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Purification and characterization of a glycoprotein from the surface of Ascaridia galli*

Published online by Cambridge University Press:  05 June 2009

Khalid Masood ,
K. P. Sircar  and
V. M. L. Srivastava
Khalid Masood
Affiliation:
Central Drug Research Institute, Lucknow 226001, India
K. P. Sircar
Affiliation:
Division of Biochemistry and HPLC UnitLucknow 226001, India
V. M. L. Srivastava
Affiliation:
Central Drug Research Institute, Lucknow 226001, India
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Abstract

Employing papain as the enzyme and agarose bound Ricinus communis agglutinin as the affinity gel, a glycoprotein has been isolated and purified from the surface of Ascaridia galli. The glycoprotein shows an apparent molecular weight of 68 kilo daltons and contains fucose, galactose, rhamnose and glucosamine as sugar moieties. Only 2% of its entire molecule has been found to possess α-helical configuration.

Keywords

Ascaridia galliglycoproteinsurface
Type
Research Papers
Information
Journal of Helminthology , Volume 61 , Issue 3 , September 1987 , pp. 219 - 224
Copyright
Copyright © Cambridge University Press 1987

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