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Radiolabelling of the excretory-secretory and somatic antigens of Anisakis simplex larvae

Published online by Cambridge University Press:  05 June 2009

Kazuo Sugane ,
Shu-Han Sun  and
Tadashi Matsuura
Kazuo Sugane
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Matsumoto City, Nagano Prefecture 390, Japan
Shu-Han Sun
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Matsumoto City, Nagano Prefecture 390, Japan
Tadashi Matsuura
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Matsumoto City, Nagano Prefecture 390, Japan
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Abstract

Anisakis simplex larvae were cultured in vitro in medium containing 35-methionine for ten days. The medium and the larval tissues were analysed for biosynthetically labelled polypeptide by sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography. Immunoprecipitates with positive and negative human antisera were similarly analysed, using Staphylococcus aureus to absorb immunocomplexes. ES products of Anisakis larvae contained many polypeptides with molecular weights of less than 200 K. 180 KDa and 40 KDa polypeptides in ES products reacted with IgG in Anisakis-infected human sera. Somatic extracts also contained many polypeptides with molecular weights of less than 200 K. One of these polypeptides with a molecular weight of 130 K reacted with IgG in Anisakis-infected human sera. These polypeptides did not react with other nematode-infected human sera.

Keywords

Anisakis simplexlarvaeES antigensomatic antigenradiolabellingNematoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 4 , December 1992 , pp. 305 - 309
Copyright
Copyright © Cambridge University Press 1992

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