3760, 336–350.CrossRefGoogle Scholar
Nguyen, KB (2007) Methodology, morphology and identification. p. 816 in Nguyen, KB, Hunt, DJ (Eds) Nematology monographs and perspectives 5. Entomopathogenic nematodes: Systematics, phylogeny and bacterial symbionts. Brill, Leiden.CrossRefGoogle Scholar
Nylander, JAA (2004) Mrmodeltest 2.0. Program distributed by the author. Sweden, Uppsala University, Evolutionary Biology Centre.Google Scholar
Pervez, R, Eapen, SJ, Devasahayam, S and Jacob, TK (2013) A new species of entomopathogenic nematode Oscheius gingeri sp. n. (Nematoda: Rhabditidae) from ginger rhizosphere. Archives of Phytopathology and Plant Protection 46, 526–535.CrossRefGoogle Scholar
Rana, A, Bhat, AH, Chaubey, AK, Suman, B and Abolafia, J (2020) Morphological and molecular characterization of Acrobeloides saeedi Siddiqi, De Ley and Khan, 1992 (Rhabditida, Cephalobidae) from India and comments on its status. Journal of Nematology 52, e2020-27.CrossRefGoogle Scholar
Ronquist, F, Teslenko, M, Van Der Mark, P, et al. (2012) Mrbayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539–542.CrossRefGoogle ScholarPubMed
Seinhorst, JW (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67–69.CrossRefGoogle Scholar
Suman, B, Bhat, AH, Aasha, R, Chaubey, AK and Abolafia, J (2020) Morphological and molecular characterisation of Distolabrellus veechi (Rhabditida: Mesorhabditidae) from India. Nematology 22, 439–452.CrossRefGoogle Scholar
Tabassum, KA and Shahina, F (2002) Oscheius maqbooli n. sp. and observations on three known rhabditid species (Nemata: Rhabditida) from sugarcane fields of Balochistan, Pakistan. Pakistan Journal of Nematology 20, 1–21.Google Scholar
Tabassum, KA and Shahina, F (2008) Oscheius andrassyi sp.n. (Nematoda: Rhabditidae) with its key and embryonic and postembryonic development from Jhang, Pakistan. Pakistan Journal of Nematology 26, 125–140.Google Scholar
Tabassum, KA and Shahina, F (2010) Oscheius siddiqii and O. niazii, two new entomopathogenic nematode species from Pakistan, with observation on O. shamimi. International Journal of Nematology 201, 75–84.Google Scholar
Tabassum, KA, Shahina, F, Nasira, K and Erum, YI (2016) Description of six new species of Oscheius Andrassy, 1976 (Nematoda: Rhabditida) from Pakistan with a key and diagnostic compendium to species of the genus. Pakistan Journal of Nematology 34, 109–161.Google Scholar
Tahseen, Q and Nisa, SU (2006) Embryology and gonad development in Oscheius shamimi sp. n. (Nematoda: Rhabditida). Nematology 8, 211–221.CrossRefGoogle Scholar
Vrain, TC, Wakarchuk, DA, Levesque, AC and Hamilton, RI (1992) Intraspecific rDNA restriction fragment length polymorphisms in the Xiphinema americanum group. Fundamental and Applied Nematology 15, 563–574.Google Scholar
White, GF (1927) A method for obtaining infective nematode larvae from cultures. Science 66, 302–303.CrossRefGoogle ScholarPubMed
Ye, W, Torres-Barragan, A and Cardoza, YJ (2010) Oscheius carolinensis n. sp. (Nematoda: Rhabditidae), a potential entomopathogenic nematode from vermicompost. Nematology 12, 121–135.Google Scholar
Zhang, K, Baiocchi, T, Lu, D, Chang, DZ and Dillman, AR (2019) Differentiating between scavengers and entomopathogenic nematodes: Which is Oscheius chongmingensis? Journal of Invertebrate Pathology 167, 107245.CrossRefGoogle ScholarPubMed
Zhang, C, Liu, J, Xu, M, et al. (2008) Heterorhabditidoides chongmingensis gen. nov., sp. nov. (Rhabditida: Rhabditidae), a novel member of the entomopathogenic nematodes. Journal of Invertebrate Pathology 98, 153–168.CrossRefGoogle Scholar