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Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome

Published online by Cambridge University Press:  28 August 2018

J.R. Ambrosio ,
M.I. Palacios-Arreola ,
D.G. Ríos-Valencia ,
O. Reynoso-Ducoing ,
K.E. Nava-Castro ,
P. Ostoa-Saloma  and
J. Morales-Montor Open the ORCID record for J. Morales-Montor [Opens in a new window]
J.R. Ambrosio
Affiliation:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico (UNAM), Edificio A, 2do piso, Ciudad Universitaria, Ciudad de México, 04510, Mexico
M.I. Palacios-Arreola
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de México, 04510, Mexico Laboratorio de Genotoxicología y Mutagénesis Ambiental, Departamento de Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México. Ciudad de México, 04510, Mexico
D.G. Ríos-Valencia
Affiliation:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico (UNAM), Edificio A, 2do piso, Ciudad Universitaria, Ciudad de México, 04510, Mexico
O. Reynoso-Ducoing
Affiliation:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico (UNAM), Edificio A, 2do piso, Ciudad Universitaria, Ciudad de México, 04510, Mexico
K.E. Nava-Castro
Affiliation:
Laboratorio de Genotoxicología y Mutagénesis Ambiental, Departamento de Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México. Ciudad de México, 04510, Mexico
P. Ostoa-Saloma
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de México, 04510, Mexico
J. Morales-Montor*
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, Ciudad de México, 04510, Mexico
*
Author for correspondence: J. Morales-Montor E-mail: [email protected][email protected]
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Abstract

Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10–250 kDa and isoelectrical point range of 3–10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 5 , September 2019 , pp. 539 - 547
Copyright
Copyright © Cambridge University Press 2018 

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